A5091038325- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Coral and Marine Ecosystems Studies
- Wastewater Treatment and Nitrogen Removal
- Genomics and Phylogenetic Studies
- Marine Biology and Ecology Research
- Aquatic Ecosystems and Phytoplankton Dynamics
- Advanced biosensing and bioanalysis techniques
- Ocean Acidification Effects and Responses
- Environmental DNA in Biodiversity Studies
- Plant Disease Resistance and Genetics
- Marine Bivalve and Aquaculture Studies
- Algal biology and biofuel production
- Alkaline Phosphatase Research Studies
- Photosynthetic Processes and Mechanisms
- Aquaculture disease management and microbiota
- Marine Sponges and Natural Products
- Marine Toxins and Detection Methods
- Electrochemical Analysis and Applications
- Epigenetics and DNA Methylation
- Microbial Natural Products and Biosynthesis
- Diatoms and Algae Research
- Spectroscopy and Quantum Chemical Studies
- Bacterial Identification and Susceptibility Testing
Xiamen University
2015-2024
Fudan University
2012-2024
State Key Laboratory of Surface Physics
2021-2024
Southwest Medical University
2024
Laboratoire des Sciences du Climat et de l'Environnement
2024
Laboratoire de Mesure du Carbone 14
2024
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2024
CEA Paris-Saclay
2024
Fujian Medical University
2022-2023
National Health and Family Planning Commission
2023
Dinoflagellates are important components of marine ecosystems and essential coral symbionts, yet little is known about their genomes. We report here on the analysis a high-quality assembly from 1180-megabase genome Symbiodinium kawagutii. annotated protein-coding genes identified Symbiodinium-specific gene families. No whole-genome duplication was observed, but instead we found active (retro)transposition family expansion, especially in processes for successful symbiosis with corals. also...
Glyphosate is a globally popular herbicide to kill weeds and its wide applications may lead accumulation in coastal oceans as source of phosphorus (P) nutrient or growth inhibitor phytoplankton. We studied the physiological effects glyphosate on fourteen species representing five major phytoplankton phyla (haptophyta, bacillariophyta, dinoflagellata, raphidophyta, chlorophyta). Based responses different concentrations under contrasting dissolved inorganic (DIP) conditions, we found that...
Diatoms and dinoflagellates dominate coastal marine phytoplankton communities as major players of biogeochemical cycles their seasonal succession often leads to harmful algal blooms (HABs). What regulates respective dominances the development HABs remains elusive. Here we conducted time-sequential metatranscriptomic profiling on a natural assemblage that evolved from diatom dominance dinoflagellate bloom interrogate underlying metabolic ecological drivers. Data reveals similarity between...
Cefiderocol has been approved in the United States and Europe but not China. We aim to evaluate carbapenem-resistant Enterobacterales (CRE) susceptibility cefiderocol provide baseline data investigate resistance mechanism. From 2018 2019, 1,158 CRE isolates were collected from 23 provinces municipalities across The MICs of antimicrobials determined via agar dilution broth microdilution methods. Whole-genome sequencing was performed for 26 cefiderocol-resistant Escherichia coli Clone...
A new compound (1), named diaporthelactone, together with two known compounds (2 and 3) were isolated from the culture of Diaporthe sp., a marine fungus growing in submerged rotten leaves Kandelia candel mangrove nature conservation areas Fugong, Fujian Province China. The was elucidated to be 1,3-dihydro-4-methoxy-7-methyl-3-oxo-5-isobenzofuran-carboxyaldehyde which showed cytotoxic activity against KB Raji cell lines (IC50 6.25 5.51 microg mL(-1), respectively). Two compounds,...
The ability to utilize dissolved organic phosphorus (DOP) is important for phytoplankton survive the scarcity of inorganic (DIP), and alkaline phosphatase (AP) has been major research focus as a facilitating mechanism. Here, we employed unique molecular ecological approach conducted broader search underpinning mechanisms adenosine triphosphate (ATP) utilisation. Cultures dinoflagellate Karenia mikimotoi were set up in L1 medium (+P), DIP-depleted (-P) ATP-replacing-DIP (ATP). Differential...
