Is there a specific surface marker that identifies human endometrial epithelial progenitor cells with adult stem cell activity using in vitro assays? N-cadherin isolates clonogenic, self-renewing high proliferative potential differentiate into cytokeratin+ gland-like structures and their location some of gland profiles predominantly basalis endometrium adjacent to the myometrium. Human contains small population large structures, but identity is unknown. Stage-specific embryonic antigen-1...

Abstract Endometriosis is a common inflammatory disorder in women of reproductive age due to an abnormal endometrial immune environment and associated with infertility. This study aimed systematically understand the leukocyte types, environment, impaired receptivity at single-cell resolution. We profiled RNA transcriptomes 138 057 cells from endometriosis patients (n = 6) control 7), respectively, using 10x Genomics platform. found that one cluster epithelial expressed PAEP CXCL14 was mostly...

The imbalance in cell proliferation and apoptosis is considered an important role the pathogenesis of endometriosis, but exact mechanisms remains unclear. A newly established signaling pathway–Hippo/Yes-associated protein (YAP) pathway plays a critical processes. However, studies focusing on Hippo/YAP endometriosis are lacking.The objective was to explore function endometriosis.The expression YAP first investigated endometrium women with or without endometriosis. identified by transfection...

Do microRNAs (miRNAs) contribute to aberrant progesterone receptor (PGR) expression in the eutopic endometrium of women with endometriosis? miR-196a upregulates MEK/ERK signalling, mediating a downregulation PGR minimal or mild endometriosis. Implantation failure is strongly suggested as an underlying cause for observed infertility Progesterone resistance, which mainly caused by aberrantly expressed endometrium, considered key factor decreased endometrial receptivity; thus far, epigenetics,...

Progesterone resistance in the eutopic endometrium (EuE) is suggested to be a critical factor for decreased endometrial receptivity and implantation failure reproductive-aged women with endometriosis. Altered expression of miRNAs has been reported play an important role pathophysiology endometriosis-associated infertility. However, underlying mechanisms aberrant progesterone receptor (PR) deficient decidualization regulated by endometriosis have not thoroughly elucidated. The goal this study...

Activation of inflammation-mediated endometrial injury is suggested to play a decisive role in pathogenesis intrauterine adhesion (IUA). The stem cell theory diseases has been given hotspot, that human cells have isolated from the endometrium. Three transcription factors key roles maintaining pluripotency and self-renewal are sex-determining region Y-box2 (SOX2), Nanog homebox (NANOG), octamer-binding protein (OCT4), which may be responsible for damage or repair process uterine We aim...

Sepsis is a potentially fatal condition with significant risk of death. Acute lung injury (ALI) life-threatening complication sepsis, and the inflammatory response plays critical role in sepsis-induced ALI. The protective effects micheliolide (MCL) against renal fibrosis leukemia have been demonstrated, but precise underlying mechanisms remain unclear. In vitro, lipopolysaccharides (LPS) interferon-gamma (IFN-γ) were used to stimulate RAW264.7 cells bone marrow-derived macrophages (BMDMs)...

The precise etiology of endometriosis is not fully understood; the involvement stem cells theory a new hypothesis. Related studies mainly focus on stemness-related genes, and pluripotency markers may play role in endometriosis. We aimed to analyze transcription factors sex-determining region Y-box 2 (SOX2), Nanog homeobox (NANOG), octamer-binding protein 4 (OCT4) endometrium reproductive-age women with without ovarian recruited 26 laparoscopy-diagnosed (endometriosis group) 16 disease-free...

The beneficial effects of metformin, especially its capacity to ameliorate insulin resistance (IR) in polycystic ovary syndrome (PCOS), explains why it is widely prescribed. However, effect on the offspring patients with PCOS remains uncertain. This study investigated impact metformin treatment first- and second-generation female born letrozole-induced PCOS-IR rats. Forty-five Wistar rats were implanted continuous-release letrozole pellets or placebo treated vehicle control. Rats exposed...

We first reported that the Hippo-YAP signaling pathway plays a critical role in pathogenesis of endometriosis (EMS). Autophagy is also related to invasion ability endometrial cells and involved EMS through multi-levels. However, precise regulatory mechanism YAP on autophagy eutopic stromal (ESCs) still unclear. Primary ESCs patients ( n = 12) control without 9) were isolated cultured investigate expressions mTOR, autophagy, effect YAP-autophagy signal decidualization ESCs....

