A5085000703- Mesenchymal stem cell research
- Osteoarthritis Treatment and Mechanisms
- Bone fractures and treatments
- Periodontal Regeneration and Treatments
- Bone and Joint Diseases
- Tissue Engineering and Regenerative Medicine
- Bone Tissue Engineering Materials
- Cancer Cells and Metastasis
- Orthopaedic implants and arthroplasty
- Hematopoietic Stem Cell Transplantation
- Spondyloarthritis Studies and Treatments
- Rheumatoid Arthritis Research and Therapies
- Electrospun Nanofibers in Biomedical Applications
- Autoimmune and Inflammatory Disorders Research
- Chronic Lymphocytic Leukemia Research
- Extracellular vesicles in disease
- Bone health and treatments
- Hematological disorders and diagnostics
- Bone Metabolism and Diseases
- Total Knee Arthroplasty Outcomes
- Knee injuries and reconstruction techniques
- Telomeres, Telomerase, and Senescence
- Neonatal Respiratory Health Research
- Psoriasis: Treatment and Pathogenesis
- Inflammatory mediators and NSAID effects
University of Leeds
2015-2024
NIHR Leeds Musculoskeletal Biomedical Research Unit
2015-2024
Bayhealth Foundation
2024
St James's University Hospital
2011-2022
Leeds Teaching Hospitals NHS Trust
2007-2022
Chapel Allerton Hospital
2006-2021
National Institute for Health Research
2021
Leeds General Infirmary
1995-2018
GTx (United States)
2017
Orthopaedic Trauma Association
2016
Abstract Objective There is an increased interest in rheumatology mesenchymal progenitor/stem cells (MPCs) and their roles rheumatic diseases, but little known about the phenotype of these vivo. The aim this study was to isolate characterize human bone marrow (BM) MPCs. Methods Fluorescence microscopy used identify putative MPCs among adherent BM cells. To purify them, a positive selection with antifibroblast microbeads used, combined fluorescence‐activated cell sorting (FACS) for...
Abstract Objective To investigate whether periosteal cells from adult humans have features of multipotent mesenchymal stem (MSCs) at the single‐cell level. Methods Cell populations were enzymatically released periosteum proximal tibia obtained human donors and then expanded in monolayer. Single‐cell–derived clonal by limiting dilution. Culture‐expanded cell tested for their growth potential expression conventional markers MSCs subjected to vitro assays multilineage potential. assess...
Abstract Objective To evaluate synovial fluid (SF) for the presence of mesenchymal progenitor cells (MPCs), to compare SF MPCs with bone marrow (BM) MPCs, and enumerate these in both inflammatory arthritis osteoarthritis (OA). Methods from 100 patients (53 rheumatoid [RA], 20 OA, 27 other arthropathies) was evaluated. establish multipotentiality, polyclonal single cell–derived cultures fibroblasts were examined by standard quantitative differentiation assays. Their phenotype before after...
Given the observed efficacy of culture-expanded multipotential stromal cells, also termed mesenchymal stem cells (MSCs), in treatment graft-versus host and cardiac disease, it remains surprising that purity potency characterization manufactured cell batches rather basic. In this paper, we will initially discuss surface molecular markers were proposed to serve as indicators MSC potency, terms their proliferative potential or ability differentiate into desired lineages. The second part paper...
Abstract Objective Arthritic synovial fluid (SF) contains mesenchymal stem cells (MSCs), which could simply reflect their shedding from diseased joint structures. This study used the bovine model to explore SF MSCs in health and enumerated them at earliest stages of human osteoarthritis (OA) radiographically normal joints. Methods Clonogenicity multipotentiality were compared with donor‐matched bone marrow (BM) single‐cell level. The colony‐forming unit–fibroblastic assay was for MSC...
Abstract Background: To study the biology of rare bone marrow (BM) multipotent mesenchymal stromal cells (MSCs), recognized protocols are needed. Colony‐forming unit‐fibroblast (CFU‐F) assays have historically been used for enumeration MSCs. However, need to isolate and further analyze MSCs requires new strategies based on cell surface markers. The purpose this work was verify phenotype BM in vivo develop flow cytometry‐based methods their evaluation. Methods: Pre‐enrichment with...
To test the hypothesis that CD45(low)CD271+ bone marrow multipotential stromal cells (MSCs) are abundant in trabecular niche and to explore their functional "fitness" health osteoarthritis (OA).Following enzymatic extraction, MSC release was evaluated using colony-forming unit-fibroblast (CFU-F) unit-osteoblast assays, flow cytometry, confocal microscopy. isolated by fluorescence-activated cell sorting were enumerated expanded under standard clonal conditions. Their proliferative osteogenic...
Objective Group 3 innate lymphoid cells (ILC3s) play a pivotal role in barrier tissues such as the gut and skin, two important sites of disease spondyloarthritis (SpA). This study was undertaken to investigate whether normal or injured human enthesis, key target tissue early SpA, harbors ILC3s entheseal soft adjacent perientheseal bone. Methods Interspinous ligament spinous process bone from donors with no systemic inflammatory were collected, enzymatically digested, immunophenotyped. The...
Background and Objectives: Stem cell technology offers a new hope for many chronic disorders patients.The types of stem cells are different with differences existing between each type.Mesenchymal (MSCs) represent one type adult that can be easily isolated, then re-transplanted to the patients.This potential their future application in treating without fear rejection possibility.MSCs isolated from sources e.g.bone marrow (BMSCs) adipose tissue (ADSCs).In present study we compared BMSCs ADSCs...
Abstract Objective Controversy surrounds the identity and functionality of rare bone marrow–derived multipotential stromal cells (BM‐MSCs), including their differentiation capabilities, relationship to pericytes hematopoiesis‐supporting cells, relevance culture‐expanded progeny in studies skeletal biology development cell‐based therapies. The aim this study was clarify nature candidate BM‐MSCs by profiling transcripts that reflect different aspects putative functions vivo. Methods Rare,...
The SpAs are genetically and therapeutically linked to IL-23, which in turn regulates IL-22, a cytokine that has been implicated the regulation of new bone formation experimental models. We hypothesize master regulator stem cells other niches, might also regulate human mesenchymal cell (MSC) osteogenesis.The effects IL-22 on vitro MSC proliferation, migration osteogenic differentiation were evaluated presence or absence IFN-γ TNF (to ascertain activity pro-inflammatory environments)....
In patients with osteoarthritis (OA), bone marrow lesions (BMLs) are intimately linked to disease progression. We hypothesized that aberrant multipotential stromal cell (also known as mesenchymal stem [MSC]) responses within tissue contributes BML pathophysiology. The aim of this study was investigate and non-BML native subchondral MSCs for numeric, topographic, in vitro functional, gene expression differences.Ex vivo 3T magnetic resonance imaging (MRI) the femoral heads 20 hip OA performed....
The utility of autologous chondrocytes for cartilage repair strategies in older subjects with osteoarthritis (OA) may be limited by both age-related and disease-associated decline chondrogenesis. aim this work was to assess OA Hoffa's fat pad as an alternative source chondroprogenitor cells compare it derived from different areas cartilage.Cartilage tissue digests were obtained 26 knee compared normal bone marrow (BM) mesenchymal stem (MSCs) respect their vitro colony-forming potential,...