A5101816391- Respiratory viral infections research
- Pneumonia and Respiratory Infections
- Viral gastroenteritis research and epidemiology
- Bacterial Infections and Vaccines
- Antibiotic Resistance in Bacteria
- Plant Molecular Biology Research
- Insect Resistance and Genetics
- Viral Infections and Immunology Research
- Influenza Virus Research Studies
- Antibiotics Pharmacokinetics and Efficacy
- RNA modifications and cancer
- MicroRNA in disease regulation
- Vestibular and auditory disorders
- Amoebic Infections and Treatments
- Tuberculosis Research and Epidemiology
- Plant Pathogenic Bacteria Studies
- Biofuel production and bioconversion
- Parasitic infections in humans and animals
- Parasitic Infections and Diagnostics
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Animal Genetics and Reproduction
- Animal Virus Infections Studies
- Photosynthetic Processes and Mechanisms
Hospital of Hebei Province
2019-2025
Shandong University of Traditional Chinese Medicine
2023
Hebei Medical University
2019-2020
Sichuan University
2019
Shandong Academy of Agricultural Sciences
2018
Institute of Plant Protection
2018
Beijing Jiaotong University
2010
Shandong Agricultural University
2007-2009
Carbapenem-resistant Klebsiella pneumonia (CRKP) is a global threat that varies by region. The distribution, evolution, and clinical implications of the ST11 CRKP clone remain obscure. We conducted multicenter molecular epidemiological survey using isolates obtained from 28 provinces municipalities across China between 2011 2021. integrated sequences public databases performed genetic epidemiology analysis CRKP. Among CRKP, KL64 serotypes exhibited considerable expansion, increasing 1.54% to...
The increasing prevalence of severe Mycoplasma pneumoniae pneumonia (SMPP) poses a significant threat to the health children. This study aimed characterise and assess outcomes in children with SMPP.
Abstract Background Genes in the CCCH family encode zinc finger proteins containing motif with three cysteines and one histidine residues. They have been known to play important roles RNA processing as RNA-binding animals. To date, few plant studied functionally. Results In this study, a comprehensive computational analysis identified 68 67 genes Arabidopsis rice, respectively. A complete overview of gene was presented, including structures, phylogeny, protein motifs, chromosome locations....
* Zinc finger proteins are a superfamily involved in many aspects of plant growth and development. However, CCCH-type zinc stress tolerance poorly understood. A cDNA clone designated Gossypium hirsutum protein 1 (GhZFP1), which encodes novel protein, was isolated from salt-induced cotton (G. hirsutum) library using differential hybridization screening further studied transgenic tobacco Nicotiana tabacum cv. NC89. Using yeast two-hybrid (Y2H), GZIRD21A (GhZFP1 interacting responsive to...
The transcription factors C-repeat binding factors/dehydration-responsive element proteins (CBFs/DREBs) control the expression of many stress-inducible genes in Arabidopsis. A cDNA clone, designated GhDREB1, was isolated from cotton (Gossypium hirsutum) by library screening. Northern blot analysis indicated that mRNA accumulation GhDREB1 induced low temperatures and salt stress, but not abscisic acid (ABA) or drought stress seedlings. Transgenic tobacco (Nicotiana tabacum) plants...
Influenza virus is a contagious respiratory pathogen that can cause severe acute infections with long-term adverse outcomes. For paediatric patients at high risk of influenza, the readmission and associated factors remain unclear. Children discharged diagnosis or critical influenza from October 2021 to March 2022 were included. The disease severity was categorized according "2020 edition Chinese expert consensus on treatment in children". Demographic data, clinical characteristics,...
Blastocystis sp. is a common enteric protist that colonizes humans and wide range of animals. Although some studies have reported incidences in animals China, there are limited data available concerning the prevalence among people non-human primates. The aims present study were to determine prevalence, subtype distribution genetic characteristics primates, investigate potential for zoonotic transmission between human A total 185 faecal samples from primates 1,118 volunteers collected Hebei...
The most prevalent viruses currently causing diarrhea are norovirus and rotavirus, rapid sensitive detection methods essential for the early diagnosis of disease. purpose this study was to establish a single-tube two-stage nucleic acid amplification method-reverse transcription recombinase-assisted PCR (RT-RAP)-for simultaneous GII group A Rotavirus, with first stage consisting isothermal reverse recombinase-aided (RT-RAA) second qPCR (quantitative PCR). RT-RAP is more than either RT-RAA or...
