A5102026349- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Analytical Chemistry and Chromatography
- Metabolomics and Mass Spectrometry Studies
- Cancer Genomics and Diagnostics
- RNA and protein synthesis mechanisms
- Advanced biosensing and bioanalysis techniques
- X-ray Diffraction in Crystallography
- Covalent Organic Framework Applications
- Cancer-related gene regulation
- Metal-Organic Frameworks: Synthesis and Applications
- Crystallization and Solubility Studies
- Mass Spectrometry Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Analytical Chemistry and Sensors
- Lung Cancer Treatments and Mutations
- Phytochemistry and Biological Activities
- Cancer Immunotherapy and Biomarkers
- Carbon Nanotubes in Composites
- Lung Cancer Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- PI3K/AKT/mTOR signaling in cancer
- Biosensors and Analytical Detection
- Advanced Chemical Sensor Technologies
- Gold and Silver Nanoparticles Synthesis and Applications
University of Louisville
2018-2025
Affiliated Hospital of Shandong University of Traditional Chinese Medicine
2024
National Institute of Measurement and Testing Technology
2022-2024
Chinese PLA General Hospital
2023
Beijing National Laboratory for Molecular Sciences
2009-2021
Peking University
2009-2021
Anhui Provincial Hospital
2021
Anhui Medical University
2021
Institute of Bioorganic Chemistry
2016-2019
Ludwig Cancer Research
2018-2019
Graphene is a monolayer of carbon atoms packed into two-dimensional (2D) honeycomb crystal structure, which special material with many excellent properties. In the present study, we will discuss possibility that graphene can be used as substrate for enhancing Raman signals adsorbed molecules. Here, phthalocyanine (Pc), rhodamine 6G (R6G), protoporphyin IX (PPP), and violet (CV), are popular molecules widely probe, deposited equally on SiO2/Si using vacuum evaporation or solution soaking. By...
We have measured resonance Raman spectra with greatly suppressed fluorescence (FL) background from rhodamine 6G (R6G) and protoporphyrin IX (PPP) adsorbed on graphene. The FL suppression is estimated to be ∼103 times for R6G. successful observation of peaks demonstrates that graphene can used as a substrate suppress in spectroscopy (RRS), which has potential applications low-concentration detection RRS study fluorescent molecules.
DNA modifications such as 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are epigenetic marks known to affect global gene expression in mammals. Given their prevalence the human genome, close correlation with high chemical stability, these could serve ideal biomarkers for cancer diagnosis. Taking advantage of a highly sensitive selective labeling technology, we report here genome-wide profiling 5hmC circulating cell-free (cfDNA) genomic (gDNA) paired tumor adjacent tissues...
The development of photocatalysts based on covalent organic frameworks (COFs) is intriguing research due to their structural flexibility and tremendous catalytic sites. Herein we demonstrate a facile strategy prepare composite materials combined COFs inorganic semiconductors with enhanced photocatalytic performances. A new photocatalyst (BiOBr/CTF-3D) integrated three-dimensional (3D) triazine framework (CTF-3D) two-dimensional BiOBr nanoflake was prepared via simple coprecipitation method....
The universally conserved Kae1/Qri7/YgjD and Sua5/YrdC protein families have been implicated in growth, telomere homeostasis, transcription the N6-threonylcarbamoylation (t6A) of tRNA, an essential modification required for translational fidelity by ribosome. In bacteria, YgjD orthologues operate concert with bacterial-specific proteins YeaZ YjeE, whereas archaeal eukaryotic systems, Kae1 operates as part a larger macromolecular assembly called KEOPS Bud32, Cgi121, Gon7 Pcc1 subunits. Qri7...
5-Methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC), two of the best-studied DNA modifications, play crucial roles in normal development disease mammals. Although 5-methylcytidine (m5C) 5-hydroxymethylcytidine (hm5C) have also been identified RNA, their distribution biological function RNA remain largely unexplored, due to lack suitable sequencing methods. Here, we report a base-resolution method for hm5C RNA. We applied selective oxidation trihydroxylated-thymine (thT) mediated by...
A highly specific enrichment method for N-linked glycopeptides was successfully developed using a hydrophilic covalent-organic framework with excellent binding capacity, stability and reusability.
