A5077078067- Mesenchymal stem cell research
- Rheumatoid Arthritis Research and Therapies
- Osteoarthritis Treatment and Mechanisms
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cancer survivorship and care
- Ion Channels and Receptors
- Cytokine Signaling Pathways and Interactions
- Palliative Care and End-of-Life Issues
- Respiratory and Cough-Related Research
- Bone and Joint Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- SARS-CoV-2 and COVID-19 Research
- Systemic Lupus Erythematosus Research
- Influenza Virus Research Studies
- Bone fractures and treatments
- Neonatal Respiratory Health Research
- Musculoskeletal synovial abnormalities and treatments
- Ion channel regulation and function
- Lung Cancer Diagnosis and Treatment
- Cancer Cells and Metastasis
- Periodontal Regeneration and Treatments
- COVID-19 epidemiological studies
- Health, psychology, and well-being
- Toxoplasma gondii Research Studies
- Fibromyalgia and Chronic Fatigue Syndrome Research
Humber Teaching NHS Foundation Trust
2019-2025
Purdue University West Lafayette
2024
Trinity House
2008-2019
University of Leeds
2000-2013
NIHR Leeds Musculoskeletal Biomedical Research Unit
2003-2013
Chapel Allerton Hospital
2007-2011
Lister Hospital
2011
East Riding of Yorkshire Council
2010
University of Sheffield
2008
University of Liverpool
2008
Abstract Objective There is an increased interest in rheumatology mesenchymal progenitor/stem cells (MPCs) and their roles rheumatic diseases, but little known about the phenotype of these vivo. The aim this study was to isolate characterize human bone marrow (BM) MPCs. Methods Fluorescence microscopy used identify putative MPCs among adherent BM cells. To purify them, a positive selection with antifibroblast microbeads used, combined fluorescence‐activated cell sorting (FACS) for...
Abstract Objective To evaluate synovial fluid (SF) for the presence of mesenchymal progenitor cells (MPCs), to compare SF MPCs with bone marrow (BM) MPCs, and enumerate these in both inflammatory arthritis osteoarthritis (OA). Methods from 100 patients (53 rheumatoid [RA], 20 OA, 27 other arthropathies) was evaluated. establish multipotentiality, polyclonal single cell–derived cultures fibroblasts were examined by standard quantitative differentiation assays. Their phenotype before after...
Abstract Objective Arthritic synovial fluid (SF) contains mesenchymal stem cells (MSCs), which could simply reflect their shedding from diseased joint structures. This study used the bovine model to explore SF MSCs in health and enumerated them at earliest stages of human osteoarthritis (OA) radiographically normal joints. Methods Clonogenicity multipotentiality were compared with donor‐matched bone marrow (BM) single‐cell level. The colony‐forming unit–fibroblastic assay was for MSC...
Abstract Background: To study the biology of rare bone marrow (BM) multipotent mesenchymal stromal cells (MSCs), recognized protocols are needed. Colony‐forming unit‐fibroblast (CFU‐F) assays have historically been used for enumeration MSCs. However, need to isolate and further analyze MSCs requires new strategies based on cell surface markers. The purpose this work was verify phenotype BM in vivo develop flow cytometry‐based methods their evaluation. Methods: Pre‐enrichment with...
To test the hypothesis that CD45(low)CD271+ bone marrow multipotential stromal cells (MSCs) are abundant in trabecular niche and to explore their functional "fitness" health osteoarthritis (OA).Following enzymatic extraction, MSC release was evaluated using colony-forming unit-fibroblast (CFU-F) unit-osteoblast assays, flow cytometry, confocal microscopy. isolated by fluorescence-activated cell sorting were enumerated expanded under standard clonal conditions. Their proliferative osteogenic...
<h3>Objectives:</h3> Abatacept is the only agent currently approved to treat rheumatoid arthritis (RA) that targets co-stimulatory signal required for full T-cell activation. No studies have been conducted on its effect synovium, primary site of pathology. The aim this study was determine synovial abatacept in patients with RA and an inadequate response tumour necrosis factor alpha (TNFα) blocking therapy. <h3>Methods:</h3> This first mechanistic incorporated both dynamic contrast-enhanced...
The utility of autologous chondrocytes for cartilage repair strategies in older subjects with osteoarthritis (OA) may be limited by both age-related and disease-associated decline chondrogenesis. aim this work was to assess OA Hoffa's fat pad as an alternative source chondroprogenitor cells compare it derived from different areas cartilage.Cartilage tissue digests were obtained 26 knee compared normal bone marrow (BM) mesenchymal stem (MSCs) respect their vitro colony-forming potential,...
Achieving joint regeneration in rheumatoid arthritis (RA) represents a future challenge. Autologous synovial mesenchymal stem cells (MSCs) could be therapeutically exploited. However, the inflammatory milieu RA synovium adversely affect endogenous MSC function. To test this hypothesis, frequency and multipotency of MSCs was evaluated relation to existing inflammation.Synovial inflammation measured using arthroscopic visual analogue score (VAS) further validated immunohistochemistry flow...
