A5100368912- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Advanced Chemical Sensor Technologies
- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Diet and metabolism studies
- Immune cells in cancer
- Gut microbiota and health
- Extracellular vesicles in disease
- Cancer, Hypoxia, and Metabolism
- RNA modifications and cancer
- Diet, Metabolism, and Disease
- Trace Elements in Health
- Bioinformatics and Genomic Networks
- Spectroscopy and Chemometric Analyses
- Insect Resistance and Genetics
- Immune Cell Function and Interaction
- Cancer, Lipids, and Metabolism
- Gene expression and cancer classification
- Tryptophan and brain disorders
- Systemic Lupus Erythematosus Research
- Eicosanoids and Hypertension Pharmacology
- Biochemical effects in animals
University of Louisville
2016-2025
Yangzhou University
2019-2024
University of Louisville Hospital
2011-2024
Chinese People's Liberation Army
2024
Center for Environmental Health
2023
Chinese University of Hong Kong
2023
Karolinska Institutet
2022
Qinghai University
2021
Capital Medical University
2021
Zhejiang Cancer Hospital
2021
One of the defining characteristics a pre-metastatic niche, fundamental requirement for primary tumor metastasis, is infiltration immunosuppressive macrophages. How these macrophages acquire their phenotype remains largely unexplored. Here, we demonstrate that tumor-derived exosomes (TDEs) polarize toward an characterized by increased PD-L1 expression through NF-kB-dependent, glycolytic-dominant metabolic reprogramming. TDE signaling TLR2 and NF-κB leads to glucose uptake. TDEs also...
Cholestatic liver disease is characterized by gut dysbiosis and excessive toxic hepatic bile acids (BAs). Modification of microbiota repression BA synthesis are potential strategies for the treatment cholestatic disease. The purpose this study was to examine effects understand mechanisms probiotic Lactobacillus rhamnosus GG (LGG) on synthesis, injury, fibrosis in duct ligation (BDL) multidrug resistance protein 2 knockout (Mdr2-/- ) mice.Global intestine-specific farnesoid X receptor (FXR)...
Abstract High-fat diet (HFD) decreases insulin sensitivity. How high-fat causes resistance is largely unknown. Here, we show that lean mice become resistant after being administered exosomes isolated from the feces of obese fed a HFD or patients with type II diabetes. altered lipid composition predominantly phosphatidylethanolamine (PE) in animals (L-Exo) to phosphatidylcholine (PC) (H-Exo). Mechanistically, intestinal H-Exo taken up by macrophages and hepatocytes, leading inhibition...
Lung inflammation is a hallmark of coronavirus disease 2019 (COVID-19). In this study, we show that mice develop inflamed lung tissue after being administered exosomes released from the epithelial cells exposed to severe acute respiratory syndrome 2 (SARS-CoV-2) Nsp12 and Nsp13 (exosomesNsp12Nsp13). Mechanistically, exosomesNsp12Nsp13 are taken up by macrophages, leading activation nuclear factor κB (NF-κB) subsequent induction an array inflammatory cytokines. Induction tumor necrosis...
Plant exosomes protect plants against infection; however, whether edible plant can mammalian hosts infection is not known. In this study, we show that ginger exosome-like nanoparticles (GELNs) are selectively taken up by the periodontal pathogen Porphyromonas gingivalis in a GELN phosphatidic acid (PA) dependent manner via interactions with hemin-binding protein 35 (HBP35) on surface of P. gingivalis. Compared PA (34:2), (34:1) did interact HBP35, indicating degree unsaturation plays...
Macrophages have been linked to tumor initiation, progression, metastasis, and treatment resistance. However, the transcriptional regulation of macrophages driving protumor function remains elusive. Here, we demonstrate that transcription factor c-Maf is a critical controller for immunosuppressive macrophage polarization in cancer. controls many M2-related genes has direct binding sites within conserved noncoding sequence Csf-1r gene promotes M2-like macrophage–mediated T cell suppression...
