A5091695182- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Microfluidic and Bio-sensing Technologies
- Single-cell and spatial transcriptomics
- 3D Printing in Biomedical Research
- Immune cells in cancer
- HIV, Drug Use, Sexual Risk
- HIV/AIDS Research and Interventions
- Biliary and Gastrointestinal Fistulas
- Gallbladder and Bile Duct Disorders
- Innovative Microfluidic and Catalytic Techniques Innovation
- MicroRNA in disease regulation
- Sex work and related issues
- RNA Interference and Gene Delivery
- Adolescent Sexual and Reproductive Health
- Adenosine and Purinergic Signaling
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Ultrasound and Cavitation Phenomena
- Esophageal and GI Pathology
- Microbial Inactivation Methods
University of Louisville
2017-2025
University of California, San Diego
2024
San Diego State University
2024
Florida State University
2024
University of North Carolina at Chapel Hill
2023-2024
James Graham Brown Foundation
2022-2023
Immune checkpoint inhibitor (ICI) therapy is effective against many cancers for a subset of patients; large percentage patients remain unresponsive to this therapy. One contributing factor ICI resistance accumulation monocytic myeloid-derived suppressor cells (M-MDSCs), innate immune with potent immunosuppressive activity T lymphocytes. Here, using lung, melanoma, and breast cancer mouse models, we show that CD73-expressing M-MDSCs in the tumor microenvironment (TME) exhibit superior cell...
Our earlier work has shown that a unique stem cell-based vaccine comprises of murine embryonic cells (ESCs) and fibroblasts expressing the immunostimulant granulocyte-macrophage colony stimulating factor (GM-CSF) successfully protects mice from outgrowth an implantable form lung cancer. The use live ESCs raises potential risks inducing teratomas autoimmunity. We have attempted to improve safety utility this prophylactic by employing exosomes derived engineered produce GM-CSF (ES-exo/GM-CSF...
The sentinel lymph node (SLN) is the first encountered by a metastatic cancer cell and serves as predictor of poor prognosis, patients with clinically occult SLN metastases are classified stage III elevated rates recurrence diminished overall survival. However, dynamics immune infiltrates in SLNs remain poorly characterized. Here, using an unbiased Cellular Indexing Transcriptomes Epitopes Sequencing (CITE-seq) technique, we profiled 97,777 cells from tissues obtained stages I/II cutaneous...
Abstract Purpose: Improving our understanding of the immunologic response to cancer cells within sentinel lymph nodes (SLN) primary tumors is expected identify new approaches stimulate clinically meaningful immunity. Experimental Design: We used mass cytometry by time-of-flight (CyTOF), flow cytometry, and T-cell receptor immunosequencing conduct simultaneous single-cell analyses immune in SLNs patients with melanoma. Results: found increased effector-memory αβ T cells, TCR clonality, γδ...
Background: On-demand dosing of preexposure prophylaxis (PrEP) requires accurate prediction sex; however, abilities among young men who have sex with (YMSM) not been characterized. Setting: A nationally recruited prospective cohort YMSM ages 16–24 years. Methods: We followed 120 for 8 weeks using digital daily surveys (DDSs) to measure engagement in and anal over 24 hours, along condom use other encounter-level circumstances. Our main outcome, an “unpredicted spontaneous encounter,” was...
Abstract This study quantitatively examined factors related to young men who have sex with (YMSM)'s decisions use pre-exposure prophylaxis (PrEP) by their history of PrEP and qualitatively elicited perspectives on options. Higher proportions YMSM had never used (vs. ever used) considered the following as important in PrEP: (a) Returning follow-up visits ( p = .02), (b) having talk about sex/PrEP providers .013), (c) people assuming they are infected HIV .021), (d) family finding out .001),...