In the 1997-98 academic year, we conducted a longitudinal study of meningococcal carriage and acquisition among first-year students at Nottingham University, Nottingham, United Kingdom. We examined dynamics long-term with detailed characterization isolates. Pharyngeal swabs were obtained from 2,453 start year (October), later on during autumn term, again in March. Swabs immediately cultured selective media, meningococci identified serologically characterized. Nongroupable strains genetically...
Background aimsMesenchymal stromal cells (MSCs) are regenerative and immuno-privileged that used for both tissue regeneration treatment of severe inflammation-related disease. For quality control manufactured MSC batches in regard to mature fat cell contamination, a quantitative method measuring adipogenesis is needed.MethodsFour previously proposed methods were validated with the use bone marrow (BM) MSCs during 21-day vitro assay. Oil red staining was scored semiquantitatively; peroxisome...
About 90 % of patients with intra-thoracic malignancy experience breathlessness. Breathing training is helpful, but it unknown whether repeated sessions are needed. The present study aims to test three better than one for breathlessness in this population. This a multi-centre randomised controlled non-blinded parallel arm trial. Participants were allocated or single (1:2 ratio) using central computer-generated block randomisation by an independent Trials Unit and stratified centre. setting...
Introduction Breathlessness is common and impairs the quality of life people with idiopathic pulmonary fibrosis (IPF) non-IPF fibrotic interstitial lung diseases (ILD). We report findings a multicentre, fast-track (wait-list), mixed-methods, randomised controlled, feasibility study complex breathlessness intervention in breathless IPF ILD patients. Methods Breathless patients were to receive within 1 week (fast-track) or after 8 weeks (wait-list). The comprised two face-to-face one telephone...
The non-steroidal anti-inflammatory drugs (NSAIDs) are widely used for analgesia but may inhibit bone formation. We investigated whether the reported NSAID effect on is related to inhibition of marrow mesenchymal stem cell (MSC) proliferation and osteogenic chondrogenic differentiation evaluated both cyclooxygenase (COX)-1 COX-2 specific drugs. effects seven COX-1 inhibitors MSC were tested using Vybrant, sodium 3′-[1-(phenylaminocarbonyl)- 3,4-tetrazolium]-bis (4-methoxy-6-nitro) benzene...
Calcium-permeable channels are known to have roles in many mammalian cell types but the expression and contribution of such ion synovial cells is mostly unknown. The objective this study was investigate potential relevance Transient Receptor Potential Melastatin 3 (TRPM3) channel fibroblast-like synoviocytes (FLSs) patients with rheumatoid arthritis.The used RT-PCR immunofluorescence detect mRNA protein. Intracellular calcium measurement detected activity a FLS cell-line primary cultures...
An abnormal CD4+ T cell subset related to inflammation exposure (inflammation-related cells, IRC) has been identified in rheumatoid arthritis (RA). Patients with inflammatory and non-inflammatory diseases were used examine the relationship between this vivo.Blood was collected from healthy controls patients RA (active disease or clinical remission), Crohn's osteoarthritis. IRC chemokine receptors quantified by flow cytometry. Thymic activity apoptotic factors measured real-time polymerase...
Objectives. Clinical response to TNF-α blockade in the treatment of RA is heterogeneous. The study aims were determine whether pre-treatment synovial cytokine expression predicted infliximab and changes after therapy correlated with response.
Aim: To enumerate and characterize mesenchymal stem cells (MSCs) endothelial (ECs) in umbilical cord (UC) tissue digests. Materials & methods: Cultured UC were characterized phenotypically, functionally by using 48-gene arrays. Native MSCs ECs enumerated flow cytometry. Results: Compared with bone marrow (BM) MSCs, displayed significantly lower (range 4–240-fold) basal levels of bone-related transcripts, but their phenotypes similar (CD73+, CD105+, CD90+, CD45- CD31-). responded well to...
The ability of normal mouse mammary epithelial cells (MECs) to express insulin-like growth factor-binding proteins (IGFBPs) was examined. MECs were isolated from day 11 pregnant mice and cultured on floating collagen gels in serum-free basal medium. After 24 h, the medium replaced with fresh with/or without PRL (mPRL), placental lactogen-I (mPL-I), mPL-II, GH (mGH), IGF-I, IGF-II, either alone or combinations. for an additional 5 days before collection conditioned (CM). relative amount...
Introduction Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive lung disease that causes breathlessness cough worsen over time, limiting daily activities negatively impacting quality of life. Although treatments are now available slow the rate function decline, trials these have failed to show improvement in symptoms or There an immediate unmet need for evidenced-based interventions improve patients' symptom burden make difference everyday living. This study aims assess...
Background IL-21 is secreted by activated T cells and modulates immune cell functions with both proinflammatory anti-inflammatory effects.IL-21 receptor (IL-21R), homologous to IL-2Rβ IL-4Rα, associates the gamma common chain upon ligand binding.It was recently described that IL-21R overexpressed in inflamed synovial membrane on leucocytes of rheumatoid arthritis patients.Objective Previously we have shown blockade pathway soluble IL-21R-Fc resulted a reduction clinical signs rodent...