A5102997543- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Oral microbiology and periodontitis research
- Antimicrobial Resistance in Staphylococcus
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Antibiotic Use and Resistance
- Escherichia coli research studies
- Genomics and Phylogenetic Studies
- Pneumonia and Respiratory Infections
- Dental Health and Care Utilization
- Urinary Tract Infections Management
- Tuberculosis Research and Epidemiology
- Gut microbiota and health
- Infections and bacterial resistance
- Tryptophan and brain disorders
- Vibrio bacteria research studies
- Metabolomics and Mass Spectrometry Studies
- Salmonella and Campylobacter epidemiology
- Neonatal and Maternal Infections
- Salivary Gland Disorders and Functions
Chinese People's Liberation Army
2014-2025
Chinese PLA General Hospital
2015-2025
Guangzhou University of Chinese Medicine
2024
Guangzhou University
2024
Peking University Third Hospital
2020
Peking University
2020
Beijing University of Chinese Medicine
2018
People's Liberation Army No. 150 Hospital
2014
Army Medical University
2013
117th Hospital of People's Liberation Army
2011
In order to obtain a better molecular understanding of recurrent urinary tract infection (RUTI), we collected 75 cases with repeatedly occurring uncomplicated UTI. The genetic relationships among uropathogenic Escherichia coli (UPEC) isolates were analyzed by pulsed-field gel electrophoresis. While 39 (52%) the RUTI defined as "persistence" same strain primary infecting strain, 36 (48%) characterized "reinfection" new that is different from strain. We then examined antimicrobial...
In China, the spread and outbreak of OXA-48-producing Enterobacteriaceae remains largely unknown.OXA-48-producing isolates were analyzed for genetic relatedness by pulsed-field gel electrophoresis (PFGE), antimicrobial susceptibility E-test, sequence type (ST) multilocus typing. S1-PFGE southern blotting used plasmid profiling, PCR subsequent sequencing performed to determine environment blaOXA-48 gene.In total, 37 non-duplicated K. pneumoniae (OXAKp) recovered. From December 2013 August...
The distribution of methicillin-resistant Staphylococcus aureus (MRSA) clones is dynamic and geographically unique. To understand the changing epidemiology MRSA infections in China, we performed a prospective, multicity surveillance study with molecular typing phenotypic analysis to determine association major prevalent their antimicrobial resistance profiles. A total 517 S. isolates collected between January 2009 March 2012 from six cities China were subjected antibiogram typing, including...
ABSTRACT Accurate species-level identification of Enterobacter cloacae complex (ECC) is crucial for related research. The classification ECC based on strain-to-strain phylogenetic congruence, as well genomic features including average nucleotide identity (ANI) and digitalized DNA-DNA hybridization (dDDH). ANI dDDH derived from whole-genome sequencing have emerged a reliable metric assessing genetic relatedness between genomes are increasingly recognized standard species delimitation. Up to...
ABSTRACT Acinetobacter baumannii , a prominent nosocomial pathogen renowned for its extensive resistance to antimicrobial agents, poses significant challenge in the accurate prediction of (AMR) from genomic data. Despite thorough researches on molecular mechanisms AMR, gaps remain our understanding key contributors. This study utilized rule-based and three machine learning models predict AMR phenotypes, aiming decipher factors associated with AMR. Genomes antibiotic phenotypes 1,012 public...
Introduction The impact of the hypermucoviscosity (HMV) phenotype in ST11-KL47 carbapenem-resistant Klebsiella pneumoniae (CRKp) pathogenicity warrants investigation for public health risk assessment. Methods We analyzed 230 clinical CRKp to identify key factor mucoviscosity acquisition via comparative genomic analysis. Sedimentation value served as objective index quantify HMV. virulence vivo was assessed using Galleria mellonella and mouse infection models. employed genome engineering,...
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has recently been introduced in diagnostic microbiology laboratories for the identification bacterial and yeast strains isolated from clinical samples. This study aimed to evaluate accuracy MALDI-TOF MS diagnosis by comparing it with commonly-used VITEK 2 or gene sequencing.The performances were compared retrospectively identifying routine isolates. Discrepancies analyzed sequencing analysis 16S...
Staphylococcus aureus belongs to one of the most common bacteria causing healthcare and community associated infections in China, but their molecular characterization has not been well studied. From May 2011 June 2012, a total 322 non-duplicate S. isolates were consecutively collected from seven tertiary care hospitals cities with distinct geographical locations including 171 methicillin sensitive (MSSA) 151 MRSA isolates. All characterized by spa typing. The presence virulence genes was...
