A5017942895- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Marine and coastal ecosystems
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Adenosine and Purinergic Signaling
- Photoreceptor and optogenetics research
- Coral and Marine Ecosystems Studies
- Multiple Myeloma Research and Treatments
- Receptor Mechanisms and Signaling
- bioluminescence and chemiluminescence research
- Eosinophilic Disorders and Syndromes
- RNA modifications and cancer
- Neuropeptides and Animal Physiology
- Cancer Cells and Metastasis
- Light effects on plants
- Cellular transport and secretion
- Advanced Nanomaterials in Catalysis
- Genomics and Phylogenetic Studies
- Epigenetics and DNA Methylation
- Nitric Oxide and Endothelin Effects
- Chronic Myeloid Leukemia Treatments
- Neuroendocrine Tumor Research Advances
- Chemical Reactions and Isotopes
- Cancer, Stress, Anesthesia, and Immune Response
- Photosynthetic Processes and Mechanisms
Université de Montréal
2008-2024
Hôpital Maisonneuve-Rosemont
2024
Université de Sherbrooke
2019-2024
Dinoflagellates are important components of marine ecosystems and essential coral symbionts, yet little is known about their genomes. We report here on the analysis a high-quality assembly from 1180-megabase genome Symbiodinium kawagutii. annotated protein-coding genes identified Symbiodinium-specific gene families. No whole-genome duplication was observed, but instead we found active (retro)transposition family expansion, especially in processes for successful symbiosis with corals. also...
Dinoflagellates are an important component of the marine biota, but a large genome with high–copy number (up to 5,000) tandem gene arrays has made genomic sequencing problematic. More importantly, little is known about expression and conservation these unusual arrays. We assembled de novo catalog 74,655 contigs for dinoflagellate Lingulodinium polyedrum from RNA-Seq (Illumina) reads. The contains 93% EST dataset deposited in GenBank 94% enzymes 16 primary metabolic KEGG (Kyoto Encyclopedia...
Almost all cells display circadian rhythms, approximately 24-hour period changes in their biochemistry, physiology or behavior. These rhythms are orchestrated by an endogenous clock whose mechanism is based on transcription-translation feedback loops (TTFL) where the translated products of genes act to inhibit own transcription. We have used RNA-Seq measure abundance transcripts RNA-Seq-derived de novo gene catalog two different experiments. One compared midday and midnight a light–dark...
Dinoflagellates are important contributors to the marine phytoplankton and global carbon fixation, but also infamous for their ability form spectacular harmful algal blooms called red tides. While often associated with high available nitrogen, there instances where they observed in oligotrophic environments. In order maintain massive population conditions of nitrogen limitation, dinoflagellates must have evolved efficient adaptive mechanisms. Here we report physiological responses...
Cell migration is a ubiquitous process necessary to maintain and restore tissue functions. However, in cancer, cell leads metastasis development thus worsens the prognosis. Although mechanism of well understood, identification new targets modulating deciphering their signaling events could lead therapies functions diseases, such as inflammatory bowel disease, or block metastatic different forms cancer. Previous research has identified G-protein-coupled P2Y6 receptor an innovative target that...
In many phytoplankton species, cell division (mitosis) usually occurs at defined times of day. This timing is also observed under constant conditions, indicating that it regulated by a circadian clock rather than simple response to the light-dark cycle. For those algae with cycles longer day, opens window opportunity for mitosis particular time day through which cells in an appropriate phase cycle can pass. Although generally studied due ease measurement, some S-phase circadian. thus raises...
The control of transcription is poorly understood in dinoflagellates, a group protists whose permanently condensed chromosomes are formed without histones. Furthermore, while transcriptomes contain number proteins annotated as factors, the majority these cold shock domain which also known to bind RNA, meaning true factors unknown. Here we have assessed transcriptional response light photosynthetic species Symbiodinium kawagutii. We find that three genes previously reported respond using qPCR...
Dinoflagellates have been shown to express miRNA by bioinformatics and RNA blot (Northern) analyses. However, it is not yet known if miRNAs are able alter gene expression in this class of organisms. We assessed the possibility that may mediate circadian regulation dinoflagellate Lingulodinium polyedrum using Luciferin Binding Protein (LBP) as a specific example. LBP good candidate for since mRNA levels constant over daily cycle while protein synthesis restricted clock period several hours at...
Abstract Background The stem cell marker leucine-rich G-protein-coupled receptor-5 (LGR5) acts as an R-spondin (RSPO) signal transducer, facilitating WNT signaling to support tissue development and renewal. In GI diseases, LGR5+intestinal cells play a critical role in wound healing, while its expression by cancer correlates with progression chemoresistance. Surprisingly, only handful of studies have investigated the functional LGR5 receptor. Demonstrating receptor function identifying...
Abstract Background Leucine-rich G protein coupled receptor-5 (LGR5) is a GPCR originally identified as marker for intestinal stem cells but now associated to stemness in numerous other tissues. R-spondins (RSPO) are the only reported ligands of LGR5, which upon receptor binding, potentiates canonical Wnt/b-catenin pathway. Surprisingly, despite presence classical features such conserved DRY and NPXXY motifs RSPO binding LGR5 does not induce behaviors coupling heterotrimeric G-proteins or...