A5034460689- Bladder and Urothelial Cancer Treatments
- Cancer Immunotherapy and Biomarkers
- Renal cell carcinoma treatment
- Epigenetics and DNA Methylation
- Urinary and Genital Oncology Studies
- Cancer Cells and Metastasis
- Cytokine Signaling Pathways and Interactions
- PI3K/AKT/mTOR signaling in cancer
- Ferroptosis and cancer prognosis
- Cancer Research and Treatments
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Tissue Engineering and Regenerative Medicine
- Mathematical Biology Tumor Growth
- Melanoma and MAPK Pathways
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Protein Degradation and Inhibitors
- Molecular Biology Techniques and Applications
- Lymphoma Diagnosis and Treatment
- Multiple Myeloma Research and Treatments
- Computational Drug Discovery Methods
- IL-33, ST2, and ILC Pathways
Houston Methodist
2023-2025
Cedars-Sinai Medical Center
2019-2023
Methodist Hospital
2023
University of York
2023
University of Maryland, College Park
2023
Samueli Institute
2022
Baylor College of Medicine
2012-2021
Children's Cancer Center
2019
Baylor University
2019
Kelsey-Seybold Clinic
2016
Muscle-invasive bladder cancer (MIBC) is a molecularly diverse disease with heterogeneous clinical outcomes. Several molecular classifications have been proposed, but the diversity of their subtype sets impedes application. To achieve an international consensus on MIBC subtypes that reconciles published classification schemes. We used 1750 transcriptomic profiles from 16 datasets and two additional cohorts. performed network-based analysis six independent systems to identify set classes....
Major clinical issues in bladder cancer include the identification of prediction markers and novel therapeutic targets for invasive cancer. In current study, we describe isolation characterization a tumor-initiating cell (T-IC) subpopulation primary human cancer, based on expression similar to that normal basal cells (Lineage-CD44(+)CK5(+)CK20(-)). The T-IC was defined functionally by its enriched ability induce xenograft tumors vivo recapitulated heterogeneity original tumor. Further,...
Current clinical judgment in bladder cancer (BC) relies primarily on pathological stage and grade. We investigated whether a molecular classification of tumor cell differentiation, based developmental biology approach, can provide additional prognostic information. Exploiting large preexisting gene-expression databases, we developed biologically supervised computational model to predict markers that correspond with BC differentiation. To mechanistic insight, assessed relative tumorigenicity...
Identification of clinically relevant tumor subtypes and omics signatures is an important task in cancer translational research for precision medicine. Large-scale genomic profiling studies such as The Cancer Genome Atlas (TCGA) Research Network have generated vast amounts genomic, transcriptomic, epigenomic, proteomic data. While these provided great resources researchers to discover driver molecular alterations, there are few computationally efficient methods tools integrative clustering...
The advent of Omics technologies has been key to the molecular subclassification urothelial bladder cancer. Several groups have used different strategies this aim, with partially overlapping findings. meeting at Spanish National Cancer Research Center-CNIO was held discuss such classifications and reach consensus where appropriate. After updated presentations on work performed by teams attending meeting, a reached regarding existence group Basal-Squamous-like tumors - designated BASQ...
Abstract Induction of tumor cell death is the therapeutic goal for most anticancer drugs. Yet, a mode drug-induced death, known as immunogenic (ICD), can propagate antitumoral immunity to augment efficacy. Currently, molecular hallmark ICD features release damage-associated patterns (DAMPs) by dying cancer cells. Here, we show that gemcitabine, standard chemotherapy various solid tumors, triggers immunostimualtory DAMP (e.g., calreticulin, HSP70, and HMGB1); however, unable induce ICD....
Abstract Neoadjuvant chemotherapy (NAC) prior to surgery and immune checkpoint therapy (ICT) have revolutionized bladder cancer management. However, stratification of patients that would benefit most from these modalities remains a major clinical challenge. Here, we combine single nuclei RNA sequencing with spatial transcriptomics single-cell resolution proteomic analysis human identify an epithelial subpopulation therapeutic response prediction ability. These cells express Cadherin 12 (...
