A5101897999- Ion Channels and Receptors
- Genetic and phenotypic traits in livestock
- Animal Genetics and Reproduction
- Genetic Mapping and Diversity in Plants and Animals
- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Meat and Animal Product Quality
- Cancer-related molecular mechanisms research
- Peroxisome Proliferator-Activated Receptors
- CRISPR and Genetic Engineering
- Antimicrobial Peptides and Activities
- Milk Quality and Mastitis in Dairy Cows
- Thyroid Cancer Diagnosis and Treatment
- Immune Response and Inflammation
- Lipid metabolism and biosynthesis
- Cell death mechanisms and regulation
- Calpain Protease Function and Regulation
- Cardiac electrophysiology and arrhythmias
- Animal Nutrition and Physiology
- Phytochemicals and Antioxidant Activities
- Growth Hormone and Insulin-like Growth Factors
- RNA and protein synthesis mechanisms
- Herbal Medicine Research Studies
- NF-κB Signaling Pathways
- Crop Yield and Soil Fertility
Xuzhou Medical College
2020-2025
University of Hull
2012-2024
Hull York Medical School
2012-2024
Children's Hospital of Fudan University
2024
Second Affiliated Hospital of Xi'an Jiaotong University
2011-2017
Chinese Academy of Agricultural Sciences
2006-2016
Zhongshan Hospital of Xiamen University
2016
Xi'an Jiaotong University
2014-2015
Institute of Animal Sciences
1993-2014
Ministry of Agriculture
2013
Mammalian counterparts of the Drosophila trp gene have been suggested to encode store-operated Ca(2+) channels. These specialized channels are widely distributed and may a general function reload into sarcoplasmic reticulum as well specific functions, including control cell proliferation muscle contraction. Heterologous expression mammalian genes enhances or generates channel activity, but crucial question whether any native subunits remains unanswered. We investigated if TrpC1 protein...
TRPM2 is a member of the melastatin-related TRP (transient receptor potential) subfamily. It expressed in brain and lymphocytes forms cation channel that activated by intracellular ADP-ribose associated with cell death. In this study we investigated calcium dependence human under tetracycline-dependent promoter HEK-293 cells. expression was enhanced hydrogen peroxide-evoked signals. whole-cell patch clamp recordings, switching from barium- to calcium-containing extracellular solution...
1 2-aminoethoxydiphenyl borate (2-APB) has been widely used to examine the roles of inositol 1,4,5-trisphosphate receptors (IP3Rs) and store-operated Ca2+ entry is an emerging modulator cationic channels encoded by transient receptor potential (TRP) genes. 2 Using Ca2+-indicator dye patch-clamp recording we first examined blocking effect 2-APB on human TRPC5 expressed in HEK-293 cells. 3 The concentration-response curve IC50 20 microM slope close 1.0, suggesting one molecule binds per...
Occlusive vascular disease is a widespread abnormality leading to lethal or debilitating outcomes such as myocardial infarction and stroke. It part of atherosclerosis evoked by clinical procedures including angioplasty grafting saphenous vein in bypass surgery. A causative factor the switch smooth muscle cells an invasive proliferative mode, neointimal hyperplasia. Here we reveal importance this process TRPC1, homolog Drosophila transient receptor potential. Using 2 different vivo models...
The reactivity of the vascular wall to endothelin-1 (ET-1) is influenced by cholesterol, which possible importance for progression atherosclerosis. To elucidate signaling steps affected, cholesterol acceptor methyl-β-cyclodextrin (mβcd, 10 mmol/L) was used manipulate membrane and disrupt caveolae in intact rat arteries. In endothelium-denuded caudal artery, contractile responsiveness nmol/L ET-1 (mediated ET A receptor) reduced mβcd increased cholesterol. Neither ligand binding nor...
Loss of the smooth muscle contractile phenotype is critical in atherosclerosis and restenosis after angioplasty, but its early signals are incompletely understood. In this study, we have explored role transient receptor potential canonical (TRPC) proteins, which been suggested to mediate store-operated Ca2+ entry (SOCE). Contractility rat cerebral arteries organ culture preserved for several days, whereas SOCE increased. correlation with increase that nifedipine-insensitive whole cell...
In a screen of potential lipid regulators transient receptor (TRP) channels, we identified sphingosine-1-phosphate (S1P) as an activator TRPC5. We explored the relevance to vascular biology because S1P is key cardiovascular signaling molecule. TRPC5 expressed in smooth muscle cells human vein along with TRPC1, which forms complex Importantly, also activates TRPC5-TRPC1 heteromultimeric channel. Because TRPC channels are linked neuronal growth cone extension, considered related concept for...
Here we explore the activation mechanisms of human TRPC5, a putative cationic channel that was cloned from region X chromosome associated with mental retardation. No basal activity evident but induced by carbachol stimulation muscarinic receptors independently Ca2+ release. This is 'receptor activation', as described for mouse TRPC5. In addition, and in absence receptor stimulation, extracellular gadolinium (0.1 mm) activated an effect mimicked 5-20 mm intracellular buffered. We refer to...
