A5002140834- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Monoclonal and Polyclonal Antibodies Research
- Renal Transplantation Outcomes and Treatments
- Blood groups and transfusion
- Blood donation and transfusion practices
- RNA modifications and cancer
- Hepatitis C virus research
- Cancer Genomics and Diagnostics
- Mitochondrial Function and Pathology
- Vaccine Coverage and Hesitancy
- Cancer, Lipids, and Metabolism
- Polyomavirus and related diseases
- Transplantation: Methods and Outcomes
- Chronic Lymphocytic Leukemia Research
- Ginkgo biloba and Cashew Applications
- Clinical Laboratory Practices and Quality Control
- Adipose Tissue and Metabolism
- Prenatal Screening and Diagnostics
- Nuclear Receptors and Signaling
- Myasthenia Gravis and Thymoma
- Single-cell and spatial transcriptomics
- Extracellular vesicles in disease
- Full-Duplex Wireless Communications
University of Minnesota
2023-2025
University of Minnesota Medical Center
2023-2025
Northwestern University
2021-2022
University of Louisville
2013-2020
The role of miRNAs in acquired endocrine-resistant breast cancer is not fully understood. One hallmark tumor progression epithelial-to-mesenchymal transition (EMT), characterized by a loss cell adhesion resulting from reduced E-cadherin and increased mobility. miR-200 family members regulate EMT suppressing expression transcriptional repressors ZEB1/2. Previously we reported that the miR-200a, miR-200b, miR-200c was lower LY2 endocrine-resistant, mesenchymal cells compared to parental,...
Abstract Anacardic acid (AnAc), a potential dietary agent for preventing and treating breast cancer, inhibited the proliferation of estrogen receptor α (ERα) positive MCF-7 MDA-MB-231 triple negative cancer cells. To characterize regulators AnAc action, cells were treated 6 h with purified 24:1n5 congener followed by next generation transcriptomic sequencing (RNA-seq) network analysis. We reported that AnAc-differentially regulated miRNA transcriptomes in each cell line now identify...
Following transplantation, it is recommended that human leukocyte antigen (HLA) donor specific antibody (DSA) monitoring for allograft surveillance be tailored to the patient's antibody-mediated rejection (AMR) risk and immunosuppression needs. However, at our institution, DSA orders were placed more frequently than recommended, with daily duplications due inconsistent ordering across departments (outpatient, emergency, inpatient). We evaluated effectiveness of a non-interruptive clinical...
Aberrant microRNA expression contributes to breast cancer progression and endocrine resistance. We reported that although tamoxifen stimulated miR-29b-1/a transcription in (TAM)-resistant cells, ectopic of did not drive TAM-resistance MCF-7 cells. However, overexpression significantly repressed TAM-resistant LCC9 cell proliferation, suggesting is mediating TAM resistance but acts as a tumor suppressor The target genes this activity were unknown. Here, we identify miR-29b-1 miR-29a...
Despite improvements in the treatment of endocrine-resistant metastatic disease using combination therapies patients with estrogen receptor α (ERα) primary tumors, mechanisms underlying endocrine resistance remain to be elucidated. Non-coding RNAs (ncRNAs), including microRNAs (miRNA) and long non-coding (lncRNA), are targets regulators cell signaling pathways their exosomal transport may contribute metastasis. Previous studies have shown that a low expression miR-29a-3p miR-29b-3p is...
MicroRNAs are biomarkers and potential therapeutic targets for breast cancer. Anacardic acid (AnAc) is a dietary phenolic lipid that inhibits both MCF-7 estrogen receptor α (ERα) positive MDA-MB-231 triple negative cancer (TNBC) cell proliferation with IC50s of 13.5 35 μM, respectively. To identify mediators AnAc action in cancer, we profiled the genome-wide microRNA transcriptome (microRNAome) these two lines altered by 24:1n5 congener. Whole genome expression profiling (RNA-seq) subsequent...
Reduction of immunosuppression (IS) upon detection Polyomavirus (BK) viremia is widely used to prevent BK virus nephropathy. This retrospective case-control study assesses the frequency de novo donor-specific antibodies (dnDSA) in renal transplant recipients with IS modulation due and associated risk antibody mediated rejection.Our cohort included kidney transplantation between 2007 2017 clinical, HLA antibody, biopsy data. positivity was defined as >10 000 c/ml or proven A total 190 cases...
κ and λ free light chains (FLCs) are monitored to aid in the diagnosis of plasma cell disorders. Our goal was validate Diazyme Human assays on Beckman Coulter UniCel DxC 800 Synchron compare Freelite Roche Cobas Integra. Linearity verification, within- between-run precision, method comparison, reference range (RR) verification were conducted using CLSI guidelines. Statistical analysis performed EP Evaluator®. Mean, SD, CV, bias determined. FLC assay linear within 0.00–191.00 mg/L....
Abstract Background Donor-derived cell-free DNA (dd-cf-DNA) tests offer a non-invasive alternative to biopsy for allograft surveillance in solid organ transplantation. Unlike biopsies, dd-cf-DNA are not subject interpretation bias and have the potential detect subclinical rejection, which can lead graft dysfunction poor outcomes. At our institution, testing is currently offered as send out test we sought evaluate its efficacy monitoring post-transplant patients. Methods We retrospectively...
For many laboratories, autoimmune encephalopathy (AE) panels are send-out tests. These tests expensive, and ordering patterns vary greatly. There is also a lack of consensus on which panel to order poor understanding the clinical utility these panels. challenges were presented our newly formed, multidisciplinary, diagnostic stewardship committee (DSC). Through this collaboration, we developed an algorithm for AE panels; combining criteria with practice guidelines.We analyzed test-ordering in...
Abstract Background Lithium is prescribed clinically to manage mania in patients with bipolar disorder. However, blood levels higher than 1.5 mmol/L (12 h post administration) indicate toxicity accompanied by severe nausea and vomiting, hand tremors, confusion, vision changes. If neglected, lithium can lead coma, brain damage, or even death. Diligent monitoring of periodically critical preventing toxicity. Case Presentation: A 37 y/o female schizophrenia disorder on since 2019 visited the...
Abstract Acquired endocrine-resistance occurs in ~ 40% of estrogen receptor α (ERα)+ breast cancer patients after tamoxifen (TAM) or aromatase inhibitor therapy. MicroRNAs (miRs) are dysregulated cancer, but their contribution to is not yet completely understood. Microarray analysis miRNAs TAM-sensitive MCF-7 versus TAM-resistant LY2 cells (derived from cells) identified differential regulation miR-29b-1 and miR-29a. miR-29a miR-29b-1, which co-transcribed as a single primary-miR, were...
Abstract As part of our laboratory testing utilization at University Louisville Hospital (ULH), any send-out test ≥$500 must be assessed by the clinical chemistry fellow or pathology resident for necessity and as a way to provide consultation ordering physician on appropriate utilization. In 2018, we noticed prominent increase in volume panels autoimmune encephalopathy (AE) related disorders being ordered. This was met with considerable cost hospital, lack consensus which should ordered,...