Abstract Background The spread and outbreak of Enterobacteriaceae producing OXA-48-like carbapenemases have become more prevalent in China. Results A total 62 non-duplicated OXA-232-producing K. pneumoniae (OXA232Kp) were isolated between 2015 2017. An OXA232Kp was observed burn ICU. isolates all belongs to ST15 categorized into two PFGE types (A B). Type dominated the isolates, which contained 61 clinical divided 10 subtypes (A1-A10). In addition, most strains exhibited low-level...
Abstract Background The molecular characteristics of carbapenem-resistant Escherichia coli (CREco) remain unclear. Methods We conducted a multi-center bacterial resistance monitoring project from 2015 to 2017.The minimum inhibitory concentrations ofCREco were determined bybroth microdilution method. genome sequencing CREcoisolates was performed, and single-nucleotide polymorphism (SNP) analyzed. Results A total 144CREcoisolatescollected 10 cities in China involved this study. ST167 ( n = 43)...
New Delhi metallo-β-lactamase (NDM)-producing Enterobacteriaceae (NPE) shows prevalence in China. Little is known about the mechanisms related to spread of NPE. Recently, a total 51 non-duplicated NPE isolates were collected from tertiary-care hospital China and analysed for genetic relatedness by PFGE, antimicrobial susceptibility Etest sequence type multilocus typing. S1-PFGE Southern blot analysis or PCR amplification used plasmid profiling. Between 2014 2015, 22 Escherichia coli, 10...
To evaluate the efficacy of ceftazidime-avibactam (CZA) and aztreonam-avibactam (AZA) against bloodstream infections (BSIs) or lower respiratory tract (LRTIs) - caused by extensive drug-resistant pan (XDR/PDR) Pseudomonas aeruginosa.The two-fold dilution method was used to determine minimum inhibitory concentrations (MICs) CZA/AZA XDR/PDR P. aeruginosa. Whole-genome sequencing analyze resistance determinants each isolate. Monte Carlo simulations (MCSs) were probability target attainment...
Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) MS systems was not officially launched for diagnostic use in clinical microbiology laboratories China until 2012. Here, we report the findings from first large-scale evaluation study VITEK routine bacterial identification two major centres Beijing and Hong Kong. A total 2266 unique isolates representing 56 genera 127 species were analysed, results compared to those obtained by 2. Any discrepancies resolved 16S rRNA...
This study was designed to demonstrate the characteristics of qacA/B-positive Staphylococcus aureus in China. One hundred and forty-five MRSA 178 MSSA from clinical specimens seven hospitals different regions China, 70 superficial sites patients 106 environmental samples an ICU were collected screened for presence qacA/B gene. The isolates 72 randomly selected qacA/B-negative control further characterized by MLST, spa typing detection toxin genes, as well antimicrobial chlorhexidine...
Background Carbapenem-resistant Klebsiella pneumoniae (CRKP) is an important pathogen causing hospital-associated outbreaks worldwide. The spread of K. carbapenemase-2 (KPC-2)-producing CRKP primarily associated with sequence type (ST) 11. Methods A total 152 KPC-2-producing ST11 isolates were collected from the respiratory department a tertiary care hospital in Beijing, China between 2009 and 2018. genome sequencing these was performed on HiSeq X Ten sequencer. Multilocus typing (MLST),...
Twelve nonduplicated KPC-2-producing enterobacterial isolates, including three Escherichia coli, two Citrobacter freundii, Enterobacter cloacae, four aerogenes, and one Klebsiella oxytoca, were collected from various clinical samples within 18 months (March 2011 to September 2012). Two of the 12 patients died infections caused by pathogens, while rest with pathogens improved or cured. The majority isolates exhibited a high-level resistance oxyimino-cephalosporins carbapenems, possessed...
Vancomycin-resistant Enterococcus (VRE) has been identified in China. However, little is known about the spread of VRE isolates.The genetic relatedness vancomycin-resistant faecium (VREfm) isolates was analyzed by pulsed-field gel electrophoresis (PFGE), their antimicrobial susceptibilities were E-test and VITEK 2 AST-GP67 test Kit, sequence types (STs) investigated multilocus typing (MLST). S1-PFGE used for plasmid profiling, PCR subsequent sequencing performed to identify virulence genes.A...
BACKGROUND:The aim of this study was to examine the behavior circulating inflammatory mediators and exclude gram-positive from gram-negative bloodstream infections. Results may be helpful in selection optimal specific antibiotic therapies. MATERIAL AND METHODS:Mice (25–27 g) were randomized 3 groups infected with Staphylococcus aureus (S. aureus) ATCC 25923, Escherichia coli (E. coli) 25922, or phosphate-buffered saline (PBS). The white blood cell count (WBC) concentrations serum C-reactive...