A5070366885- Mesenchymal stem cell research
- Extracellular vesicles in disease
- Tissue Engineering and Regenerative Medicine
- Osteoarthritis Treatment and Mechanisms
- Bone fractures and treatments
- Tendon Structure and Treatment
- Knee injuries and reconstruction techniques
- Cancer Cells and Metastasis
- Bone and Joint Diseases
- Electrospun Nanofibers in Biomedical Applications
- Reproductive System and Pregnancy
- Fibroblast Growth Factor Research
- 3D Printing in Biomedical Research
- Diabetic Foot Ulcer Assessment and Management
- Orthopedic Infections and Treatments
- Bone Tissue Engineering Materials
- Total Knee Arthroplasty Outcomes
- Lower Extremity Biomechanics and Pathologies
- Immune cells in cancer
- Antifungal resistance and susceptibility
- Sarcoma Diagnosis and Treatment
- Congenital Diaphragmatic Hernia Studies
- Shoulder Injury and Treatment
- Periodontal Regeneration and Treatments
- Neonatal Respiratory Health Research
University of Miami Health System
2018-2025
University of Miami
2018-2025
Aristotle University of Thessaloniki
2014-2025
Hippocration General Hospital
2023-2024
Miami Transplant Institute
2019-2021
Foundation for Research and Technology Hellas
2014-2020
Institute of Molecular Biology and Biotechnology
2014-2018
Biomedical Research Institute
2018
University of Ioannina
2014-2017
NIHR Leeds Musculoskeletal Biomedical Research Unit
2011-2013
Acute respiratory distress syndrome (ARDS) in COVID-19 is associated with high mortality. Mesenchymal stem cells are known to exert immunomodulatory and anti-inflammatory effects could yield beneficial ARDS. The objective of this study was determine safety explore efficacy umbilical cord mesenchymal cell (UC-MSC) infusions subjects A double-blind, phase 1/2a, randomized, controlled trial performed. Randomization stratification by ARDS severity used foster balance among groups. All were...
To test the hypothesis that CD45(low)CD271+ bone marrow multipotential stromal cells (MSCs) are abundant in trabecular niche and to explore their functional "fitness" health osteoarthritis (OA).Following enzymatic extraction, MSC release was evaluated using colony-forming unit-fibroblast (CFU-F) unit-osteoblast assays, flow cytometry, confocal microscopy. isolated by fluorescence-activated cell sorting were enumerated expanded under standard clonal conditions. Their proliferative osteogenic...
Abstract Objective Controversy surrounds the identity and functionality of rare bone marrow–derived multipotential stromal cells (BM‐MSCs), including their differentiation capabilities, relationship to pericytes hematopoiesis‐supporting cells, relevance culture‐expanded progeny in studies skeletal biology development cell‐based therapies. The aim this study was clarify nature candidate BM‐MSCs by profiling transcripts that reflect different aspects putative functions vivo. Methods Rare,...
Abstract CD146+ bone marrow-derived mesenchymal stem/stromal cells (BM-MSCs) play key roles in the perivascular niche, skeletogenesis, and hematopoietic support; however, comprehensive evaluation of therapeutic potency has yet to be determined. In this study, vitro inflammatory priming crude human BM-MSCs (n = 8) captured a baseline signature responses, including enriched with coexpression CD107aHigh, CXCR4High, LepRHigh, transcriptional profile, enhanced secretory capacity, robust...
osteoarthritic human articular cartilage (AC)-derived cells (CCs) with same-donor bone marrow (BMSCs) and adipose tissue (ASCs)-derived mesenchymal stem were compared, in terms of stemness features, secretory immunomodulatory responses to inflammation.proteoglycan 4 (PRG4) presence was evaluated AC CCs. MSCs CCs (n = 8) cultured (P1 P4) characterized for clonogenicity, nanog homeobox (NANOG), POU class 5 1 (POU5F1) expression, immunotypification, tri-lineage differentiation. Their basal...
During the progression of knee osteoarthritis (OA), synovium and infrapatellar fat pad (IFP) can serve as source for Substance P (SP) calcitonin gene-related peptide (CGRP), two important pain-transmitting, immune, inflammation modulating neuropeptides. Our previous studies showed that pad-derived mesenchymal stem/stromal cells (MSC) acquire a potent immunomodulatory phenotype actively degrade via CD10 both in vitro vivo. On this basis, our hypothesis is CD10-bound IFP-MSC sEVs be engineered...