Background Electrocoagulation and laser evaporation for urethral condylomata acuminata have high recurrence rates can be associated with malformations. Objectives To investigate the effect of photodynamic therapy (PDT) topical 5‐aminolaevulinic acid (ALA) on to examine histological changes in lesions after ALA‐PDT. Methods Patients (n = 164) were given ALA followed by intraurethral PDT through a cylindrical fibre. included 11 individuals 16 penile or vulval condylomatous which biopsied...
TRPC calcium channels are emerging as a ubiquitous feature of vertebrate cells, but understanding them is hampered by limited knowledge the mechanisms activation and identity endogenous regulators. We have revealed that one channels, TRPC5, strongly activated common lysophospholipids including lysophosphatidylcholine (LPC) but, contrast, not arachidonic acid. Although TRPC5 was stimulated agonists at G-protein-coupled receptors, LPC occurred downstream independently G-protein signaling. The...
The canonical transient receptor potential (TRPC) channels are Ca2+-permeable cationic controlling the Ca2+ influx evoked by G protein-coupled activation and/or store depletion. Here we investigate involvement of TRPCs in cell differentiation lung cancer. expression and correlation to cancer grade non-small (NSCLC) were analyzed real-time PCR immunostaining using tissue microarrays from 28 patient samples. association with was also investigated line A549 Western blotting. channel activity...
Significance Statement Mutations in the transient receptor potential channel 6 ( TRPC6 ) gene are associated with an inherited form of FSGS. Emerging evidence has linked activity calpain activation and podocyte injury. In this study, authors generated a knockout cell line from mice, engineering these cells to express wild-type various mutations TRPC6. They show that binds both ERK 1/2 calpain, is important for localization membrane, independent calcium influx. This interaction vital motility...
Elevated reactive oxygen species (ROS) in type 2 diabetes cause cellular damage many organs. Recently, the new class of glucose-lowering agents, SGLT-2 inhibitors, have been shown to reduce risk developing diabetic complications; however, mechanisms such beneficial effect are largely unknown. Here we aimed investigate effects dapagliflozin on cell proliferation and death under oxidative stress conditions explore its underlying mechanisms. Human proximal tubular cells (HK-2) were used. Cell...
The calcium- and sodium-permeable transient receptor potential channel TRPC5 has an inhibitory role in neuronal outgrowth but the mechanisms governing its activity are poorly understood. Here we propose a mechanism involving calcium sensor-1 (NCS-1) protein. Inhibitory mutants of NCS-1 enhance neurite similarly. Mutant does not inhibit surface-expression generally suppresses activity, irrespective whether it is evoked by carbachol, store depletion, lanthanides or elevated intracellular...
Smooth muscle cells in arterioles have pivotal roles the determination of blood pressure and distribution local flow. The exhibit calcium entry response to passive store depletion, but mechanisms relevance this phenomenon are poorly understood. Previously, a role for canonical transient receptor potential 1 (TRPC1) was indicated, heterologous expression studies showed TRPC1 poor function isolation, suggesting requirement additional proteins. Here we test hypothesis that TRPC5 is such an...
TRPC channels are Ca²⁺-permeable cationic controlling Ca²⁺ influx response to the activation of G protein-coupled receptors and protein tyrosine kinase pathways or depletion stores. Here we aimed investigate whether can act as potential therapeutic targets for ovarian cancer. The mRNAs TRPC1, TRPC3, TRPC4 TRPC6 were detected in human adenocarcinoma. spliced variants TRPC1β, TRPC3a, TRPC4β, TRPC4γ, with exon 3 4 deletion highly expressed cancer cells, a novel isoform TRPC1 9 (TRPC1(E9del))...
Abstract Impaired albumin reabsorption by proximal tubular epithelial cells (PTECs) has been highlighted in diabetic nephropathy (DN), but little is known about the underlying molecular mechanisms. Here we find that ORAI1-3, are preferentially expressed PTECs and downregulated patients with DN. Hyperglycemia or blockade of insulin signaling reduces expression ORAI1-3. Inhibition ORAI channels BTP2 diethylstilbestrol silencing impairs uptake. Transgenic mice expressing a dominant-negative...
<title>Abstract</title> Introduction: The care needs of older adults with mild dementia have not been studied in depth. In this study, we investigated the impairment status patients and analyzed different levels terms activities daily living, medical services, environmental care, psychological comfort social participation, factors influencing them. Methods This study used a cross-sectional research method to investigate mildly demented aged 60 years above Chinese community. A...
1. In this study, we determined a pharmacological profile of store-operated channels (SOCs) in smooth muscle cells rabbit pial arterioles. Ca(2+)-indicator dyes, fura-PE3 or fluo-4, were used to track [Ca(2+)](i) and 10 micro M methoxyverapamil (D600) was present all experiments on SOCs prevent voltage-dependent Ca(2+) entry. Store depletion induced using thapsigargin cyclopiazonic acid. 2. SOC-mediated entry inhibited concentration dependently by Gd(3+) (IC(50) 101 nM). It also La(3+) (70%...