Ethanol (EtOH)-induced alterations in intestinal homeostasis lead to multi-system pathologies, including liver injury. ω-6 PUFAs exert pro-inflammatory activity, while ω-3 promote anti-inflammatory activity that is mediated, part, through specialized pro-resolving mediators [e.g., resolvin D1 (RvD1)]. We tested the hypothesis a decrease ω-6:ω-3 PUFA ratio would attenuate EtOH-mediated gut-liver axis. FA desaturase-1 (fat-1) mice, which endogenously increase levels, were protected against...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is intricately related to the reprogramming of host metabolism. However, existing studies have mainly focused on peripheral blood samples and barely identified specific metabolites that are critically involved in pathology disease 2019 (COVID-19). In current small-scale study, we performed metabolic profiling plasma (n = 61) paired bronchoalveolar lavage fluid (BALF) 20) using parallel two-dimensional liquid...
Interconversion of white and brown adipocytes occurs between anabolic catabolic states. The molecular mechanism regulating this phenotypic switch remains largely unknown. This study explores the role tuberous sclerosis complex 1 (TSC1)–mechanistic target rapamycin (mTOR) signaling in conversion to adipose tissue (WAT). A colony Fabp4-Tsc1−/− mice, which Tsc1 gene was specifically deleted by fatty acid binding protein 4 (FABP4)-Cre, established. Western blotting immunostaining demonstrated...
Obesity is the accumulation of adipose tissue caused by excess energy in body, accompanied long-term chronic low-grade inflammation tissue. More than 50% interstitial cells are macrophages, which produce cytokines closely related to insulin resistance. Macrophage biology driven two polarization phenotypes, M1 (proinflammatory) and M2 (anti-inflammatory). This study aimed investigate effect gastric hormone des-acyl ghrelin (DAG) on phenotype macrophages elucidate role sensitivity its...
An ultrasensitive sheathless capillary electrophoresis–electrospray ionization–mass spectrometry system is developed for the determination of 5′-mdC and 5′-hmdC in only 125 pg (∼20 cells) genomic DNA samples.
Procalcitonin (PCT) is a potentially specific early marker of bloodstream infection and sepsis.
A novel strategy utilizing a DNA template-directed CuAAC click reaction to mimic ligation based on ligase was successfully established for multiple SNP detection with high sensitivity and specificity.
A simple and novel reporter-triggered isothermal exponential amplification reaction (R-EXPAR) integrated with a miniaturized electrochemical device was developed, which achieved excellent improvement (five orders of magnitude) sensitivity toward reporter, G-quadruplex. This R-EXPAR strategy has been successfully implemented to construct homogeneous label-free sensor for ultrasensitive DNA detection.
A facile and rapid postsynthetic modification strategy for functionalization of covalent organic framework (COF) was developed to synthesize a tailor-made pH-responsive COF called TpPa-1@Au@GSH highly efficient extraction N1-methyladenosine (m1A). Glutathione (GSH) judiciously designed as the functional group extracting releasing m1A by pH variations. With aid gold nanoparticles (Au NPs) linkers, GSH successfully introduced robust substrate TpPa-1 in only one step spending 1 h. Owing...
Lipids play a significant role in life activities and participate the biological system through different pathways. Although comprehensive two-dimensional liquid chromatography–mass spectrometry (2DLC-MS) has been developed to profile lipid abundance changes, identification quantification from 2DLC-MS data remain challenge. We created Lipid Wizard, open-source software for assignment isotopic peak stripping of data. Wizard takes list deconvoluted as input assigns each lipids recorded LIPID...
A label-free ultrasensitive method was established for the simultaneous determination of RNA modified nucleotides based on a sheathless capillary electrophoresis–tandem mass spectrometry system and successfully applied to investigate effects exposure nickel ions epigenetics.
The diverse characteristics and large number of entities make metabolite separation challenging in metabolomics. To date, there is not a singular instrument capable analyzing all types metabolites. In order to achieve better for higher peak capacity accurate identification quantification, we integrated GC × GC-MS parallel 2DLC-MS analysis polar test the performance developed system, 13 rats were fed different diets form two animal groups. Polar metabolites extracted from rat livers analyzed...
Abstract Although DNA 5-hydroxymethylcytosine (5hmC) is recognized as an important epigenetic mark in cancer, its precise role lymph node metastasis remains elusive. In this study, we investigated how 5hmC associates with breast cancer. Accompanying high expression of TET1 and TET2 proteins, large numbers genes the metastasis-positive primary tumors exhibit higher levels than those metastasis-negative tumors. contrast, TET protein decrease significantly within metastatic lesions nodes...
Modified cytosines are important epigenetic marks that exert critical influences in a variety of cellular processes living organisms. However, biological functions rare modified cytosines, especially certain 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC), still unclear due to the extremely low abundance samples. In this work, series novel hydrazine-based reagents, which held hydrazine group as reaction group, hydrophobic triazine two easily charged tertiary amine groups with different...