Structured hydrogels showing form stability and elastic properties individually tailorable on different length scales are accessible in a one-step process. They support cell adhesion differentiation display growing pore size during degradation. In vivo experiments demonstrate their efficacy biomaterial-induced bone regeneration, not requiring addition of cells or growth factors.

Gelatin is a non-immunogenic and degradable biopolymer, which widely applied in the biomedical field e.g. for drug capsules or as absorbable hemostats. However, gelatin materials present limited hardly reproducible mechanical properties especially aqueous systems, particularly caused by uncontrollable partial renaturation of collagen-like triple helices. Therefore, mechanically demanding applications gelatin-based materials, such vascular patches, i.e. hydrogel films that seal large...

The design of photo-active collagen systems is presented as a basis for establishing biomimetic materials with varied network architecture and programmable macroscopic properties. Following in-house isolation type I collagen, reaction vinyl-bearing compounds backbone rigidity, i.e. 4-vinylbenzyl chloride (4VBC) glycidyl methacrylate (GMA), was carried out. TNBS colorimetric assay, 1H-NMR ATR-FTIR confirmed covalent tunable functionalization lysines. Depending on the extent functionalization,...

Thiol-ene photo-click hydrogels were prepared via step-growth polymerisation using thiol-functionalised type-I collagen and 8-arm poly(ethylene glycol) norbornene-terminated (PEG-NB), as a potential injectable regenerative device. Type-I was by ring opening reaction with 2-iminothiolane (2IT), whereby up to 80 Abs.% functionalisation 90 RPN% triple helical preservation recorded 2,4,6-Trinitrobenzenesulfonic acid (TNBS) colorimetric assay circular dichroism (CD). Type, i.e., either...

Currently two factors hinder the use of collagen as building block regenerative devices: potential antigenicity, and limited mechanical strength in aqueous environment. Polymeric is naturally found cross-linked state mechanically tougher than monomeric, cross-link-free (acid-soluble) ex vivo. The antigenicity collagen, on other hand, mainly ascribed to inter-species variations amino acid sequences, which are primarily located non-helical terminal telopeptides. Although these can be removed...

Continuous wound monitoring is one strategy to minimise infection severity and inform prompt variations in therapeutic care following diagnosis. However, integration of this functionality dressings still challenging. We hypothesised that a theranostic dressing could be realised by integrating collagen-based contact layer with previously demonstrated healing capability, halochromic dye, i.e. bromothymol blue (BTB), undergoing colour change infection-associated pH changes (pH: 5–6 ➔ >7). Two...

Chemical crosslinking of collagen is a general strategy to reproduce macroscale tissue properties in physiological environment. However, simultaneous control protein conformation, material and biofunctionality highly challenging with current synthetic strategies. Consequently, the potentially-diverse clinical applications collagen-based biomaterials cannot be fully realised. In order establish defined biomacromolecular systems for mineralised applications, type I was functionalised...

Biological hydrogels have been increasingly sought after as wound dressings or scaffolds for regenerative medicine, owing to their inherent biofunctionality in biological environments. Especially moist healing, the ideal material should absorb large amounts of exudate while remaining mechanically competent situ . Despite hydration, however, current still leave much be desired terms mechanical properties physiological conditions. To address this challenge, a multi-scale approach is presented...

One of the limitations electrospun collagen as bone-like fibrous structure is potential triple helix denaturation in fibre state and corresponding inadequate wet stability even after crosslinking. Here, we have demonstrated feasibility accomplishing wet-stable fibres by spinning diacid-based crosslinking helices, whereby ability to act bone-mimicking mineralisation system has also been explored. Circular dichroism (CD) nearly complete retention resulting wet-spun fibres, chemically...

Manuka honey (MH) is used as an antibacterial agent in bioactive wound dressings via direct impregnation onto a suitable substrate. MH provides unique activity when compared with conventional honeys, owing partly to one of its constituents, methylglyoxal (MGO). Aiming investigate antibiotic-free antimicrobial strategy, we studied the both and MGO (at equivalent concentrations) applied physical coating nonwoven fabric dressing. When physically coated on cellulosic hydroentangled fabric, it...

