A5046180673- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- Immunodeficiency and Autoimmune Disorders
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Acute Lymphoblastic Leukemia research
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Mesenchymal stem cell research
- Blood disorders and treatments
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Sarcoma Diagnosis and Treatment
- Erythrocyte Function and Pathophysiology
- Neonatal Respiratory Health Research
- Viral-associated cancers and disorders
- RNA modifications and cancer
- DNA Repair Mechanisms
- Cellular Mechanics and Interactions
Stanford University
2016-2025
Stanford Medicine
2008-2024
Stanford Health Care
2024
Cancer Institute (WIA)
2024
Stanford Cancer Institute
2024
Lucile Packard Children's Hospital
2006-2022
Palo Alto University
2008-2022
University Hospital of Zurich
2019
Pediatrics and Genetics
2012-2013
Children's Hospital of Los Angeles
2000-2011
In T cell-deficient conditions, naive cells undergo spontaneous "homeostatic" proliferation in response to contact with self-MHC/peptide ligands. With the aid of an vitro system, we show here that homeostatic is also cytokine-dependent. The cytokines IL-4, IL-7, and IL-15 enhanced vitro. Of these cytokines, only IL-7 was found be critical; thus, underwent IL-4(-) IL-15(-) hosts but proliferated minimally IL-7(-) hosts. addition proliferation, prolonged survival requires IL-7. Thus, naïve...
Why some patients with seizures are successfully treated antiepileptic drugs (AEDs) and others prove medically intractable is not known. Inadequate intraparenchymal drug concentration a possible mechanism of resistance to AEDs. The multiple gene (MDR1) encodes P-glycoprotein, an energy-dependent efflux pump that exports planar hydrophobic molecules from the cell. If P-glycoprotein expressed in brain epilepsy AEDs exported by lower concentrations could contribute lack response such patients....
Although the mammalian immune system is generally thought to develop in a linear fashion, findings avian and murine species argue instead for developmentally ordered appearance (or "layering") of distinct hematopoietic stem cells (HSCs) that give rise lymphocyte lineages at different stages development. Here we provide evidence an analogous layered humans. Our results suggest fetal adult T are populations arise from HSCs present We also cell lineage biased toward tolerance. These...
In a previous investigation, we demonstrated that mesenchymal stem cells (MSCs) actively migrated to cardiac allografts and contributed graft fibrosis and, lesser extent, myocardial regeneration. The cellular/molecular mechanism responsible for MSC migration, however, is poorly understood. This paper examines the role of CD44-hyaluronan interaction in using rat line Ap8c3 mouse CD44-/- or CD44+/+ bone marrow stromal (BMSCs). Platelet-derived growth factor (PDGF) stimulation significantly...
The immunological mechanisms required to engender resistance have been defined in few infectious diseases of man, and the role specific cytokines is unclear. Leprosy presents clinically as a spectrum which correlates with cell-mediated immunity pathogen. To assess situ cytokine patterns, messenger RNA extracted from leprosy skin biopsy specimens was amplified by polymerase chain reaction 14 cytokine-specific primers. In lesions resistant form disease, RNAs coding for interleukin-2...
Abstract Gene correction in human long-term hematopoietic stem cells (LT-HSCs) could be an effective therapy for monogenic diseases of the blood and immune system. Here we describe approach X-linked sSevere cCombined iImmunodeficiency (SCID-X1) using targeted integration a cDNA into endogenous start codon to functionally correct disease-causing mutations throughout gene. Using CRISPR-Cas9/AAV6 based strategy, achieve up 20% frequencies LT-HSCs. As measures lack toxicity observe no evidence...
Infection by murine retroviruses in embryonic carcinoma (EC) and stem cells is highly restricted. The transcriptional unit of the Moloney leukemic virus (MoMuLV) long terminal repeat (LTR) inactive EC association with increased proviral methylation. In this study, expression F9 was achieved from novel retroviral vectors containing three modifications MoMuLV-based vector: presence myeloproliferative sarcoma LTR, substitution primer binding site, either deletion a negative control region at 5'...
The common cytokine receptor γ chain (γc), an essential component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15, is critical development function lymphocytes. Recently, a novel lymphokine (IL-21) its (IL-21Rα) were described which profoundly affect growth activation state B, T, NK cells in concert with other lymphokines or stimuli [Parrish-Novak, J., et al. (2000) Nature 408, 57−63]. In this report, we show that γc also required signaling IL-21 (IL-21R) using γc-deficient X-linked...
Abstract Orthotopic brain tumor growth is inhibited in athymic mice by the daily systemic administration of α v ‐integrin antagonist EMD 121974. This compound, a cyclic RGD‐penta‐peptide, potent inhibitor angiogenesis, which induces apoptosis growing endothelial cells through inhibition their interaction with matrix proteins vitronectin and tenascin. Here we show that 121974 also ‐integrin‐expressing cell lines U87 MG DAOY detaching them from tenascin, known to be essential for invasion....
Duchenne muscular dystrophy (DMD) is a severe progressive muscle-wasting disorder caused by mutations in the dystrophin gene. Studies have shown that bone marrow cells transplanted into lethally irradiated mdx mice, mouse model of DMD, can become part skeletal muscle myofibers. Whether human also this ability unknown. Here we report analysis biopsies from DMD patient (DMD-BMT1) who received transplantation at age 1 year for X-linked combined immune deficiency and was diagnosed with 12 years....
Leukemic cells in 15 to 25 percent of patients with acute lymphoblastic leukemia (ALL) express myeloid antigens as well lymphoid (the latter reflecting B-cell or T-cell lineage). The relations myeloid-antigen expression other features ALL and prognosis have been controversial.We analyzed clinical laboratory present at diagnosis 236 consecutive cases children. Immunophenotyping, including single- dual-fluorescence analyses, was used classify leukemic B T lymphoblasts also identify...