A5003671599- Alcohol Consumption and Health Effects
- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cancer-related molecular mechanisms research
- Analytical Chemistry and Chromatography
- Biochemical effects in animals
- Acupuncture Treatment Research Studies
- Healthcare and Venom Research
- Advanced Chemical Sensor Technologies
- Cancer, Lipids, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Lipid metabolism and disorders
- Traditional Chinese Medicine Studies
- Gut microbiota and health
- Drug-Induced Hepatotoxicity and Protection
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Extracellular vesicles in disease
- Tryptophan and brain disorders
- Fibroblast Growth Factor Research
- Immune Cell Function and Interaction
- Diet and metabolism studies
- RNA and protein synthesis mechanisms
University of Louisville
2018-2025
University of Louisville Hospital
2024
Zhujiang Hospital
2023
Southern Medical University
2023
Air Force Medical University
2010-2014
Anhui University of Traditional Chinese Medicine
2012-2014
Xi'an Jiaotong University
2014
Shanghai Traditional Chinese Medicine Hospital
2010
Shanxi University of Traditional Chinese Medicine
2010
Beijing University of Chinese Medicine
2009
Cholestatic liver disease is characterized by gut dysbiosis and excessive toxic hepatic bile acids (BAs). Modification of microbiota repression BA synthesis are potential strategies for the treatment cholestatic disease. The purpose this study was to examine effects understand mechanisms probiotic Lactobacillus rhamnosus GG (LGG) on synthesis, injury, fibrosis in duct ligation (BDL) multidrug resistance protein 2 knockout (Mdr2-/- ) mice.Global intestine-specific farnesoid X receptor (FXR)...
Plant exosomes protect plants against infection; however, whether edible plant can mammalian hosts infection is not known. In this study, we show that ginger exosome-like nanoparticles (GELNs) are selectively taken up by the periodontal pathogen Porphyromonas gingivalis in a GELN phosphatidic acid (PA) dependent manner via interactions with hemin-binding protein 35 (HBP35) on surface of P. gingivalis. Compared PA (34:2), (34:1) did interact HBP35, indicating degree unsaturation plays...
Macrophages have been linked to tumor initiation, progression, metastasis, and treatment resistance. However, the transcriptional regulation of macrophages driving protumor function remains elusive. Here, we demonstrate that transcription factor c-Maf is a critical controller for immunosuppressive macrophage polarization in cancer. controls many M2-related genes has direct binding sites within conserved noncoding sequence Csf-1r gene promotes M2-like macrophage–mediated T cell suppression...
Alcohol-associated liver disease (ALD) is a major cause of mortality. Gut barrier dysfunction-induced bacterial translocation and endotoxin release contribute to the pathogenesis ALD. Probiotic
Diet and bile play critical roles in shaping gut microbiota, but the molecular mechanism underlying interplay with intestinal microbiota is unclear. Here, we showed that lemon-derived exosome-like nanoparticles (LELNs) enhance lactobacilli toleration to bile. To decipher mechanism, used Lactobacillus rhamnosus GG (LGG) as proof of concept show LELNs LGG resistance via limiting production Msp1 Msp3, resulting decrease accessibility cell membrane. Furthermore, found decline Msps protein levels...
Clostridioides difficile (C. diff) is the leading cause of antibiotic-associated colitis. Here, we report that lemon exosome-like nanoparticles (LELNs) manipulated probiotics to inhibit C. diff infection (CDI). LELN-manipulated Lactobacillus rhamnosus GG (LGG) and Streptococcus thermophilus ST-21 (STH) (LELN-LS) decrease CDI mortality via an LELN-mediated increase in bile resistance gut survivability. LELN-LS treatment increases AhR ligands indole-3-lactic acid (I3LA) indole-3-carboxaldehyde...
We report a reverse phase chromatography mass spectrometry (LC-MS) method for simultaneous quantification of nucleosides and nucleotides from biological samples, where compound identification was achieved by tier-wise approach via external calibration. A total 65 authentic standards were used the platform development. The limit detection (LOD) those compounds ranged 0.05 nmol/L to 1.25 μmol/L, their (LOQ) 0.10 2.50 μmol/L. Using developed method, human plasma, urine, rat liver quantified....
BACKGROUND Myocardial ischemia reperfusion (I/R) injury is associated with complex pathophysiological changes characterized by pH imbalance, the accumulation of lipid peroxidation products acrolein and 4-hydroxy
Background and Aims: Intestinal farnesoid X receptor (FXR) plays a critical role in alcohol‐associated liver disease (ALD). We aimed to investigate whether alcohol‐induced dysbiosis increased intestinal microRNA194 (miR194) that suppressed Fxr transcription Lactobacillus rhamnosus GG–derived exosome‐like nanoparticles (LDNPs) protected against ALD through regulation of miR194‐FXR signaling mice. Approach Results: Binge‐on‐chronic alcohol exposure mouse model was utilized. In addition the...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is intricately related to the reprogramming of host metabolism. However, existing studies have mainly focused on peripheral blood samples and barely identified specific metabolites that are critically involved in pathology disease 2019 (COVID-19). In current small-scale study, we performed metabolic profiling plasma (n = 61) paired bronchoalveolar lavage fluid (BALF) 20) using parallel two-dimensional liquid...
Alterations in gut bacterial homeostasis result changes intestinal metabolites. To investigate the effects of alcohol on fecal metabolites and role cathelicidin-related antimicrobial peptide (CRAMP) alcoholic liver disease (ALD), CRAMP knockout (KO) their control wild type (WT) mice were fed a Lieber-DeCarli liquid diet with or without alcohol. Polar mouse feces analyzed by GC × GC-MS 2DLC-MS, concentrations short chain fatty acids (SCFAs) measured GC-MS. A total 95 190 detected...
Exposure to a single dose of polychlorinated biphenyls (PCBs) and 12-week high-fat diet (HFD) results in nonalcoholic steatohepatitis (NASH) mice by altering intracellular signaling inhibiting epidermal growth factor receptor signaling. Post-transcriptional chemical modification (PTM) RNA regulates biological processes, but the contribution epitranscriptomics PCB-induced steatosis remains unknown. This study tested hypothesis that PCB HFD exposure alters global epitranscriptome male mouse...
Cellular metabolism has been proposed to govern distinct γδ T cell effector functions, but the underlying molecular mechanisms remain unclear. We show that interleukin-17 (IL-17)-producing (γδT17) and interferon-γ (IFN-γ)-producing (γδT1) cells have differential metabolic requirements rate-limiting enzyme isocitrate dehydrogenase 2 (IDH2) acts as a checkpoint for their functions. Intriguingly, transcription factor c-Maf regulates γδT17 function through direct regulation of IDH2 promoter...
This study aims to assess the targeted effect and antitumor efficacy of Gambogic-acid-loaded particles (GA-Ps). GA-Ps with uniform particle sizes 69.8 ± 17.8 nm (GA-P1), 185.6 33.8 (GA-P2), 357.8 81.5 (GA-P3), 7.56 0.95 μm (GA-P4) were prepared using an electrospray technique exhibited extremely high entrapment efficiency. As size increased from nano- microscale, in vitro GA release rate sharply decreased. After tail-vein injection mice, GA-P samples GA-P1, GA-P2, GA-P3, GA-P4 improved...
Stable isotope-resolved metabolomics (SIRM) provides information regarding the relative activity of numerous metabolic pathways and contribution nutrients to specific metabolite pools; however, SIRM experiments can be difficult execute, data interpretation is challenging. Furthermore, standardization analytical procedures workflows remain significant obstacles for widespread reproducibility. Here, we demonstrate workflow a typical experiment suggest experimental controls measures...
The diverse characteristics and large number of entities make metabolite separation challenging in metabolomics. To date, there is not a singular instrument capable analyzing all types metabolites. In order to achieve better for higher peak capacity accurate identification quantification, we integrated GC × GC-MS parallel 2DLC-MS analysis polar test the performance developed system, 13 rats were fed different diets form two animal groups. Polar metabolites extracted from rat livers analyzed...
Our study using both untargeted and targeted metabolomics reveals the caffeine metabolic pathway is dysregulated in ALD. Three metabolites, 1-methylxanthine, paraxanthine, 5-acetylamino-6-amino-3-methyluracil, can differentiate severity of early-stage
Bile acids (BAs) play important functions in the development of alcohol‐associated liver disease (ALD). In current study, urine BA concentrations 38 patients with well‐described hepatitis (AH) as characterized by Model for End‐Stage Liver Disease (MELD), 8 alcohol‐use disorder (AUD), and 19 healthy controls (HCs) were analyzed using liquid chromatography–mass spectrometry. Forty‐three BAs identified, 22 had significant changes their abundance levels AH. The potential associations clinical...
Dysregulated metabolism is a hallmark of cancer cells and driven in part by specific genetic alterations various oncogenes or tumor suppressors. The retinoblastoma protein (pRb) suppressor that canonically regulates cell cycle progression; however, recent studies have highlighted functional role for pRb controlling cellular metabolism. Here, we report loss the gene encoding (Rb1) transgenic mutant Kras-driven model lung results metabolic reprogramming. Our tracer using bolus dosing...
Introduction: Immune cell infiltration and metabolic reprogramming may have great impact on the tumorigenesis progression of malignancies. The interaction between these two factors in cervical cancer remains to be clarified. Here we constructed a gene set containing immune metabolism related genes applied this molecular subtyping cancer. Methods: Bulk sequencing single-cell data were downloaded from Cancer Genome Atlas (TCGA) database Gene Expression Omnibus (GEO) respectively. collected...