A5083696279- Diagnosis and Treatment of Venous Diseases
- Venous Thromboembolism Diagnosis and Management
- Trauma Management and Diagnosis
- Central Venous Catheters and Hemodialysis
- Infectious Aortic and Vascular Conditions
- Aortic aneurysm repair treatments
- Aortic Disease and Treatment Approaches
- Autophagy in Disease and Therapy
- Cardiac Valve Diseases and Treatments
- Tracheal and airway disorders
- Infective Endocarditis Diagnosis and Management
- Cardiac Arrest and Resuscitation
- Nicotinic Acetylcholine Receptors Study
- Vascular Procedures and Complications
- Peripheral Artery Disease Management
- Growth Hormone and Insulin-like Growth Factors
- Cardiac Structural Anomalies and Repair
- Diabetes Treatment and Management
- Trauma and Emergency Care Studies
- Metabolism, Diabetes, and Cancer
- Antimicrobial Resistance in Staphylococcus
- Vascular anomalies and interventions
- Streptococcal Infections and Treatments
- Angiogenesis and VEGF in Cancer
- Ion channel regulation and function
Houston Methodist
2025
Chiayi Chang Gung Memorial Hospital
2015-2024
Chang Gung University
2014-2024
Second Xiangya Hospital of Central South University
2011-2024
Central South University
2011-2024
Ministry of Health and Welfare
2024
Zhejiang Provincial People's Hospital
2024
Army Medical University
2011-2023
Xinqiao Hospital
2023
Taipei City Hospital
2023
Abstract Background Although a variety of methods and expensive kits are available, molecular cloning can be time-consuming frustrating process. Results Here we report highly simplified, reliable, efficient PCR-based technique to insert any DNA fragment into plasmid vector or gene (cDNA) in at desired position. With this method, the PCR amplified separately, with only 18 cycles, using high fidelity polymerase. The has ends ~16-base overlapping vector. After Dpn I digestion mixture eliminate...
Nicotinic acetylcholine receptors (nAChRs) containing α7 subunits are thought to assemble as homomers. α7-nAChR function has been implicated in learning and memory, alterations of have found patients with Alzheimer's disease (AD). Here we report findings consistent a novel, naturally occurring nAChR subtype rodent, basal forebrain cholinergic neurons. In these cells, coexpressed, colocalize, coassemble β2 subunit(s). Compared homomeric α7-nAChRs from ventral tegmental area neurons,...
Lysosomal membrane permeabilization (LMP) has recently been recognized as an important cell death pathway in various types. However, studies regarding the correlation between LMP and cardiomyocyte are scarce. membrane-associated protein 2 (Lamp2) is component of lysosomal membranes involved both autophagy LMP. In present study, we found that content Lamp2 gradually decreased response to oxygen, glucose serum deprivation (OGD) treatment vitro. To further elucidate its role ischemic...
Peroxisome proliferator-activated receptor β/δ (PPARβ/δ) is an essential transcription factor in myocardial metabolism. This study aims to investigate the effects of PPARβ/δ activation adult heart on mitochondrial biology and oxidative metabolism under normal pressure-overload conditions. We have investigated cardiac constitutively active mice using a tamoxifen-inducible transgenic approach with Cre-LoxP recombination. The expression mRNA protein cardiomyocytes was substantially increased...
β-amyloid (Aβ) accumulation is described as a hallmark of Alzheimer's disease (AD). Aβ perturbs number synaptic components including nicotinic acetylcholine receptors containing α7 subunits (α7-nAChRs), which are abundantly expressed in the hippocampus and found on GABAergic interneurons. We have previously demonstrated existence novel, heteromeric α7β2-nAChR basal forebrain cholinergic neurons that exhibits high sensitivity to acute exposure. To extend our previous work, we evaluated...
Background: Mycotic aortic aneurysm (MAA) is an infrequent but devastating form of vascular disease. Methods: We conducted a retrospective cohort study at major medical center to identify independent risk factors for MAA and provide opinions about treating it. The population consisted 43 patients who had 44 MAAs over period 15 y. Results: All the positive blood cultures, radiologic findings typical MAA, clinical signs infection (leukocytosis, fever, elevated C-reactive protein). mean age was...