Background aimsMesenchymal stromal cells (MSCs) are regenerative and immuno-privileged that used for both tissue regeneration treatment of severe inflammation-related disease. For quality control manufactured MSC batches in regard to mature fat cell contamination, a quantitative method measuring adipogenesis is needed.MethodsFour previously proposed methods were validated with the use bone marrow (BM) MSCs during 21-day vitro assay. Oil red staining was scored semiquantitatively; peroxisome...
The infrapatellar fat pad (IFP) serves as a reservoir of Mesenchymal Stem Cells (MSC), and with adjacent synovium plays key roles in joint disease including the production Substance P (SP) affecting local inflammatory responses transmitting nociceptive signals. Here, we interrogate human IFP-derived MSC (IFP-MSC) reaction to pro-fibrotic environments (cell priming by TNFα/IFNγ TNFα/IFNγ/CTGF exposure respectively), compared bone marrow-derived (BM-MSC). Naïve IFP-MSC exhibit increased...
The perivascular localization of endometrial mesenchymal stem/stromal cells (eMSC) allows them to sense local and distant tissue damage, promoting repair healing. Our hypothesis is that eMSC therapeutic effects are largely exerted via their exosomal secretome (eMSC EXOs) by targeting the immune system angiogenic modulation. For this purpose, EXOs isolated from Crude CD146+ populations were compared for miRNA signatures immunomodulatory functionality under inflammatory conditions. profiling...
Abstract Within the human knee infrapatellar fat pad (IFP) and synovium, resident synoviocytes macrophages contribute to onset progression of inflammatory joint diseases. Our hypothesis is that IFP-derived mesenchymal stem cells (IFP-MSC) robust immunomodulatory therapeutic effects are largely exerted via their exosomal (IFP-MSC EXOs) secretome by attenuating pro-inflammatory activation. IFP-MSC EXOs showed distinct miRNA protein profiles. Reactome analysis 24 miRNAs highly present in...
The onset and progression of human inflammatory joint diseases are strongly associated with the activation resident synovium/infrapatellar fat pad (IFP) pro-inflammatory pain-transmitting signaling. We recently reported that intra-articularly injected IFP-derived mesenchymal stem/stromal cells (IFP-MSC) acquire a potent immunomodulatory phenotype actively degrade substance P (SP) via neutral endopeptidase CD10 (neprilysin). Our hypothesis is IFP-MSC robust therapeutic effects largely exerted...
Introduction: The epidemiology of candidemia has shifted in the past few decades; drug-resistant non-albicans Candida species have become more prevalent worldwide. aim this retrospective study was to determine isolated from hospitalized neonates, children and adults, investigate a potential changing susceptibility pattern large general tertiary hospital. Methods: All unique strains cases between 1 January 2020 15 October 2024 were identified, their profile characterized. distribution...
We aimed at explaining the mechanism of therapeutic effect Umbilical Cord Mesenchymal Stem Cells (UC-MSC) in subjects with COVID-19 Acute Respiratory Distress Syndrome (ARDS). Patients ARDS present a hyperinflammatory response characterized by high levels circulating pro-inflammatory mediators, including tumor necrosis factor α and β (TNFα TNFβ). Inflammatory functions these TNFs can be inhibited soluble TNF Receptor 2 (sTNFR2). In patients ARDS, UC-MSC appear to impart robust...
Synovitis and infrapatellar fat pad (IFP) fibrosis participate in various conditions of the knee. Substance P (SP), a neurotransmitter secreted within those structures historically associated with nociception, also modulates local neurogenic inflammatory fibrotic responses. Exposure IFP mesenchymal stem cells (IFP-MSCs) to proinflammatory/profibrotic environment (ex vivo priming TNFα, IFNγ, CTGF) induces their expression CD10/neprilysin, effectively degrading SP vitro vivo.The purpose was...
Diffusion is a limiting factor in regenerating large tissues (100-200 μm) due to reduced nutrient supply and waste removal leading low viability of the cells as neovascularization implant by host slow process. Thus, generating prevascularized tissue engineered constructs, which endothelial (ECs) mural (MCs) cells, such smooth muscle (SMCs), pericytes (PCs), are preassembled into functional vitro vessels capable rapidly connecting vasculature could overcome this obstacle. Toward purpose,...