T cell-based immunotherapies have exhibited promising outcomes in tumor control; however, their efficacy is limited immune-excluded tumors. Cancer-associated fibroblasts (CAFs) play a pivotal role shaping the microenvironment and modulating immune infiltration. Despite identification of distinct CAF subtypes using single-cell RNA-sequencing (scRNA-seq), functional impact on hindering T-cell infiltration remains unclear, particularly soft-tissue sarcomas (STS) characterized by low response...
Constitutive activation of signal transducer and activator transcription 3 (Stat3) has been found in a wide spectrum human malignancies. Here, we have assessed the effect Stat3 deficiency on skin tumor development using 2-stage chemical carcinogenesis model. The epidermis Stat3-deficient mice showed significantly reduced proliferative response following treatment with promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) because defect G1-to-S-phase cell cycle progression. Treatment initiator...
Constitutive activation of signal transducer and activator transcription 3 (Stat3) has been found in a wide spectrum human malignancies. Here, we have assessed the effect Stat3 deficiency on skin tumor development using 2-stage chemical carcinogenesis model. The epidermis Stat3-deficient mice showed significantly reduced proliferative response following treatment with promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) because defect G1-to-S-phase cell cycle progression. Treatment initiator...
Significance Recruitment, proliferation, and differentiation of myofibroblasts are common in many disease states. Mechanisms that regulate proliferation poorly understood, although TGF-β is a key inducer differentiation. Here, we report, for the first time to our knowledge, runt-related transcription factor 1 (RUNX1) regulates mesenchymal stem cell (MSC) biology progenitor commitment myofibroblasts. In this work, describe identification, tissue-resident MSCs from adult normal human prostate...
Two subtypes of human bladder cancer, noninvasive papillary and muscle-invasive develop through independent pathologic molecular pathways. Human invasive cancer frequently develops without prior clinical evidence a tumor stage. However, an animal model that recapitulates this unique progression has not yet been developed. In study, we created novel transgenic mouse by targeting active dimerized form Stat3 to the basal cells epithelium. When exposed carcinogen nitrosamine, Stat3-transgenic...
Abstract Decades of research into the molecular mechanisms cancer and development novel therapeutics have yielded a number remarkable successes. However, our ability to broadly assign effective, rationally targeted therapies in personalized manner remains elusive for many patients, drug resistance persists as major problem. This is part due well-documented heterogeneity cancer, including diversity tumor cell lineages states, spectrum somatic mutations, complexity microenvironments,...
Colorectal cancer exhibits dynamic cellular and genetic heterogeneity during progression from precursor lesions toward malignancy. Analysis of spatial multi-omic data 31 human colorectal specimens enabled phylogeographic mapping tumor evolution that revealed individualized trajectories accompanying microenvironmental clonal alterations. Phylogeographic ordered events, classified tumors by their evolutionary dynamics, placed regions along global pseudotemporal encompassing the chromosomal...
Summary: Research on precancers, as defined at-risk tissues and early lesions, is of high significance given the effectiveness intervention. We discuss need for risk stratification to prevent overtreatment, an emphasis role genetic epigenetic aging when considering risk, importance integrating macroenvironmental factors with molecules cells in lesions normal developing effective intervention health policy strategies.
Abstract In the present study, we have investigated possible role of signal transducers and activators transcription (STATs), particularly Stat3, in mouse skin tumor promotion multistage carcinogenesis. Stat1, Stat5 were activated epidermis after treatment with different classes promoters, including 12-O-tetradecanoylphorbol-13-acetate (TPA), okadaic acid, chrysarobin. addition, constitutively tumors generated by two-stage carcinogenesis regimen using 7,12-dimethylbenz(a)anthracene as...