The formation of naturally derived materials with wet stable fibrous architectures is paramount in order to mimic the features tissues at molecular and microscopic scale. Here, we investigated wet-spun fibres based on collagen-derived polypeptides comparable chemical composition varied weight. Gelatin hydrolysed fish collagen (HFC) were selected as widely available linear amino-acidic chains high low weight, respectively, functionalised fibre state preserve material geometry physiological...

The design of exudate-managing wound dressings is an established route to accelerated healing, although such remains a challenge from material and manufacturing standpoints. Aiming towards the clinical translation knowledge gained in vitro with highly-swollen rat tail collagen hydrogels, this study investigated healing capability diabetic mouse model telopeptide-free, protease-inhibiting networks. 4-Vinylbenzylation UV irradiation type I atelocollagen (AC) led hydrogel networks chemical...

Antimicrobial photodynamic therapy (APDT) is a promising antibiotic-free strategy for broad-spectrum infection control in chronic wounds, minimising bacterial resistance risks. However, rapid photosensitiser diffusion, tissue staining, side toxicity, and short-lived antimicrobial effects present significant clinical limitations integrating APDT into wound dressings. To address these challenges, we the design of bespoke polyvinyl alcohol (PVA) derivative conjugated with both phenothiazine...

Abstract Polyester urethane networks are versatile polymer systems as it is possible to tailor their mechanical properties and hydrolytic degradation profile. For biomedical applications, the biodegradability well thermomechanical of during course importance. Therefore, we investigated change based on star‐shaped precursors rac ‐dilactide diglycolide, ε ‐caprolactone, or p ‐dioxanone, respectively, degradation. Degradation rate were tailored by crosslink density, comonomers, changing glass...

Biopolymer-based systems with adjustable macroscopic properties that can be varied in a wide range using only small changes chemical composition are promising candidates for biomaterial-induced autoregeneration. Glycidylmethacrylated gelatin is photopolymerized the addition of PEG mono- or dimethacrylate to form co-networks pH = 7.4 PBS. The degree swelling (Q) and water uptake (H) PBS at 37 °C tailorable PEGDMA (Q ≈ 250-650 vol%), while storage modulus swollen networks 3 adjusted by...

The selective covalent coupling of hydroxamic acid functions on to methacrylated type I collagen led UV-cured networks with inherent MMP-modulating capability and enhanced proteolytic stability.

Poly-dl-lactic acid (PDLLA) was blended with type I collagen to attempt overcome the instantaneous gelation of electrospun scaffolds in biological environments. Scaffolds based on blends and PDLLA were investigated for material stability cell culture conditions (37 °C; 5% CO2) which post-electrospinning glutaraldehyde crosslinking also applied. The resulting wet-stable webs cultured bone marrow stromal cells (HBMSC) five weeks. Scanning electron microscopy (SEM), confocal laser scanning...

Polymer-based hydrogels have been widely applied for chronic wound therapeutics, due to their well-acclaimed exudate management capability. At the same time, there is still an unmet clinical need simple diagnostic tools assist decision-making at point of care and deliver on vision patient-personalised management. To explore this challenge, we present a one-step synthetic strategy realise redox-responsive, hyaluronic acid (HA)-based hydrogel that sensitive environment-related variations in...

Electrospun nanofibrous membranes of natural polymers, such as gelatin, are fundamental in the design regenerative devices. Crosslinking electrospun fibres from gelatin is required to prevent dissolution water, retain original nanofibre morphology after immersion and improve thermal mechanical properties, although this still challenging accomplish a controlled fashion. In study, we have investigated scalable manufacture structural stability aqueous environment UV-cured membrane fabricated by...

Antimicrobial biomaterials are critical to aid in the regeneration of oral soft tissue and prevent or treat localized bacterial infections. With rising trend antibiotic resistance, there is a pressing clinical need for new antimicrobial chemistries biomaterial design approaches enabling on-demand activation antibiotic-free functionality following an infection that environment-friendly, flexible commercially viable. This study explores feasibility integrating bioresorbable electrospun polymer...

As problems of antibiotic resistance increase, a continuing need for effective bioactive wound dressings is anticipated the treatment infected chronic wounds. Naturally derived antibacterial agents, such as Manuka honey, consist mixture compounds, more than one which can influence antimicrobial potency. The non-peroxide bacteriostatic properties honey have been previously linked to presence methylglyoxal. incorporation methylglyoxal functional additive during fibre production was explored...