Abstract Background Acute respiratory infections (ARI) are a threat to human health and survival, resulting in many paediatric hospitalisations. However, the epidemiological clinical severity characteristics of parainfluenza virus (PIV), one most prevalent viruses, not well understood children. Methods To identify features PIV infection, 2019, hospitalised children with ARI were screened using multiplex polymerase chain reaction (PCR) for 10 other common pathogens. Subtyping randomly...
Abstract Background In this retrospective study, we aimed to evaluate the factors associated with severity of respiratory syncytial virus (RSV) bronchiolitis in children aged under 2 years who were admitted Children’s Hospital Hebei between June 2018 and January 2019. Methods Sputum samples positive for RSV via multiplex PCR subtyped using real-time PCR. Data collected included risk disease severity, demographics, microbiology, outcomes. Results Of 82 bronchiolitis, 79 treated discharged...
Hypervirulent carbapenem-resistant Klebsiella pneumoniae (hv-CRKp) poses a significant threat to public health. This study reports an infection related hv-CRKp in premature infant and reveals its colistin resistance evolutionary mechanisms within the host. Three KPC-producing CRKp strains were isolated from patient with sepsis osteoarthritis who had been receiving antimicrobial therapy. The minimum inhibitory concentrations (MICs) of...
Energy efficiency and data reliability have always been the key problem in WSN. In this paper, firstly, a selection of cluster head algorithm is designed which able to detect energy change nodes, rapidly ascertain new head, avoid excessive consumption between nodes. Secondly, differentiated service introduced our makes achieve higher reliability. Finally, evaluating consumption, simulation results show that it obtains reliability, simultaneously achieves lower consumption.
Cell walls play an important role in the structure and morphology of plants as well responses to various biotic abiotic stresses. Although comprehensive analysis genes involved cellulose synthase has been performed model plants, such Arabidopsis thaliana rice, information regarding synthase-like (Csl) maize is limited. In this study, a total 56 members Csl gene family were identified genome classified into six subfamilies. Analysis conserved motif indicated functional similarities among...
Abstract Background Community-acquired pneumonia (CAP) is usually diagnosed in children, and the type of respiratory specimen critical. Differences pathogens detection between induced sputum (IS) bronchoalveolar lavage fluid (BALF) have not been evaluated. Methods In 2018, paired BALF samples from CAP hospitalised children with indications for (BAL) were subjected to multiplex PCR 11 common pathogens. Results A total 142 tested. The overall positivity rate was 85.9% (122/142) 80.3% (114/142)...
Transgenic maize hybrids that express the Aspergillus niger phyA2 gene could significantly improve phosphorus bioavailability to poultry and livestock. However, little information has been reported about effects of phytase transgenic on Asian corn borer (ACB), Ostrinia furnacalis (Guenée). This study provides valuable physiological, biochemical gut microflora functional diversity changes ACBs fed maize. The weights, survival rates, in vivo protein contents, activities two detoxification...
Febrile seizures (FS) are a common cause of paediatric emergencies, but research on their aetiology and epidemiology limited. The aim this study was to investigate the prevalence central nervous system (CNS) pathogenic infections in patients with FS-associated hospitalization.A prospective observational conducted children under 16 years age hospitalization. Demographic, clinical laboratory data were recorded. Multiplex-PCR performed cerebrospinal fluid (CSF) samples for nine viruses,...
Introduction: Multiplex PCR methods have significantly improved the diagnosis of acute respiratory tract infections (ARTIs) in children. The ResP-CE System coupled with capillary electrophoresis is a highly specialized, automated, and expensive technology for detecting common pathogens ARTIs. XYRes-MCA System, remarkably less multiplex instrument, employs hybridization detection ARTI pathogens. Both detect 9 microorganisms ARTIs, i.e., RSV, FLUAV, FLUBV, ADV, PIV, HMPV, HBOV, HCOV, MP. In...
Metagenomic next-generation sequencing (mNGS) is useful for difficult to cultivate pathogens. Here, we use cerebrospinal fluid mNGS diagnose invasive meningococcal disease. The complete genome sequences of Neisseria meningitidis were assembled using N. ST4821-serotype C isolated from four patients. To investigate the phylogeny, 165 CC4821 genomes 1972 2017 also included. core accumulated variation at a rate 4.84×10-8 substitutions/nucleotide site/year. differentiated into sub-lineages during...
Abstract Background Viral encephalitis is common in childhood. It an acute brain parenchymal inflammation caused by a variety of viral infection, and enterovirus accounts for the majority. Due to atypical clinical manifestations, pathogenic testing important assisting diagnosis. The purpose this study was evaluate performance multiplex PCR assay compared with quantitative real‐time detection. Methods A prospective case‐control performed involving 103 pediatric patients suspected...