A5100778737- Antibiotic Resistance in Bacteria
- Malaria Research and Control
- Mosquito-borne diseases and control
- Antibiotics Pharmacokinetics and Efficacy
- Pharmaceutical and Antibiotic Environmental Impacts
- vaccines and immunoinformatics approaches
- Bacterial Identification and Susceptibility Testing
- HIV Research and Treatment
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Plant Pathogenic Bacteria Studies
- Tuberculosis Research and Epidemiology
- Complement system in diseases
- Infections and bacterial resistance
- Escherichia coli research studies
- Immunotherapy and Immune Responses
- Urban Green Space and Health
- Enterobacteriaceae and Cronobacter Research
- Chronic Kidney Disease and Diabetes
- Parasites and Host Interactions
- Cardiomyopathy and Myosin Studies
- Genomics and Phylogenetic Studies
- Diverse Aspects of Tourism Research
- Vector-borne infectious diseases
Guangdong Provincial People's Hospital
2024-2025
Peking University People's Hospital
2016-2025
Peking University
2016-2025
Guangdong Academy of Medical Sciences
2024-2025
Yunnan University
2025
Guangzhou University of Chinese Medicine
2023-2024
Shenzhen University
2022-2024
Southern Medical University
2024
Peking Union Medical College Hospital
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2024
Abstract Colistin represents one of the few available drugs for treating infections caused by carbapenem-resistant Enterobacteriaceae . As such, recent plasmid-mediated spread colistin resistance gene mcr-1 poses a significant public health threat, requiring global monitoring and surveillance. Here, we characterize distribution using data set 457 mcr-1- positive sequenced isolates. We find in various plasmid types but identify an immediate background common to all sequences. Our analyses...
Carbapenem-resistant Enterobacteriaceae (CRE) strains are a major threat to global health. The development of effective control measures requires more detailed phenotypic and genotypic characterization CRE. CRE isolates were collected from 65 hospitals in 25 provinces across China between January 1, 2012, December 31, 2016. characterized by antimicrobial susceptibility testing multilocus sequence typing. Genes encoding carbapenemases, mobilized colistin resistance (mcr-1), β-lactamases...
Hypervirulent Klebsiella pneumoniae (hvKP) is traditionally defined by hypermucoviscosity, but data based on genetic background are limited. Antimicrobial-resistant hvKP has been increasingly reported not yet systematically studied. K. isolates from bloodstream infections, hospital-acquired pneumonia, and intra-abdominal infections were collected 10 cities in China during February to July 2013. Clinical medical records. All investigated antimicrobial susceptibility testing, string test,...
Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) have been increasingly reported in China. Here, a multicentre, longitudinal surveillance study on CR-hvKP is described.We retrospectively investigated carbapenem-resistant K. (CRKP) 56 centres across China during 2015-17 and screened the virulence genes (iucA, iroN, rmpA rmpA2) for presence of plasmids. Hypermucoviscosity, serum killing Galleria mellonella lethality experiments were conducted to identify among strains with...
Abstract The mobile resistance gene bla NDM encodes the enzyme which hydrolyses carbapenems, a class of antibiotics used to treat some most severe bacterial infections. is globally distributed across variety Gram-negative bacteria on multiple plasmids, typically located within highly recombining and transposon-rich genomic regions, leads dynamics underlying global dissemination remain poorly resolved. Here, we compile dataset over 6000 genomes harbouring gene, including 104 newly generated...
The acquisition of exogenous mobile genetic material imposes an adaptive burden on bacteria, whereas the adaptational evolution virulence plasmids upon entry into carbapenem-resistant Klebsiella pneumoniae (CRKP) and its impact remains unclear. To better understand in CRKP, we characterize utilizing a large genomic data containing 1219 K. from our long-term surveillance publicly accessible databases. Phylogenetic evaluation unveils associations between distinct serotypes. sub-lineage...
Carbapenem-resistant Klebsiella pneumonia (CRKP) is a global threat that varies by region. The distribution, evolution, and clinical implications of the ST11 CRKP clone remain obscure. We conducted multicenter molecular epidemiological survey using isolates obtained from 28 provinces municipalities across China between 2011 2021. integrated sequences public databases performed genetic epidemiology analysis CRKP. Among CRKP, KL64 serotypes exhibited considerable expansion, increasing 1.54% to...
