A5101664916- Pancreatic and Hepatic Oncology Research
- Cancer Genomics and Diagnostics
- Neuroendocrine Tumor Research Advances
- Pancreatitis Pathology and Treatment
- Alkaline Phosphatase Research Studies
- Bone health and treatments
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Cholangiocarcinoma and Gallbladder Cancer Studies
- MicroRNA in disease regulation
- Renal cell carcinoma treatment
- Metalloenzymes and iron-sulfur proteins
- RNA modifications and cancer
- Cancer Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- ATP Synthase and ATPases Research
- Neuroblastoma Research and Treatments
- Click Chemistry and Applications
- Gallbladder and Bile Duct Disorders
- Lung Cancer Research Studies
- Ferroptosis and cancer prognosis
- Circular RNAs in diseases
- Cancer Diagnosis and Treatment
- Cancer Cells and Metastasis
Peking Union Medical College Hospital
2015-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2015-2024
Shandong Geological Sciences Institute
2024
Academy of Medical Sciences
2014-2023
Nanjing Medical University
2022
Jiangsu Province Hospital
2022
Fudan University Shanghai Cancer Center
2021
Imperial College London
2020
Tsinghua University
2020
Christie's
2016
DNA modifications such as 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are epigenetic marks known to affect global gene expression in mammals. Given their prevalence the human genome, close correlation with high chemical stability, these could serve ideal biomarkers for cancer diagnosis. Taking advantage of a highly sensitive selective labeling technology, we report here genome-wide profiling 5hmC circulating cell-free (cfDNA) genomic (gDNA) paired tumor adjacent tissues...
Chemoresistance is one of the main causes poor prognosis in pancreatic cancer patients. Understanding mechanisms implicated chemoresistance critical to improving patient outcomes. Recent evidences indicate that long noncoding RNAs (lncRNAs) are involving cancer. However, lncRNAs contribute resistance and remain largely unknown. The objective this study construct a chemoresistance-related lncRNA-associated competing endogenous RNA (ceRNA) network identify key regulating network.Firstly,...
Background: Pancreatic cancer is highly lethal and aggressive with increasing trend of mortality in both genders. An effective prediction model needed to assess prognosis patients for optimization treatment. Materials methods: Seven datasets mRNA expression clinical data were obtained from gene omnibus (GEO) database. Level 3 clinicopathological The Cancer Genome Atlas pancreatic ductal adenocarcinoma (TCGA‑PAAD) dataset. Differentially expressed genes (DEGs) between tumor normal tissue...
Self-complementary adeno-associated virus (scAAV) vectors can significantly minimize the vector load required to achieve sustained transgene expression. In this study, transcriptional regulatory elements were systematically screened produce constitutive and liver-specific scAAV factor IX (FIX) expression cassettes. addition, optimization of GC content, cis- elements, codon usage in human FIX (hFIX) increased 4–20-fold. A was developed that capable expressing high levels comparison with...
Objectives The aim of this study was to determine the etiology tendency acute pancreatitis (AP) in Beijing region and relationship with influencing factors. Methods This retrospective multicenter enrolled 8 representative general hospitals from January 1, 2006 December 31, 2010. analyzed, defined sex, aging, severity, mortality, recrudesce rate, length stay, hospitalization cost. Results 2461 patients. total number increasing year by year. Causes included biliary (1372, 55.75%), alcoholism...
Reprogrammed energy metabolism has become an emerging hallmark of cancer in recent years. Transporters have been reported to be amino acid sensors involved controlling mTOR recruitment and activation, which is crucial for the growth both normal tumor cells. L-type transporter 2 (LAT2), encoded by SLC7A8 gene, a Na+-independent neutral responsible transporting acids, including glutamine, can activate mTOR. Previous studies shown that LAT2 was overexpressed gemcitabine-resistant pancreatic...
Insulinomas and non-functional pancreatic neuroendocrine tumours (NF-PanNETs) have distinctive clinical presentations but share similar pathological features. Their genetic bases not been comprehensively compared. Herein, we used whole-genome/whole-exome sequencing (WGS/WES) to identify differences between insulinomas NF-PanNETs.The mutational profiles copy-number variation (CNV) patterns of 211 PanNETs, including 84 127 NF-PanNETs, were obtained from WGS/WES data provided by Peking Union...
Abstract Purpose: Gemcitabine is most commonly used for pancreatic cancer. However, the molecular features and mechanisms of frequently occurring resistance remain unclear. This work aims at exploring gemcitabine identifying candidate biomarkers combinatorial targets treatment. Experimental Design: In this study, we established 66 patient-derived xenografts (PDXs) on basis clinical cancer specimens treated them with gemcitabine. We generated multiomics data (including whole-exome sequencing,...
Chemoresistance is one of the causes poor prognosis in pancreatic cancer patients. However, mechanisms resistance remain unclear. Here we screened miRNAs associated with drug cancer, and identified a panel dysregulated gemcitabine-resistance cells, including 13 downregulated 20 upregulated miRNAs. Further studies focusing on miR-497 demonstrated that suppressed cells proliferation, decreased percentage S phase re-sensitized to gemcitabine erlotinib, attenuated migration invasion capacities....
Objectives: To identify plasma microRNA (miRNA) markers of pancreatic cancer (PC). Background: Accurate pretreatment diagnosis PC remains challenging, whether miRNAs could be used as biomarkers in unknown. Methods: In this multiphase multicenter study, peripheral blood samples were obtained preoperatively 3 phases: the discovery phase [7 patients with PC, 6 chronic pancreatitis (CP), and 5 healthy volunteers (N)], preliminary validation (29 16 CP, 31 N), large sample (156 65 N, 57 27...
Abstract Background By regulating target genes, microRNAs play essential roles in carcinogenesis and drug resistance human pancreatic ductal adenocarcinoma (PDAC). Previous studies have shown that microRNA-10a-5p (miR-10a-5p) is overexpressed PDAC acts as an oncogene to promote the metastatic behavior of cells. However, role miR-10a-5p chemoresistance remains unclear. Methods The effects on biological behaviors were analyzed. MiR-10a-5p TFAP2C levels tissues detected, clinical value was...