A5035713735- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Rheumatoid Arthritis Research and Therapies
- Bone fractures and treatments
- Bone and Joint Diseases
- Cancer Cells and Metastasis
- Hematopoietic Stem Cell Transplantation
- Osteoarthritis Treatment and Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Bone Metabolism and Diseases
- Immune Cell Function and Interaction
- Tendon Structure and Treatment
- Systemic Lupus Erythematosus Research
- Monoclonal and Polyclonal Antibodies Research
- Wound Healing and Treatments
- T-cell and B-cell Immunology
- Tissue Engineering and Regenerative Medicine
- interferon and immune responses
- Hematological disorders and diagnostics
- COVID-19 Clinical Research Studies
- Immunotherapy and Immune Responses
- Cytokine Signaling Pathways and Interactions
- Autoimmune and Inflammatory Disorders Research
- Acute Myeloid Leukemia Research
- Spondyloarthritis Studies and Treatments
Mansoura University
2013-2024
Nottingham Trent University
2020-2024
University of Leeds
2013-2022
NIHR Leeds Musculoskeletal Biomedical Research Unit
2013-2022
Marymount University
2020
St James's University Hospital
2011-2019
Leeds Teaching Hospitals NHS Trust
2014-2018
Chapel Allerton Hospital
2015-2017
Laboratoire d'Informatique, de Robotique et de Microélectronique de Montpellier
2017
Mansoura University Hospital
2016
Immune evasion is now recognized as a key feature of cancer progression. In animal models, the activity cytotoxic lymphocytes suppressed in tumour microenvironment by immunosuppressive cytokine, Transforming Growth Factor (TGF)-β. Release from TGF-β-mediated inhibition restores anti-tumour immunity, suggesting therapeutic strategy for human cancer. We demonstrate that natural killer (NK) cells are inhibited TGF-β dependent manner following chronic contact-dependent interactions with vitro....
The SpAs are genetically and therapeutically linked to IL-23, which in turn regulates IL-22, a cytokine that has been implicated the regulation of new bone formation experimental models. We hypothesize master regulator stem cells other niches, might also regulate human mesenchymal cell (MSC) osteogenesis.The effects IL-22 on vitro MSC proliferation, migration osteogenic differentiation were evaluated presence or absence IFN-γ TNF (to ascertain activity pro-inflammatory environments)....
Uncultured mesenchymal stromal cells (MSCs) are increasingly used in therapies; however, the effects of donor age on their biological characteristics and gene expression remain unclear. The aim this study was to investigate age-related changes bone marrow (BM) MSCs following minimal or no culture manipulation. Iliac crest BM aspirated from 67 healthy donors (19-89 years old) directly for colony-forming unit-fibroblast (CFU-F) assay CD45lowCD271+ cell enumeration. colonies were analysed...
Variability in SARS-CoV-2 susceptibility and COVID-19 disease severity between individuals is partly due to genetic factors. Here, we identify 4 genomic loci with suggestive associations for 19 severity. Four of these 23 likely have an ethnicity-specific component. Genome-wide association study (GWAS) signals 11 colocalize expression quantitative trait (eQTLs) associated the 20 genes 62 tissues/cell types (range: 1:43 tissues/gene), including lung, brain, heart, muscle, skin as well...
<h3>Objectives</h3> The therapeutic goal for patients with rheumatoid arthritis (RA) is clinical remission. This best achieved by early diagnosis and appropriate intervention. RA associated dysregulation of T-cell subsets (naïve, regulatory (Treg) inflammation-related cells (IRC)) in the disease. Our aim was to test hypothesis that subset quantification can predict achievement remission treatment RA. <h3>Methods</h3> were quantified 108 drug-naïve, commencing methotrexate (MTX) or...
Large bone defects are ideally treated with autografts, which have many limitations. Therefore, osteoconductive scaffolds loaded autologous marrow (BM) aspirate increasingly used as alternatives. The purpose of this study was to compare the growth multipotential stromal cells (MSCs) from unprocessed BM on a collagen-containing bovine scaffold (Orthoss(®) Collagen) non-collagen-containing scaffold, Orthoss(®) . Another synthetic Vitoss(®) included in comparison. Colonization by MSCs (n = 23...