BackgroundEmergence of KPC-2 and NDM-1-coproducing carbapenem-resistant Klebsiella pneumoniae (KPC-2-NDM-1-CRKP) has escalated threat CRKP to healthcare. Currently, only 4 isolates had been reported sporadically. It remains unclear how KPC-2-NDM-1-CRKPs emerged, whether they are stable have transmission capacity.MethodsPCR Sanger sequencing was used screen carbapenemase genes for 2057 isolates, identify KPC-2-NDM-1-CRKPs. The clinical information the patients collected from medical records....
Metagenomic next-generation sequencing (mNGS) of plasma cell-free DNA has emerged as a promising diagnostic technology for bloodstream infections. However, major limitation current mNGS assays is the high rate false-positive results due to contamination.We made novel use 3 control groups-external negative controls under long-term surveillance, blood samples with result in conventional tests, and group healthy people-that were combined dedicated distinguishing contaminants arising from...
Tigecycline is considered one of the last-resort antimicrobials for carbapenem-resistant K. pneumoniae. Plasmid-mediated tigecycline resistance remains largely unclear. Here, by utilizing whole genome sequencing, we report a plasmid-mediated mechanism, 6,489 bp Resistance-nodulation-division family (RND) efflux pump (tmexCD1-toprJ1 pump), that confers transferable in K pneumoniae isolated from patients and chickens. In addition, identified high prevalence plasmids co-harbouring both...
Cefiderocol has been approved in the United States and Europe but not China. We aim to evaluate carbapenem-resistant Enterobacterales (CRE) susceptibility cefiderocol provide baseline data investigate resistance mechanism. From 2018 2019, 1,158 CRE isolates were collected from 23 provinces municipalities across The MICs of antimicrobials determined via agar dilution broth microdilution methods. Whole-genome sequencing was performed for 26 cefiderocol-resistant Escherichia coli Clone...
To detect pre-patent parasitemia, we developed a real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for the asexual 18S ribosomal RNA (rRNAs) of Plasmodium falciparum . Total nucleic acids extracted from whole blood were combined with control and tested by qRT-PCR. The assay quantified > 98.7% parasite-containing samples to ±0.5 log 10 parasites/mL nominal value without false positives. analytical sensitivity was ≥ 20 parasites/mL. coefficient variation 0.6%...
This study identified and characterized mcr-1-positive Enterobacteriaceae (MCRPE) carbapenemase-producing (CPE) in hospital sewage water. Influent effluent samples were collected from five tertiary hospitals Beijing December 2016. Samples screened for MCRPE CPE using antibiotic selection media. Results confirmed by PCR amplification of β-lactamase colistin resistance (mcr-1 mcr-2) genes sequencing. Antimicrobial susceptibility testing, MLST, conjugation plasmid typing...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is a highly malignant neoplasm characterized by poor prognosis and limited therapeutic strategy. The PDAC tumor microenvironment presents complex heterogeneity, where neutrophils emerge as the predominant constituents of innate immune cell population. Leveraging power single-cell RNA-seq, spatial multi-omics approaches, we included both published datasets our in-house patient cohorts, elucidating inherent heterogeneity in formation neutrophil...
Astragalus membranaceus (AM) shows potential therapeutic benefits for managing diabetic kidney disease (DKD), a leading cause of failure with no cure. However, its comprehensive effects on renal outcomes and plausible mechanisms remain unclear. This systematic review meta‐analysis aimed to synthesize the AM in DKD animal models. Seven electronic databases were searched studies until September 2023. Risk bias was assessed based SYRCLE's Bias tool. Standardized mean difference (SMD) or (MD)...
Vaccine-induced protection against diseases like malaria, AIDS, and cancer may require induction of Ag-specific CD8(+) CD4(+) T cell Ab responses in the same individual. In humans, a recombinant Plasmodium falciparum circumsporozoite protein (PfCSP) candidate vaccine, RTS,S/adjuvant system number 2A (AS02A), induces cells Abs, but no measurable by CTL or short-term (ex vivo) IFN-gamma ELISPOT assays, partial protection. P. DNA vaccines elicit these We report that sequential immunization with...
Although coexistence of blaNDM-5 and mcr-1 in Escherichia coli has been reported, little is known about the fitness virulence such strains. Three carbapenem-resistant (GZ1, GZ2 GZ3) successively isolated from one patient 2015 were investigated for microbiological virulence. GZ1 also resistant to colistin. To verify association between plasmids fitness, growth kinetics transconjugants performed. We analyzed genomic sequences GZ3 using PacBio sequencing. (ST10) co-harbored mcr-1, while...