Symbiodiniaceae community structure in corals is crucial for understanding the plasticity of different holobionts under environmental stress. While this relies on molecular analyses, accuracy quantification, as influenced by DNA extraction efficiency and rDNA copy number variations particular, has rarely been systematically investigated. Here, we report development a set genus-specific qPCR assays. First, protocol efficient isolation accurate measurements genome size was established. Second,...
NACs (NAM, ATAF1/2, and CUC2) are plant-specific transcription factors that play diverse roles in various plant developmental processes. In this study, we identified the NAC gene family birch (Betula pendula) further analyzed function of BpNACs. Phylogenetic analysis reveals 114 BpNACs can be divided into seven subfamilies. We investigated expression levels these different tissues including roots, xylem, leaves, flowers, results showed seem to expressed higher xylem roots than leaves...
Alkaline phosphatase (AP) in phytoplankton facilitates the utilization of dissolved organic phosphorus (DOP) when inorganic (DIP) is limited environment. The AP gene sequence and its expression under DIP limitation has not been studied dinoflagellates. In this study, we isolated full‐length cDNA from toxic dinoflagellate Amphidinium carterae Hulburt (2,112 bp, named as acaap ). deduced amino acid (ACAAP, 704 residues) was identified a membrane‐associated protein, agreement with dominantly...
The proton pump rhodopsin is widely found in marine bacteria and archaea, where it functions to capture light energy convert ATP. While several lineages of dinoflagellates, this gene has not been studied Prorocentrales species whether functionally tunes spectra intensities as remains unclear. Here we identified characterized the bloom-forming Prorocentrum donghaiense. It a 7-helix transmembrane polypeptide containing conserved domains critical amino acid residues PPR. This phylogenetically...
Cataloging an accurate functional gene set for the Symbiodiniaceae species is crucial addressing biological questions of dinoflagellate symbiosis with corals and other invertebrates. To improve models Fugacium kawagutii, we conducted high-throughput chromosome conformation capture (Hi-C) genome Illumina combined PacBio sequencing transcriptome to achieve a new assembly prediction. A 0.937-Gbp F. kawagutii were obtained, N50 > 13 Mbp longest scaffold 121 capped telomere motif at both ends....
Gene retroposition is an important mechanism of genome evolution but the role it plays in dinoflagellates, a critical player marine ecosystems, not known. Until recently, when genomes two coral-symbiotic dinoflagellate genomes, Symbiodinium kawagutii and S. minutum, were released, has been possible to systematically study these retrogenes. Here we examine abundant retrogenes (∼23% total genes) species. The hallmark presence DCCGTAGCCATTTTGGCTCAAG, spliced leader (DinoSL) constitutively...
Alkaline phosphatase (AP) is a key enzyme for phytoplankton to utilize dissolved organic phosphorus (DOP) when inorganic limited. While three major types of AP and their correspondingly diverse subcellular localization have been recognized in bacteria, little known about eukaryotic such as dinoflagellates. Here, we isolated full-length cDNA from latest-diverging dinoflagellate genus Alexandrium, conducted comparative analyses with homologs relatively basal (Amphidinium carterae)...
Alkaline phosphatase (AP) is a key enzyme that enables marine phytoplankton to scavenge phosphorus (P) from dissolved organic (DOP) when inorganic phosphate scarce in the ocean. Yet how AP gene has evolved phytoplankton, particularly dinoflagellates, poorly understood. We sequenced full-length genes and corresponding complementary DNA (cDNA) 15 strains (10 species), representing four classes of core dinoflagellate lineage, Gymnodiniales, Prorocentrales, Suessiales, Gonyaulacales....
Abstract Symbiodiniaceae dinoflagellates are essential endosymbionts of reef building corals and some other invertebrates. Information their genome structure function is critical for understanding coral symbiosis bleaching. With the rapid development sequencing technology, draft assemblies several species diverse marine algal genomes have become publicly available but spread in multiple separate locations. Here, we present a Algal Genomic Resource Database (SAGER), user-friendly online...
Free-living (FL) and particle-associated (PA) bacterioplankton communities play critical roles in biogeochemical cycles the ocean. However, their community composition, assembly process functions continental shelf slope regions are poorly understood. Based on 16S rRNA gene amplicon sequencing, we investigated bacterial communities' driving factors, processes functional potentials at a subtropical marginal sea. The showed specific distribution patterns with respect to lifestyle (free living...