A5031126219- Bacterial Identification and Susceptibility Testing
- Bacillus and Francisella bacterial research
- Antibiotic Resistance in Bacteria
- Streptococcal Infections and Treatments
- Infective Endocarditis Diagnosis and Management
- Antibiotics Pharmacokinetics and Efficacy
- Diabetes Management and Research
- Antimicrobial Resistance in Staphylococcus
- