A5042574986- Ion Channels and Receptors
- Ion channel regulation and function
- Prion Diseases and Protein Misfolding
- Neurobiology and Insect Physiology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Postharvest Quality and Shelf Life Management
- Natural Compounds in Disease Treatment
- Histone Deacetylase Inhibitors Research
- Pain Mechanisms and Treatments
- Cardiac electrophysiology and arrhythmias
- Neurological diseases and metabolism
- Biotin and Related Studies
- Nerve injury and regeneration
- Phytochemicals and Antioxidant Activities
- Signaling Pathways in Disease
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Enzyme Structure and Function
- Trace Elements in Health
- Virus-based gene therapy research
- Biochemical Analysis and Sensing Techniques
- Hereditary Neurological Disorders
- Gastrointestinal motility and disorders
- RNA modifications and cancer
- Immune cells in cancer
Southern Medical University
2016-2023
Nanfang Hospital
2013-2023
Central People's Hospital of Zhanjiang
2023
Novartis Institutes for BioMedical Research
2019-2022
Novartis (Switzerland)
2011-2022
Zhujiang Hospital
2017
Novartis (United Kingdom)
2009-2012
Horsham Hospital
2009-2012
University of Leeds
2000-2010
AstraZeneca (United Kingdom)
2010
1 2-aminoethoxydiphenyl borate (2-APB) has been widely used to examine the roles of inositol 1,4,5-trisphosphate receptors (IP3Rs) and store-operated Ca2+ entry is an emerging modulator cationic channels encoded by transient receptor potential (TRP) genes. 2 Using Ca2+-indicator dye patch-clamp recording we first examined blocking effect 2-APB on human TRPC5 expressed in HEK-293 cells. 3 The concentration-response curve IC50 20 microM slope close 1.0, suggesting one molecule binds per...
Transient receptor potential melastatin (TRPM)3 is a calcium-permeable ion channel activated by the neurosteroid pregnenolone sulfate and positively coupled to insulin secretion in beta cells. Although vascular TRPM3 mRNA has been reported, there no knowledge of protein or its regulation function cardiovascular system.To determine relevance biology.TRPM3 expression was detected at levels contractile proliferating smooth muscle Calcium entry evoked sphingosine suppressed blocking antibody...
Stroke is the second leading cause of death worldwide, and oxidative stress plays a crucial role. Celastrol exhibits strong antioxidant properties in several diseases; however, whether it can affect oxidation cerebral ischemic-reperfusion injury (CIRI) remains unclear. This study aimed to determine celastrol could reduce damage during CIRI elucidate underlying mechanisms. Here, we found that attenuated by upregulating nuclear factor E2-related 2 (Nrf2). Using alkynyl-tagged liquid...
In a screen of potential lipid regulators transient receptor (TRP) channels, we identified sphingosine-1-phosphate (S1P) as an activator TRPC5. We explored the relevance to vascular biology because S1P is key cardiovascular signaling molecule. TRPC5 expressed in smooth muscle cells human vein along with TRPC1, which forms complex Importantly, also activates TRPC5-TRPC1 heteromultimeric channel. Because TRPC channels are linked neuronal growth cone extension, considered related concept for...
Here we explore the activation mechanisms of human TRPC5, a putative cationic channel that was cloned from region X chromosome associated with mental retardation. No basal activity evident but induced by carbachol stimulation muscarinic receptors independently Ca2+ release. This is 'receptor activation', as described for mouse TRPC5. In addition, and in absence receptor stimulation, extracellular gadolinium (0.1 mm) activated an effect mimicked 5-20 mm intracellular buffered. We refer to...
TRPC calcium channels are emerging as a ubiquitous feature of vertebrate cells, but understanding them is hampered by limited knowledge the mechanisms activation and identity endogenous regulators. We have revealed that one channels, TRPC5, strongly activated common lysophospholipids including lysophosphatidylcholine (LPC) but, contrast, not arachidonic acid. Although TRPC5 was stimulated agonists at G-protein-coupled receptors, LPC occurred downstream independently G-protein signaling. The...
Mesenchymal stem cell (MSCs)-derived small Extracellular Vesicles (sEVs) are considered as a new cell-free therapy for pain caused by nerve injury, but whether human placental mesenchymal cell-derived sEVs relieve in sciatic injury and its possible mechanism still unclear. In this study, we investigated the roles of hPMSCs-derived related mechanisms neuropathic pain.The spared (SNI) mouse model was employed. Intrathecal injection or miR-26a-5p agomir performed on seventh day modeling, to...
Abstract Background Inflammation often leads to the occurrence of chronic pain, and many miRNAs have been shown play a key role in development inflammatory pain. However, whether miR‐26a‐5p relieves pain induced by inflammation its possible mechanism are still unclear. Methods The complete Freund's adjuvant (CFA)‐induced mouse model was employed. Intrathecal or subcutaneous injection agomir performed after modeling study antinociceptive effect comparison different administration methods....
The calcium- and sodium-permeable transient receptor potential channel TRPC5 has an inhibitory role in neuronal outgrowth but the mechanisms governing its activity are poorly understood. Here we propose a mechanism involving calcium sensor-1 (NCS-1) protein. Inhibitory mutants of NCS-1 enhance neurite similarly. Mutant does not inhibit surface-expression generally suppresses activity, irrespective whether it is evoked by carbachol, store depletion, lanthanides or elevated intracellular...
The distribution of methicillin-resistant Staphylococcus aureus (MRSA) clones is dynamic and geographically unique. To understand the changing epidemiology MRSA infections in China, we performed a prospective, multicity surveillance study with molecular typing phenotypic analysis to determine association major prevalent their antimicrobial resistance profiles. A total 517 S. isolates collected between January 2009 March 2012 from six cities China were subjected antibiogram typing, including...
Cerebral ischemia/reperfusion (I/R) injury is closely related to dysfunctional glucose metabolism. Celastrol a bioactive compound that has been found exhibit neuroprotective effects in cerebral ischemia, while whether it can protect against I/R by regulating glycolysis remains unclear. The goal of this study investigate the role celastrol on and its underlying mechanisms transient middle artery occlusion (tMCAO) mice. Methods. To observe protective effect select optimal dosage for further...
The underlying pathophysiological mechanisms of cerebral ischemia reperfusion injury (CIRI) is intricate, and current studies suggest that neuron, astrocyte, microglia, endothelial cell, pericyte all have different phenotypic changes specific cell types after ischemic stroke. And microglia account for the largest proportion CIRI. Previous transcriptomic stroke typically focused on 24 hours CIRI, obscuring dynamics cellular subclusters throughout disease process. Therefore, traditional...
The association of the prion protein (PrP) with sphingolipid- and cholesterol-rich lipid rafts is instrumental in pathogenesis neurodegenerative diseases. Although glycosylphosphatidylinositol (GPI) anchor an exoplasmic determinant raft association, PrP remained raft-associated human neuronal cells even when GPI was deleted or substituted for a transmembrane indicating that ectodomain contains localization signal. transmembrane-anchored occurred independently Cu(II) binding as it failed to...
AimThe aim of the study was to determine potential for KV1 potassium channel blockers as inhibitors human neoinitimal hyperplasia.
The role of the N-terminal half prion protein (PrPC) in normal cellular function and pathology remains enigmatic. To investigate biological N-terminus PrP, we examined properties a construct murine PrP-DA, which is tethered to membrane by an uncleaved signal peptide retains glycosyl-phosphatidylinositol anchor. Human neuroblastoma SH-SY5Y cells expressing PrP-DA were more susceptible hydrogen peroxide copper induced toxicity than wtPrP cells. had increased level intracellular free radicals...
Prokineticin 2 (PROK2) is an inflammatory cytokine-like molecule expressed predominantly by macrophages and neutrophils infiltrating sites of tissue damage. Given the established role prokineticin signaling on gastrointestinal function, we have explored Prok2 gene expression in conditions tract assessed possible consequences gut physiology.Prokineticin was examined normal colitic tissues using qPCR immunohistochemistry. Functional responses to PROK2 were studied calcium imaging a novel...
Ischemic stroke is one of the leading causes death and disability for adults, which lacks effective treatments. Dietary intake n-3 polyunsaturated fatty acids (n-3 PUFAs) exerts beneficial effects on ischemic by attenuating neuron inflammation induced microglial activation. However, impact mechanism PUFAs astrocyte function during have not yet been well investigated. Our current study found that dietary decreased infarction volume improved neurofunction in mice model transient middle...
Isoform-specific ion channel blockers are useful for target validation in drug discovery and can provide the basis new therapeutic agents aid determination of physiological functions channels. The aim this study was to generate a specific blocker human TRPM3 channels as tool help investigations member TRP cationic family.A polyclonal antibody (TM3E3) made conserved peptide third extracellular (E3) loop tested binding functional effect. Studies activity were by whole-cell planar patch-clamp...
TRPC5 is a mammalian homologue of the Drosophila Transient Receptor Potential (TRP) channel and has expression functions in cardiovascular nervous systems. It forms calcium-permeable cation that can be activated by variety signals including carbachol (acting at muscarinic receptors), lanthanides (e.g. Gd3+) phospholipids lysophosphatidylcholine: LPC). Here we report effects inhalational (halothane chloroform) intravenous (propofol) general anaesthetics upon TRPC5.Human channels were...