A5008060564- Pancreatic function and diabetes
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Liver physiology and pathology
- Pluripotent Stem Cells Research
- Diabetes and associated disorders
- Diabetes Management and Research
- Extracellular vesicles in disease
- Congenital Diaphragmatic Hernia Studies
- Pediatric Hepatobiliary Diseases and Treatments
- Organ and Tissue Transplantation Research
- Genetics and Neurodevelopmental Disorders
- Cancer Cells and Metastasis
- Apelin-related biomedical research
- Pancreatic and Hepatic Oncology Research
- Reproductive System and Pregnancy
- Organ Transplantation Techniques and Outcomes
- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Diet, Metabolism, and Disease
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Diet and metabolism studies
- Hematopoietic Stem Cell Transplantation
- Congenital Anomalies and Fetal Surgery
University of Miami
2015-2025
Dr. John T. Macdonald Foundation
2021
Miami Transplant Institute
2017-2021
University of Bologna
2007-2014
Consorzio Interuniversitario Nazionale per l'Informatica
2009
Abstract Background Term Amniotic membrane (AM) is a very attractive source of Mesenchymal Stem Cells (MSCs) due to the fact that this fetal tissue usually discarded without ethical conflicts, leading high efficiency in MSC recovery with no intrusive procedures. Here we confirmed term AM, as previously reported literature, an abundant hMSCs; particular further investigated AM differentiation potential by assessing whether these cells may also be committed angiogenic fate. In agreement...
Acute respiratory distress syndrome (ARDS) in COVID-19 is associated with high mortality. Mesenchymal stem cells are known to exert immunomodulatory and anti-inflammatory effects could yield beneficial ARDS. The objective of this study was determine safety explore efficacy umbilical cord mesenchymal cell (UC-MSC) infusions subjects A double-blind, phase 1/2a, randomized, controlled trial performed. Randomization stratification by ARDS severity used foster balance among groups. All were...
We have described multipotent progenitor-like cells within the major pancreatic ducts (MPDs) of human pancreas. They express PDX1, its surrogate surface marker P2RY1, and bone morphogenetic protein (BMP) receptor 1A (BMPR1A)/activin-like kinase 3 (ALK3), but not carbonic anhydrase II (CAII). Here we report single-cell RNA sequencing (scRNA-seq) ALK3bright+-sorted ductal cells, a fraction that harbors BMP-responsive cells. Our analysis unveiled existence multiple subpopulations along two...
Peribiliary glands (PBGs) in bile duct walls, and pancreatic (PDGs) associated with ducts, humans of all ages, contain a continuous, ramifying network cells overlapping maturational lineages. We show that proximal (PBGs)-to-distal lineages start near the duodenum expressing markers pluripotency (NANOG, OCT4, SOX2), proliferation (Ki67), self-replication (SALL4), early hepato-pancreatic commitment (SOX9, SOX17, PDX1, LGR5), transitioning to PDG no expression or markers, maintenance genes...
Dental pulp is a heterogeneous microenviroment where unipotent progenitor and pluripotent mesenchymal stem cells cohabit. In this study we investigated whether human Pulp Stromal (Stem) Cells (DP-SCs) committed to the angiogenic fate. DP-SCs showed specific immunophenotypical profile positive for CD29, CD44, CD73, CD105, CD166 negative CD14, CD34, CD45, in accordance with that reported bone marrow-derived SCs. The Oct-4 expression DP-SCs, evaluated through RT-PCR analysis, increased relation...
The exocrine pancreas can give rise to endocrine insulin-producing cells upon ectopic expression of key transcription factors. However, the need for genetic manipulation remains a translational hurdle diabetes therapy. Here we report conversion adult human nonendocrine pancreatic tissue into cell types by exposure bone morphogenetic protein 7. use this U.S. Food and Drug Administration–approved agent, without any manipulation, results in neogenesis clusters that exhibit high insulin content...
Abstract Biologic and clinical interest in human mesenchymal stromal cells (hMSC) has risen over the last years, mainly due to their immunosuppressive properties. In this study, we investigated basis of immunomodulant possible variability using hMSC from different sources (amniotic membrane, chorion, bone marrow either healthy subjects or patients with hematological malignancies, HM) having discordant positivity for several immunological markers. The CD90+ reduced lymphoproliferative...
Treatment of human pancreatic non-endocrine tissue with Bone Morphogenetic Protein 7 (BMP-7) leads to the formation glucose-responsive β-like cells. Here, we show that BMP-7 acts on extrainsular cells expressing PDX1 and BMP receptor activin-like kinase 3 (ALK3/BMPR1A). In vitro lineage tracing indicates ALK3+ cell populations are multipotent. PDX1+/ALK3+ absent from islets but prominently represented in major ducts duct glands. We identified purinergic P2Y1 (P2RY1) as a surrogate surface...
Fetal membranes (FM) derived mesenchymal stromal/stem cells (MSCs) are higher in number, expansion and differentiation abilities compared with those obtained from adult tissues, including bone marrow. Upon systemic administration, ex vivo expanded FM-MSCs preferentially home to damaged tissues promoting regenerative processes through their unique biological properties. These characteristics together immune-privileged nature immune suppressive activity, a low infection rate young age of...
We aimed at explaining the mechanism of therapeutic effect Umbilical Cord Mesenchymal Stem Cells (UC-MSC) in subjects with COVID-19 Acute Respiratory Distress Syndrome (ARDS). Patients ARDS present a hyperinflammatory response characterized by high levels circulating pro-inflammatory mediators, including tumor necrosis factor α and β (TNFα TNFβ). Inflammatory functions these TNFs can be inhibited soluble TNF Receptor 2 (sTNFR2). In patients ARDS, UC-MSC appear to impart robust...
Recurrence of autoimmunity and allograft rejection represent major challenges that impact the success islet transplantation. Despite remarkable improvements achieved in immunosuppression strategies after publication Edmonton protocol, long-term data intra-hepatic transplantation show a gradual decline beta-cell function. Therefore, there is growing interest investigation novel, safe effective anti-inflammatory immunomodulatory able to promote graft survival notable clinical outcomes...
To test 25-hydroxy vitamin D (25(OH)D) levels among subjects with new-onset type 1 diabetes (T1D) and their association fasting stimulated C-peptide at study entry in an open-label randomized trial. We conducted a post hoc secondary analysis of the POSEIDON trial (a Pilot, Safety Feasibility Trial High-Dose Omega-3 fatty acids Cholecalciferol Supplementation Type Diabetes). Eligibility criteria included age 6 to 65 years, T1D up 10 years duration, presence least islet autoantibody, ≥0.066...