There is no consensus regarding the terminology, definition, classification, diagnostic criteria, and algorithm, or reporting standards for disease of infective native aortic aneurysm (INAA), previously known as mycotic aneurysm. The aim this study was to establish by performing a study.The Delphi methodology used. Thirty-seven international experts were invited via mail participate. Four two week rounds performed, using an online questionnaire, initially with 22 statements nine items....
Growth hormone (GH) promotes signaling by causing activation of the non-receptor tyrosine kinase, JAK2, which associates with GH receptor. causes phosphorylation epidermal growth factor receptor (EGFR; ErbB-1) and its family member, ErbB-2. For EGFR, JAK2-mediated GH-induced may allow EGFR to serve as a scaffold for signaling. ErbB-2, induces serine/threonine that dampens basal EGF-induced ErbB-2 kinase activation. We now further explore in 3T3-F442A, preadipocytic fibroblast cell line...
Extracorporeal life support (ECLS) has been applied successfully to patients with cardiopulmonary failure in extreme situations. Refractory ventricular tachycardia high mortality and morbidity rates if not terminated time. This study describes our preliminary experiences using ECLS treat refractory tachycardia.Retrospective chart review.Hospital.Eleven suffering from antiarrhythmia agents cardioversion attempts.From January 2002 December 2004, 11 attempts were treated ECLS. Mean patient age...
The exact relationships and detailed mechanisms between autophagy necroptosis remain obscure. Here, we demonstrated the link accumulated autophagosome by intervening with autophagic flux. We first confirmed that LC3 interacting region (LIR) domain is present in protein sequences of RIPK1 RIPK3. Mutual effects among LC3, RIPK1, RIPK3 have been identified myocardium cardiomyocytes. Direct LC3-RIPK1 LC3-RIPK3 interactions were pull-down assays, their deleted after LIR mutation. Moreover,...
Introduction Staphylococcus aureus , is a pathogen commonly encountered in both community and hospital settings. Patients receiving hemodialysis treatment face an elevated risk of vascular access infections (VAIs) particularly infection. This heightened attributed to the characteristics enabling it adhere suitable surfaces form biofilms, thereby rendering resistant external interventions complicating efforts. Methods Therefore this study utilized PCR microtiter dish biofilm formation assay...
Abstract Bladder cancer (BLCA) is the 6th most commonly diagnosed in US. Despite recent advances, BLCA has a high recurrence rate. Increased deposition of extracellular matrix Collagen I (Col I) hallmark progression and correlates with poor patient survival BLCA. Recent studies have showcased dynamic nature Col I, which signals key pro-tumoral processes like immune evasion metastasis. Interestingly, limits nutrient access oxygen to adjacent tumors tumor microenvironment. Cancer cells adapt...
Abstract: The recent introduction of the chloronicotinyl insecticide imidacloprid, targeting insect nicotinic acetylcholine receptors (nAChRs), emphasises importance a detailed molecular characterisation these receptors. We are investigating diversity nAChR subunit genes in an important agricultural pest, peach‐potato aphid Myzus persicae . Two M. α‐subunit cDNAs, Mpα1 and Mpα2, have been cloned previously. Here we report isolation three novel (Mpα3‐5) with overall amino acid sequence...
The growth hormone receptor (GHR) is a cell surface that mediates the somatogenic and metabolic effects of (GH). GHR signaling transduced via receptor-associated cytoplasmic tyrosine kinase called Janus protein 2 (JAK2). major intracellular systems activated by JAK2 in response to GH include signal transducer activator transcription (STAT) 5 extracellular signal-regulated (ERK)-1 -2 pathways. In this report, we investigate role cholesterol-rich plasma membrane microdomains (caveolae lipid...
Aspiration of the lower airways due to foreign body is rare in adults. This study aimed determine outcome patients who received flexible bronchoscopy with different modalities for removal airways.Between January 2003 and 2014, 94 diagnosed underwent modalities, which included forceps, loop, basket, knife, electromagnet, cryotherapy. The clinical presentation, location characteristics, applications were analyzed.Forty (43%) had acute aspiration, developed within one week entry 54 (57%)...