Bone marrow-Multipotential stromal cells (BM-MSCs) are increasingly used to treat complicated fracture healing e.g., non-union. Though, the quality of these autologous is not well characterized. We aimed evaluate bone healing-related capacities non-union BM-MSCs. Iliac crest-BM was aspirated from long-bone patients with normal (U) or non-united (NU). Uncultured (native) CD271highCD45low passage-zero cultured BM-MSCs were analyzed for gene expression levels, and functional assays conducted...
Abstract Rheumatoid arthritis (RA) is a chronic inflammatory disorder with poorly defined aetiology characterised by synovial inflammation variable disease severity and drug responsiveness. To investigate the peripheral blood immune cell landscape of early, naive RA, we performed comprehensive clinical molecular profiling 267 RA patients 52 healthy vaccine recipients for up to 18 months establish high quality sample biobank including plasma, serum, cells, urine, genomic DNA, RNA from whole...
The potential use of bone progenitors, multipotential stromal cells (MSCs) helping spine fusion is increasing, but convenient MSC sources and effective processing methods are critical factors yet to be optimised. aim this study was test the effect marrow on abundance compare differentiation capabilities vertebral body-bone (VB-BM) MSCs versus iliac crest-bone (IC-BM) MSCs. We assessed red blood cell lysis (ammonium chloride, AC) density-gradient centrifugation (Lymphoprep™, LMP), extracted...
Bone marrow aspirates and concentrates are increasingly being used for musculoskeletal regenerative therapies, providing bone cartilage progenitors. However, the quality of these samples remains imprecise within clinical settings. As there is a need development method counting CD45CD271 cells was optimized tested as an indicator sample quality.Bone were collected from 54 donors (28 male 26 female; median age 48 years). The reagent concentrations fast staining, acoustic-focusing flow...
Interleukin-7 (IL-7) is a cytokine essential for T-cell lymphopoiesis, survival and polarization with an emerging role in autoimmunity. We previously demonstrated reduced levels of circulating IL-7 rheumatoid arthritis (RA), although high amounts are expressed joints, suggesting differences between systemic synovial effects. observed healthy 48% RA patients clinical remission (CR) aimed to investigate the consequences deficiency on responses. used active disease CR presenting various IL-7,...
ABSTRACT This study investigates how mesenchymal stem cell's (MSCs) proliferation and migration abilities are influenced by various platelet products (PP). Donor‐matched, clinical‐, control laboratory‐standard PPs were generated assessed based on their leukocyte concentrations. Bone marrow derived MSCs exposed to these PP quantify effect in vitro MSC migration. An adapted colony forming unit fibroblast (CFU‐F) assay was carried out bone aspirate using clinical‐standard PP‐loaded electrospun...
Barrier membranes are popularly used for guided bone regeneration (GBR).However, more knowledge is needed to assess how these could be of therapeutic value when populated with native multipotent stromal cells (MSCs), particularly in the orthopaedic field.The present manuscript investigated activities human marrow-multipotent (BM-MSCs) loaded on two differently structured pure collagen membranes.A crosslinked membrane (CS) was tested versus a non-crosslinked bilayer membrane, Bio-Gide ®...
Abstract Osteoarthritis (OA) is the most common degenerative joint disorder. Multipotential stromal cells (MSCs) have a crucial role in repair, but how OA severity affects their characteristics remains unknown. Knee provides good model to study this, as osteochondral damage commonly more severe medial weight-bearing compartment compared lateral side of joint. This utilised vitro functional assays, cell sorting, gene expression and immunohistochemistry compare MSCs from femoral condyles....
Multipotential stromal cells (MSCs) demonstrate strong immunomodulation capabilities following culture expansion. We have previously demonstrated that human cancellous bone fragments (CBFs) clinically used as viable allografts for spinal fusion resident MSCs exhibit T cell after monolayer This study investigated the immunomodulatory ability of these CBFs without MSC culture-expansion. CD4 positive were induced to proliferate using CD3/CD28 stimulation and added at different ratios per gram...