Efficient intracellular delivery of biomolecules is required for a broad range biomedical research and cell-based therapeutic applications. Ultrasound-mediated sonoporation an emerging technique rapid biomolecules. Sonoporation occurs when cavitation gas-filled microbubbles forms transient pores in nearby cell membranes, which enables uptake from the surrounding fluid. Current techniques vitro cells suspension are limited by slow throughput, variability ultrasound exposure conditions each...
Abstract Although immune checkpoint inhibitor (ICI) therapy has provided robust results in many cancer types such as melanoma and lung cancer, a large percentage of patients remain unresponsive to this therapy. Emerging evidence strongly suggests that one the contributing factors ICI resistance is monocytic myeloid derived suppressor cells (M-MDSCs) accumulate late-stage patients. These M-MDSCs are subset innate possess potent immunosuppressive activity against T lymphocytes. Here we provide...
Cell-based immunotherapies, such as CAR-T therapy, have recently demonstrated significant efficacy for treatment of blood cancers and other diseases. However, current methods cell loading processing these therapies are time-consuming, expensive, inefficient, which limits patient access safety this potentially life-saving therapy. To address limitations, we developing a novel 3-D-printed acoustofluidic system integrates ultrasound (5 MHz) flow chamber to consistently induce molecular delivery...
Supplementary Figure from Single‐Cell Immune Mapping of Melanoma Sentinel Lymph Nodes Reveals an Actionable Immunotolerant Microenvironment
Supplementary Figure from Single‐Cell Immune Mapping of Melanoma Sentinel Lymph Nodes Reveals an Actionable Immunotolerant Microenvironment
Supplementary Data from Single‐Cell Immune Mapping of Melanoma Sentinel Lymph Nodes Reveals an Actionable Immunotolerant Microenvironment
Supplementary Figure from Single‐Cell Immune Mapping of Melanoma Sentinel Lymph Nodes Reveals an Actionable Immunotolerant Microenvironment
Supplementary Figure from Single‐Cell Immune Mapping of Melanoma Sentinel Lymph Nodes Reveals an Actionable Immunotolerant Microenvironment
Supplementary Figure from Single‐Cell Immune Mapping of Melanoma Sentinel Lymph Nodes Reveals an Actionable Immunotolerant Microenvironment
Supplementary Figure from Single‐Cell Immune Mapping of Melanoma Sentinel Lymph Nodes Reveals an Actionable Immunotolerant Microenvironment
Supplementary Data from Single‐Cell Immune Mapping of Melanoma Sentinel Lymph Nodes Reveals an Actionable Immunotolerant Microenvironment
Supplementary Figure from Single‐Cell Immune Mapping of Melanoma Sentinel Lymph Nodes Reveals an Actionable Immunotolerant Microenvironment
<div>AbstractPurpose:<p>Improving our understanding of the immunologic response to cancer cells within sentinel lymph nodes (SLN) primary tumors is expected identify new approaches stimulate clinically meaningful immunity.</p>Experimental Design:<p>We used mass cytometry by time-of-flight (CyTOF), flow cytometry, and T-cell receptor immunosequencing conduct simultaneous single-cell analyses immune in SLNs patients with melanoma.</p>Results:<p>We found...
<div>AbstractPurpose:<p>Improving our understanding of the immunologic response to cancer cells within sentinel lymph nodes (SLN) primary tumors is expected identify new approaches stimulate clinically meaningful immunity.</p>Experimental Design:<p>We used mass cytometry by time-of-flight (CyTOF), flow cytometry, and T-cell receptor immunosequencing conduct simultaneous single-cell analyses immune in SLNs patients with melanoma.</p>Results:<p>We found...
Supplementary Data from Single‐Cell Immune Mapping of Melanoma Sentinel Lymph Nodes Reveals an Actionable Immunotolerant Microenvironment
Supplementary Figure from Single‐Cell Immune Mapping of Melanoma Sentinel Lymph Nodes Reveals an Actionable Immunotolerant Microenvironment
Supplementary Figure from Single‐Cell Immune Mapping of Melanoma Sentinel Lymph Nodes Reveals an Actionable Immunotolerant Microenvironment