Zoo animals, harboring zoonotic gastrointestinal protozoal diseases, pose potential hazards to the safety of visitors and animal keepers. This study involved collection examination 400 fresh fecal samples from 68 species, obtained five zoos. The aim this was determine occurrence, genetic characteristics, common protists. PCR or nested analysis conducted on these detect four specific parasites: Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi, Blastocystis spp. overall...

Toxoplasma gondii is a major opportunistic pathogen that spreads in range of animal species and human beings. Quite few characterizations apoptosis have been identified T. treated with inducers, but the molecular mechanisms pathway are not clearly understood. Metacaspases caspase-like cysteine proteases can be found plants, fungi, protozoa which caspases absent. multifunctional involved apoptosis-like cell death, insoluble protein aggregate clearance, proliferation. To investigate whether...

 Gully erosion represents one of the most severe forms land degradation. In regional management decisions, gully density serves as a crucial metric. As an essential measure under China’s national strategy for protecting black soil, control projects rely on accurate simulation at scale to enable more efficient and precise management. Taking northeast Songnen typical soil region study area, this research employed stratified unequal probability systematic sampling method...

Blastocystis sp. is a common enteric protist that colonizes humans and wide range of animals. Although some studies have reported incidences in animals China, there are limited data available concerning the prevalence among people non-human primates. The aims present study were to determine prevalence, subtype distribution genetic characteristics primates, investigate potential for zoonotic transmission between human A total 185 faecal samples from primates 1,118 volunteers collected Hebei...

Introduction Candida glabrata is a prevalent opportunistic fungal pathogen in humans, and fluconazole (FLC) one of the most commonly used antifungal agents. However, molecular mechanisms underlying FLC tolerance C. remain largely unexplored. Objective This study aims to identify novel regulating tolerance, with particular focus on tunicamycin (TUN)-induced respiratory deficiency. Methods We employed three distinct experimental approaches investigate impact TUN tolerance: (1) co-treatment...

Neospora caninum, of the Apicomplexa phylum, is a common cause abortions in cattle and nervous system dysfunction dogs. Rhoptry proteins play an important role virulence. The objectives this study were to functions NcROP5 N. caninum by deleting gene from wild Nc-1 strain. We selected ToxoDB successfully constructed gene-deleted vector, pTCR-NcROP5-CD KO. Then we screened knockout strains (ΔNcROP5) at gene, protein transcription levels. Plaque assay, host cell invasion assay intracellular...

Blastocystis sp. is a kind of protozoa living in the intestinal tract human and animals, which will cause diseases such as diarrhea, abdominal distension vomiting. This paper was aimed to understand infection In golden monkeys transmission path North China. Thirty-seven feces samples from 116 cockroach Shijiazhuang Zoo were collected July October 2019 for PCR analysis Genetic diversity further conducted on with positive results. The results showed that rate 48.7% (18/37) 82.8% (96/116)...

Blastocystis sp. is a zoonotic unicellular eukaryote that distributed worldwide. The pathogenicity of has been debated over the years. In this study, mice were infected with ST2 to assess impact and underlying mechanisms on host by integrating transcriptomics, metabolomics, gut microbiomes. Transcriptomic analysis revealed significant differences in expression genes related inflammatory cytokines, tumors, neuropathic disease‐related factors parasite. A total 430 differentially expressed...

The development of phosphorylation-suppressing inhibitors targeting Signal Transducer and Activator Transcription 3 (STAT3) represents a promising therapeutic strategy for non-small cell lung cancer (NSCLC). In this study, generative model was developed using transfer learning virtual screening, leveraging comprehensive dataset STAT3 to explore the chemical space novel candidates. This approach yielded chemically diverse library compounds, which were prioritized through molecular docking...

Nonvolatile favor compounds are important for icewine mouthfeel and flavor. Three tiers (ranks) of Vidal icewines from vintages 2008 to 2010 in China were selected organic acid, amino phenolic compound analysis by HPLC UPLC, a large database on nonvolatile flavor was developed. The results revealed similar distribution profiling among the nine samples. most abundant acids L-malic, tartaric, acetic, citric, principal arginine proline. Hydroxybenzoic acid derivatives major constituents...

Blastocystis sp. is a eukaryotic protozoan parasite reported in patients with diarrhea and irritable bowel syndrome. So far, it has been determined the occurs intestinal tract of humans animals worldwide. However, few studies have investigated distribution cold-water fish cultured water. After DNA extraction tissue water samples, polymerase chain reaction (PCR) assay was established to detect The results showed detection rate 3.70% samples 6.29% samples. Only ST1 subtype fish, but five...

Toxoplasma gondii (T. gondii) and Neospora caninum (N. caninum) are protozoan parasites that cause severe disease in animals significant economic losses for farmers worldwide. is considered to be a serious parasite dairy cows, while T. infection parasitic can infect people livestock. The aim of the present study was investigate seroprevalence N. cows Hebei province, China. A total 723 cow sera samples from three regions were analyzed by enzyme-linked immunosorbent assays (ELISA). results...

Lung adenocarcinoma is the most prevalent subtype of lung cancer, which leading cause cancer-related mortality worldwide. Toxoplasma gondii (T.gondii) Rhoptry protein 16 (ROP16) has been shown to quickly enter nucleus, and through activate host cell signaling pathways by phosphorylation STAT3 may affect survival tumor cells. This study constructed recombinant lentiviral expression vector T. ROP16 I/II/III stably transfected them into A549 cells, effects on proliferation, cycle, apoptosis,...

<title>Abstract</title> The discovery of phosphorylation-suppressed inhibitors for Signal Transducer and Activator Transcription 3 (STAT3) presents a novel therapeutic strategy non-small cell lung cancer (NSCLC). Despite the pivotal roles STAT3 in progression, effective remain limited, especially efficiently suppressing phosphorylation at Try705. This study harnesses generative deep learning to develop model de novo design that selectively target phosphorylated form subsequentially induce...

Blastocystis sp. is a unicellular protozoan and one of the most common gastrointestinal eukaryotic parasites in humans animals. It distributed worldwide, but its pathogenic role still controversial. subdivided into 33 subtypes, existing different subtypes imply host specificity various species. The study aimed to investigate detail range sp., given limited studies. A total 720 fecal samples from 132 animal species, 140 (19.44%) were PCR positive for with 43.67% (31/71), 5.89% (7/119), 34.87%...