Gut microbiota function has numerous effects on humans and the diet consume emerged as a pivotal determinant of gut function. Here, new concept that can be trained by diet-derived exosome-like nanoparticles (ELNs) to release healthy outer membrane vesicles (OMVs) is introduced. Specifically, OMVs released from garlic ELN (GaELNs) human Akkermansia muciniphila (A. muciniphila) reverse high-fat diet-induced type 2 diabetes (T2DM) in mice. Oral administration GaELNs A. traffick brain where they...
Abstract Diet has emerged as a key impact factor for gut microbiota function. However, the complexity of dietary components makes it difficult to predict specific outcomes. Here we investigate plant-derived nanoparticles (PNP) on and metabolites in context cancer immunotherapy with humanized gnotobiotic mouse model. Specifically, show that ginger-derived exosome-like nanoparticle (GELN) preferentially taken up by Lachnospiraceae Lactobacillaceae mediated digalactosyldiacylglycerol (DGDG)...
Alcohol consumption induces liver steatosis; therefore, this study investigated the possible role of adipose tissue dysfunction in pathogenesis alcoholic steatosis. Mice were pair-fed an alcohol or control liquid diet for 8 weeks to evaluate effects on lipid metabolism at tissue-liver axis. Chronic exposure reduced mass and adipocyte size. Fatty acid release from explants was significantly increased alcohol-fed mice association with activation triglyceride lipase hormone-sensitive lipase....
Background Comprehensive two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GCxGC-TOF MS) has been proposed as a powerful new tool for multidimensional analysis of complex chemical mixtures. We investigated GCxGC-TOF MS method identifying volatile organic compounds (VOCs) in normal human breath. Methods Samples alveolar breath VOCs and ambient room air VOC were collected collection apparatus (BCA) onto separate sorbent traps from 34 healthy volunteers (mean age...
Diet and bile play critical roles in shaping gut microbiota, but the molecular mechanism underlying interplay with intestinal microbiota is unclear. Here, we showed that lemon-derived exosome-like nanoparticles (LELNs) enhance lactobacilli toleration to bile. To decipher mechanism, used Lactobacillus rhamnosus GG (LGG) as proof of concept show LELNs LGG resistance via limiting production Msp1 Msp3, resulting decrease accessibility cell membrane. Furthermore, found decline Msps protein levels...
Immune checkpoint inhibitor (ICI) therapy is effective against many cancers for a subset of patients; large percentage patients remain unresponsive to this therapy. One contributing factor ICI resistance accumulation monocytic myeloid-derived suppressor cells (M-MDSCs), innate immune with potent immunosuppressive activity T lymphocytes. Here, using lung, melanoma, and breast cancer mouse models, we show that CD73-expressing M-MDSCs in the tumor microenvironment (TME) exhibit superior cell...
Objectives Zinc (Zn) deficiency often occurs in the patients with diabetes. Effects of Zn on diabetes-induced hepatic injury were investigated. Methods Type 1 diabetes was induced FVB mice multiple low-dose streptozotocin. Hyperglycemic and age-matched control treated without chelator, N,N,N′,N′-tetrakis (2-pyridylemethyl) ethylenediamine (TPEN), at 5 mg/kg body-weight daily for 4 months. Hepatic examined by serum alanine aminotransferase (ALT) level liver histopathological biochemical...
A novel peak alignment algorithm using a distance and spectrum correlation optimization (DISCO) method has been developed for two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC/TOF-MS)-based metabolomics. This uses the output of instrument control software, ChromaTOF, as its input data. It detects merges multiple entries same metabolite into one entry in each list. After z-score transformation retention times, DISCO selects landmark peaks from all samples based on...
A method of employing high-resolution mass spectrometry in combination with vivo metabolite deuterium labeling was developed this study to investigate the effects alcohol exposure on lipid homeostasis at white adipose tissue (WAT)-liver axis a mouse model alcoholic fatty liver. In order differentiate liver lipids synthesized from acids that were transported back and other sources acids, two-stage feeding experiment performed incorporate into metabolites. Hepatic extracted liver, epididymal...
The interactions among the gut, liver, and immune system play an important role in liver disease. Probiotics have been used for treatment prevention of many pathological conditions, including diseases. Comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GC×GC-TOF MS) was herein, conjunction with chemometric data analysis, to identify metabolites significantly affected by probiotics mice fed or without alcohol. metabolomics analysis indicates that levels fatty...