Protein prenylation occurs in the protozoan that causes African sleeping sickness (Trypanosoma brucei), and protein farnesyltransferase appears to be a good target for developing drugs. We have cloned α- β-subunits of T. brucei (TB-PFT) using nucleic acid probes designed from partial amino sequences obtained enzyme purified insect stage parasites. TB-PFT is expressed both bloodstream Enzymatically active was produced by heterologous expression Escherichia coli. Compared with mammalian...

While combined oral contraceptives (COCs) are commonly used to treat polycystic ovary syndrome (PCOS), comparative data regarding metabolic effects of different progestogens on this patient population missing. This study aimed compare the drospirenone (DRP)-containing COCs with cyproterone acetate (CPA)-containing COCs, metformin and lifestyle modifications in women PCOS disorders.Ninety-nine a disorder between January 2011 2013 were enrolled into prospective randomized clinical trial....

Abstract Background Hydrosalpinx are associated with infertility, due to reduced rates of implantation and increased abortion rates. The aims this study were investigate the expression cystic fibrosis transmembrane conductance regulator (CFTR), nuclear factor kappa B (NF KappaB) mucin-1 (MUC-1), analyze correlation between CFTR NF KappaB or MUC1, in endometrium infertile women without hydrosalpinx. Methods Thirty-one laparoscopy-confirmed unilateral bilateral hydrosalpinx 20 pelvic...

Based on microRNA (miR) microarray analysis, we previously found that miR22-5p expression is decreased in the mid-luteal endometrium of women with minimal/mild endometriosis. Bioinformatics analysis predicted targets ten-eleven translocation (TET2) 3'-untranslated region. This study aimed to determine regulation and roles pathogenesis endometriosis-associated infertility. MiR22-5p TET2 from or without endometriosis was analyzed. After transfection mimics inhibitor, analyzed by quantitative...

We investigated the possible association between sterol regulatory element-binding protein-1c gene (SREBP-1c) rs2297508 polymorphism and changes in lipid profiles a high-carbohydrate low-fat (high-CHO/LF) diet Chinese population well characterized by lower incidence of coronary heart disease featuring higher carbohydrate fat. Fifty-six healthy youth (aged 22.89 ± 1.80 years) were given wash-out diets 31% fat 54% for 7 days, followed high-CHO/LF 15% 70% 6 without total energy restriction....

Abstract Introduction Sufficient uterine blood supply is essential for the fetus to develop normally in uterus. Several mechanisms are involved process of vessel development deciduas and villus. We focus on whether first-trimester decidua side population (SP) cells contain capable differentiating into endothelial cells. Methods Eight samples were collected from healthy women, 22- 30-years old, undergoing elective terminations early pregnancy (six eight gestational weeks). The cell...

Abstract Rationale: Klippel–Trenaunay Syndrome (KTS) is a congenital vascular disease characterized by cutaneous hemangiomas, venous varicosities, and limb hypertrophy. Although extremely rare in pregnant women, the present alterations may be aggravated, consequent to postural hormonal changes inherent pregnancy. Pregnancy not advised KTS women due increased obstetrical risk. Patient concerns: A 31-year-old pregnancy woman presented with prominent vascularity pelvis, right lower limb,...

The status of the maternal endometrium is vital in regulating humoral homeostasis and for ensuring embryo implantation. Cystic fibrosis transmembrane conductance regulators (CFTR) epithelial sodium channel alpha subunits (ENaC-α) play an important role female reproduction by maintaining cell homeostasis. However, it not clear whether expression levels CFTR ENaC-α decidual component during early pregnancy are related with miscarriage. CBA×DBA/2 mouse mating has been widely accepted as a...

The human endometrium is a dynamic tissue that undergoes approximately 400 cyclical episodes of proliferation, differentiation, shedding, and regeneration in woman's reproductive lifespan. regenerative capacity likely mediated by adult stem cells. At the cellular level, endometrial mesenchymal stem/stromal cells, located both functionalis basalis layers, support stromal vascular compartment epithelial progenitor postulated to reside epithelium, regenerate glands. Bone marrow including...

Both genetic background and diet have profound effects on plasma lipid profiles. We hypothesized that a high-carbohydrate (high-CHO) may affect the ratios of serum lipids apolipoproteins (apo) differently in subjects with different genotypes SstI polymorphism apoCIII gene (APOC3). Fifty-six healthy university students (27 males 29 females, 22.89 ± 1.80 years) were given washout 54% carbohydrate for 7 days, followed by high-CHO 70% 6 days without total energy restriction. Serum triglyceride...