A5016196215- Extracellular vesicles in disease
- Gut microbiota and health
- MicroRNA in disease regulation
- Probiotics and Fermented Foods
- Reproductive Biology and Fertility
- IL-33, ST2, and ILC Pathways
- Cholesterol and Lipid Metabolism
- Antimicrobial Peptides and Activities
- Ovarian function and disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- COVID-19 Clinical Research Studies
- RNA regulation and disease
- Immune cells in cancer
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- S100 Proteins and Annexins
- Adipokines, Inflammation, and Metabolic Diseases
- Inflammasome and immune disorders
- Intracerebral and Subarachnoid Hemorrhage Research
- Amoebic Infections and Treatments
- Helicobacter pylori-related gastroenterology studies
- Phagocytosis and Immune Regulation
- Escherichia coli research studies
- Acute Kidney Injury Research
- Streptococcal Infections and Treatments
- Ginger and Zingiberaceae research
University of Louisville
2018-2025
University of Louisville Hospital
2024-2025
James Graham Brown Foundation
2021
All India Institute of Medical Sciences
2011-2021
Highlights•Plant exosome-like nanoparticles (ELNs) are taken up by gut bacteria•The lipid composition of ELNs determines uptake specific bacteria•ELN RNAs affect bacterial genes, notably affecting Lactobacillus production I3A•ELN-mediated I3A alterations IL-22 production, resulting in ameliorated colitisSummaryThe microbiota can be altered dietary interventions to prevent and treat various diseases. However, the mechanisms which food products modulate commensals remain largely unknown. We...
Abstract High-fat diet (HFD) decreases insulin sensitivity. How high-fat causes resistance is largely unknown. Here, we show that lean mice become resistant after being administered exosomes isolated from the feces of obese fed a HFD or patients with type II diabetes. altered lipid composition predominantly phosphatidylethanolamine (PE) in animals (L-Exo) to phosphatidylcholine (PC) (H-Exo). Mechanistically, intestinal H-Exo taken up by macrophages and hepatocytes, leading inhibition...
Lung inflammation is a hallmark of coronavirus disease 2019 (COVID-19). In this study, we show that mice develop inflamed lung tissue after being administered exosomes released from the epithelial cells exposed to severe acute respiratory syndrome 2 (SARS-CoV-2) Nsp12 and Nsp13 (exosomesNsp12Nsp13). Mechanistically, exosomesNsp12Nsp13 are taken up by macrophages, leading activation nuclear factor κB (NF-κB) subsequent induction an array inflammatory cytokines. Induction tumor necrosis...
Plant exosomes protect plants against infection; however, whether edible plant can mammalian hosts infection is not known. In this study, we show that ginger exosome-like nanoparticles (GELNs) are selectively taken up by the periodontal pathogen Porphyromonas gingivalis in a GELN phosphatidic acid (PA) dependent manner via interactions with hemin-binding protein 35 (HBP35) on surface of P. gingivalis. Compared PA (34:2), (34:1) did interact HBP35, indicating degree unsaturation plays...
Background: Obesity is becoming a global epidemic and reversing the pathological processes underlying obesity metabolic co-morbidities challenging.Obesity induced chronic inflammation including brain hallmark of via gut-brain axis.The objective this study was to develop garlic exosome-like nanoparticles (GaELNs) that inhibit systemic as well inflammatory activity reverse HFD in mice.Methods: GELNs were isolated administrated orally into fed mice.GaELNs fluorescent labeled for monitoring...
Gut microbiota function has numerous effects on humans and the diet consume emerged as a pivotal determinant of gut function. Here, new concept that can be trained by diet-derived exosome-like nanoparticles (ELNs) to release healthy outer membrane vesicles (OMVs) is introduced. Specifically, OMVs released from garlic ELN (GaELNs) human Akkermansia muciniphila (A. muciniphila) reverse high-fat diet-induced type 2 diabetes (T2DM) in mice. Oral administration GaELNs A. traffick brain where they...
Abstract Diet has emerged as a key impact factor for gut microbiota function. However, the complexity of dietary components makes it difficult to predict specific outcomes. Here we investigate plant-derived nanoparticles (PNP) on and metabolites in context cancer immunotherapy with humanized gnotobiotic mouse model. Specifically, show that ginger-derived exosome-like nanoparticle (GELN) preferentially taken up by Lachnospiraceae Lactobacillaceae mediated digalactosyldiacylglycerol (DGDG)...
Diet manipulation is the basis for prevention of obesity and diabetes. The molecular mechanisms that mediate diet-based insulin resistance are not well understood. Here, as proof-of-concept, ginger-derived nanoparticles (GDNP) were used studying underlying GDNP mediated high-fat diet induced resistance.
Diet and bile play critical roles in shaping gut microbiota, but the molecular mechanism underlying interplay with intestinal microbiota is unclear. Here, we showed that lemon-derived exosome-like nanoparticles (LELNs) enhance lactobacilli toleration to bile. To decipher mechanism, used Lactobacillus rhamnosus GG (LGG) as proof of concept show LELNs LGG resistance via limiting production Msp1 Msp3, resulting decrease accessibility cell membrane. Furthermore, found decline Msps protein levels...
