Abstract Epidemiological evidence indicates that phytoestrogens inhibit cancer formation and growth, reduce cholesterol levels, show benefits in treating osteoporosis. At least some of these activities are mediated through the interaction with estrogen receptors α β (ERα ERβ). Resveratrol, trans-3,5,4′-trihydroxystilbene, is a phytoestrogen grapes present red wine. Resveratrol was shown to bind ER cytosolic extracts from MCF-7 rat uteri. However, contribution ERα vs. ERβ this binding...

Select changes in microRNA (miRNA) expression correlate with estrogen receptor α (ERα) breast tumors. miR-21 is higher ERα positive than negative tumors, but no one has examined how estradiol (E 2 ) regulates cancer cells. Here we report that E inhibits MCF-7 human The -induced reduction was inhibited by 4-hydroxytamoxifen (4-OHT), ICI 182 780 (Faslodex), and siRNA indicating the suppression ERα-mediated. ERβ agonists PPT DPN 4-OHT increased expression. luciferase activity from reporters...

Estrogen has direct and indirect effects on mitochondrial activity, but the mechanisms mediating these remain unclear. Others reported that long-term estradiol (E(2)) treatment increased nuclear respiratory factor-1 (NRF-1) protein in cerebral blood vessels of ovariectomized rats. NRF-1 is a transcription factor regulates expression nuclear-encoded genes, e.g. A (TFAM), control genome. Here we tested hypothesis E(2) increases resulting coordinate increase nuclear- mitochondrial- encoded...

The estrogen receptor (ER) is a transcription factor that binds to specific DNA sequence found in the regulatory regions of estrogen-responsive genes, called response element (ERE). Many genes contain EREs have been identified, and most these one or more changes from core consensus sequence, 13-nucleotide segment with 10 nucleotides forming an inverted repeat. A number multiple copies imperfect EREs. In order understand why natural developed this manner, we attempted define basic...

One mechanism by which ligand-activated estrogen receptors α and β (ERα ERβ) stimulate gene transcription is through direct ER interaction with specific DNA sequences, response elements (EREs). ERE-bound recruits coactivators that transcription. Binding of to natural synthetic EREs different nucleotide sequences alters binding affinity, conformation, transcriptional activity, indicating the ERE sequence an allosteric effector action. Here we tested hypothesis alterations in conformation...

Epidemiological studies correlate moderate red wine consumption to reduced incidence of cardiovascular disease. Resveratrol is a polyphenolic compound in that has cardioprotective effects rodents. Although endothelial cell (EC) indicate micromolar resveratrol diverse biological activities, these concentrations are not physiologically relevant because human oral ingestion provides only brief exposure nanomolar plasma levels. Previously, we reported activated ERK1/2 signaling bovine aortic ECs...

Women outlive men, but life expectancy is not influenced by hormone replacement (estrogen + progestin) therapy. Estrogens appear to protect brain, cardiovascular tissues, and bone from aging. regulate genes directly through binding estrogen receptors alpha beta (ERα ERβ) that are ligand-activated transcription factors indirectly activating plasma membrane-associated ER which, in turns, activates intracellular signaling cascades leading altered gene expression. MicroRNAs (miRNAs) short (19-25...

The purpose of this article is to provide a conceptual framework for mentoring as an added component learning organization in the context adult and development theories. Mentoring traditionally process which experienced person (the mentor) guides another mentee or protégé) her his own ideas, learning, personal/professional competence. uses transformational theory through critical reflection non-judgmental manner addresses andragogical principle that experience richest source learning. In...

Abstract MicroRNAs are dysregulated in breast cancer. Heterogeneous Nuclear Ribonucleoprotein A2/B1 (HNRNPA2/B1) is a reader of the N(6)-methyladenosine (m6A) mark primary-miRNAs (pri-miRNAs) and promotes DROSHA processing to precursor-miRNAs (pre-miRNAs). We examined expression writers, readers, erasers m6A report that HNRNPA2/B1 higher tamoxifen-resistant LCC9 cancer cells as compared parental, tamoxifen-sensitive MCF-7 cells. To examine how increased affects miRNA expression, was...

The role of miRNAs in acquired endocrine-resistant breast cancer is not fully understood. One hallmark tumor progression epithelial-to-mesenchymal transition (EMT), characterized by a loss cell adhesion resulting from reduced E-cadherin and increased mobility. miR-200 family members regulate EMT suppressing expression transcriptional repressors ZEB1/2. Previously we reported that the miR-200a, miR-200b, miR-200c was lower LY2 endocrine-resistant, mesenchymal cells compared to parental,...

G-protein-coupled estrogen receptor (GPER/GPR30) was reported to bind 17β-estradiol (E2), tamoxifen, and ICI 182,780 (fulvestrant) promotes activation of epidermal growth factor (EGFR)-mediated signaling in breast, endometrial thyroid cancer cells. Although lung adenocarcinomas express receptors α β (ERα ERβ), the expression GPER has not been investigated. The purpose this study examine cancer. patterns various lines tumors were investigated using standard quantitative real time PCR (at mRNA...