A5100341179- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Microbial Metabolism and Applications
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Biochemical and Structural Characterization
- Bacteriophages and microbial interactions
- Bacterial Identification and Susceptibility Testing
- Fungal Infections and Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Pharmaceutical and Antibiotic Environmental Impacts
- Probiotics and Fermented Foods
- Milk Quality and Mastitis in Dairy Cows
- Immune Response and Inflammation
- Antifungal resistance and susceptibility
- Berberine and alkaloids research
- Bacterial Genetics and Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Gut microbiota and health
- Enterobacteriaceae and Cronobacter Research
- Infective Endocarditis Diagnosis and Management
- Pneumonia and Respiratory Infections
Peking University
2013-2023
Peking University People's Hospital
2013-2023
Jilin Agricultural University
2023
Shenyang Agricultural University
2020
Marked increases in fluconazole resistance Candida tropicalis have been recently reported. In this study, the molecular mechanisms behind were investigated. Twenty-two C. clinical isolates, including 12 fluconazole-resistant isolates and 10 fluconazole-susceptible collected from a tertiary care teaching hospital Beijing between 2013 2017. Antifungal susceptibility testing, multilocus sequence typing, ERG11 amplification sequencing, quantitative real-time reverse transcription-polymerase...
ABSTRACT Acinetobacter baumannii , a prominent nosocomial pathogen renowned for its extensive resistance to antimicrobial agents, poses significant challenge in the accurate prediction of (AMR) from genomic data. Despite thorough researches on molecular mechanisms AMR, gaps remain our understanding key contributors. This study utilized rule-based and three machine learning models predict AMR phenotypes, aiming decipher factors associated with AMR. Genomes antibiotic phenotypes 1,012 public...
Small-colony variants (SCVs) of Staphylococcus aureus have drawn increasing research attention. Owing to their slow growth, atypical colony morphology, and unusual metabolic characteristics, SCVs often cause confusion in the laboratory.
Macrophages are professional phagocytic cells that play a critical role in initiating immune responses by presenting antigen and clearance. The macrophages can be targeted for immunomodulation beneficial microbes, such as probiotics. aim of this study is to investigate the protective effect Saccharomyces boulardii against Clostridium perfringens infection avian macrophage cell line HD11. In study, HD11 were prestimulated with S. 6 h then infected C. 3 h. Results showed enhanced phagocytosis...
Linezolid-resistant enterococci pose great challenges in clinical practice. The aim of this study is to the mechanisms underlying resistance and genetic environment antimicrobial gene linezolid-resistant enterococci.
Daptomycin resistant Staphylococcus aureus strains are well documented, but have not been reported in China. To elucidate the evolution adaptability and fitness cost of daptomycin S. aureus, three susceptible strains, Pre3 (MRSA, ST239-t037), Pre5 Pre14b (MSSA, ST188-t189), were isolated from patients with bloodstream infections, serially passaged MH broth a gradient concentration to select for resistance. Highly mutants obtained after screening 34 passages. The MICs increased 0.5 μg/ml...
Staphylococcus aureus is a major etiological agent of bovine mastitis worldwide. In this study, 37 strains S. Aureus resistant to penicillin were isolated from cases in Gansu Province for investigating blaZ and virulence-related genes, including tst, eta, etb, lukPV, lukED, lukM, hla, hlb, hld, edin. Antibiotic resistance was based on disk diffusion method virulence-associated genes studied by polymerase chain reaction. Penicillin gene detected 35/37 (94.6%) penicillin-resistant isolates....
Considerable efforts have been exerted to identify factors contributing the success of epidemic Staphylococcus aureus clones, however, comparative phenotypic studies lack representation owing small number strains. Large-scale strain collections focused on description genomic characteristics.
A comparative genomic microarray comprising 2,457 genes from two whole genomes of S. aureus was employed for the genome hybridization analysis 50 strains divergent clonal lineages, including methicillin-resistant (MRSA), methicillin-susceptible (MSSA), and swine in China. Large-scale validation confirmed via polymerase chain reaction 160 representative clinical strains. All were clustered into seven different complexes by phylogenetic tree analysis. Thirteen gene clusters specific to clones....
The aim of this study was to identify the subtype, characterize antimicrobial resistance, determine virulence gene distribution, and analyze biofilm production Staphylococcus aureus isolates from bovine mastitis milk samples in Liaoning Province China.In total, 56 Staph. were collected identified study; divided into different spa types based on sequence polymorphic X region gene. Additionally, susceptibility investigated using broth microdilution method, 18 genes detected PCR. Biofilm...
Objective To investigate the curative effect and security of Linezolid on severe pneumonia in elderly. Methods Three cases male advanced aged (all are hospital acquired pneumonia) from Zhongnan Hospital Wuhan university during January 2015 to 2016 were selected. They first treated with third generation cephalosporins/β-lactamase inhibitor combined carbapenems broad-spectrum antibiotics teicoplanin intravenous infusion for initial empirical treatment, sputum bacterial culture obtained....
Background: Staphylococcus aureus has great population diversity, but relative few clones dominate in specific regions. A dramatic decrease S. ST239 and the emergence of ST59 hospitals been observed recent years.Methods: total 142 strains were selected from our seven-year national surveillance bloodstream infections (n=983). combination phenotypic genomic analyse was applied to identify factors that contribute success epidemic clones, ST398.Findings: Specific genetic traits such as...