Rationale:The obesity epidemic has expanded globally, due in large part to the increased consumption of high-fat diets (HFD), and risk major chronic diseases, including type 2 diabetes.Diet manipulation is foundation prevention treatment diabetes.The molecular mechanisms that mediate diet-based insulin resistance, however, remain be identified.Here, we report with orally administered ginger-derived nanoparticles (GDNP) prevents resistance by restoring homeostasis gut epithelial Foxa2...
Clostridioides difficile (C. diff) is the leading cause of antibiotic-associated colitis. Here, we report that lemon exosome-like nanoparticles (LELNs) manipulated probiotics to inhibit C. diff infection (CDI). LELN-manipulated Lactobacillus rhamnosus GG (LGG) and Streptococcus thermophilus ST-21 (STH) (LELN-LS) decrease CDI mortality via an LELN-mediated increase in bile resistance gut survivability. LELN-LS treatment increases AhR ligands indole-3-lactic acid (I3LA) indole-3-carboxaldehyde...
Microglia modulate pro-inflammatory and neurotoxic activities. Edible plant-derived factors improve brain function. Current knowledge of the molecular interactions between edible microglial cell is limited. Here an alcohol-induced chronic inflammation model used to identify that novel target oat nanoparticles (oatN). Oral administration oatN inhibits improves memory function mice are fed alcohol. Mechanistically, ethanol activates dectin-1 mediated inflammatory pathway. OatN taken up by...
Tumor-specific delivery of therapeutics is challenging.One the major hurdles for successfully delivering targeted agents by nanovectors filtering role liver in rapidly sequestering from circulation.Exosomes, a type endogenous nanoparticle, circulate continuously peripheral blood and play intercellular communication.The aim this study was to determine whether level exosomes has an effect on nanovector efficiency agents.Methods: Exosomes were isolated intravenously (I.V.) injected into...
Endogenous pathogens can constrain virulence to ensure survival in the host. Pathogenic state be controlled by metabolic responses prevailing microenvironment; however, coupling and effector mechanisms are not well understood. Flux through One Carbon Metabolism (OCM) pathway modulate of oral pathobiont Porphyromonas gingivalis , here we show that this is tyrosine phosphorylation-dependent differential partitioning gingipain proteases. The OCM essential precursor pABA inhibits low molecular...
Interleukin-33 (IL-33) acts as an ‘alarmin’, and its role has been demonstrated in driving immune regulation inflammation many human diseases. However, the precise mechanism of action IL-33 regulating neutrophil macrophage functioning is not defined advanced atherosclerosis (aAT) patients. Further, extracellular trap (NET) formation aAT consequent effect on function known. In present study, we recruited n = 52 patients control subjects. The neutrophils were isolated from both groups via...
Abstract Background Ample amount of data suggests role rapid eye movement (REM) sleep deprivation as the cause and effect mania. Studies have also suggested disrupted circadian rhythms contributing to pathophysiology mood disorders, including bipolar disorder. However, studies pertaining genes lithium treatment on clock are scant. Thus, we wanted determine effects REM expression core whether epigenetics is involved. Next, explore ultrastructural abnormalities in hippocampus. Moreover, were...
Abstract Extracellular vesicles (EVs) contain more than 100 proteins. Whether there are EVs proteins that act as an ‘organiser’ of protein networks to generate a new or different biological effect from identified in EV‐producing cells has never been demonstrated. Here, proof‐of‐concept, we demonstrate EV‐G12D‐mutant KRAS serves leader forms complex and promotes lung inflammation tumour growth via the Fn1/IL‐17A/FGF21 axis. Mechanistically, contrast cytosol derived G12D‐mutant EVs‐producing...
(iScience 21, 308–327; November 22, 2019) In the originally published paper, author Jun Yan’s name was written incorrectly as “Yan Jun.” This has now been corrected online. The authors apologize for this oversight. Plant-Derived Exosomal Nanoparticles Inhibit Pathogenicity of Porphyromonas gingivalisSundaram et al.iScienceOctober 2019In BriefBacteriology; Biochemistry; Biological Sciences; Immunology; Microbiology; Molecular Biology; Oral Microbiology Full-Text PDF Open Access
There is scanty data in India on polycystic ovary syndrome (PCOS) from several small, undersized, convenience-based studies employing differing diagnostic criteria and reporting varied regional prevalence. It difficult to draw clear-cut conclusions these studies; therefore, the present multicentric, well-designed, large-scale representative countrywide epidemiological study PCOS across was conceived with aim generate actual prevalence rates of a total sample size approximately 9000 individuals.
Severe colitis is a common side effect of chemotherapy in cancer patients. In this study, we attempted to enhance the viability probiotics gastric acid environment and improve induced by dextran sulfate sodium (DSS) docetaxel.We purified Lactobacillus from yogurt estimated their growth at pH 6.8 2.0. further investigation, bacterial biofilm formation was used define mechanism which administration rhamnosus (LGG) via oral gavage alleviates intestine permeability mice DSS docetaxel. The...
Recent therapeutic approaches have improved survival rate for women with breast cancer, but the metastatic cancer is still low. Exosomes released by various cells are involved in all steps of development.We established multimodal imaging report expression lentivirus vectors pGluc and pBirA to investigate secreted exosomes. Comparative microRNA (miRNA) analysis was performed miRNA qPCR array mice lung metastasis. The co-immunoprecipitation chromatin immunoprecipitation assays were used...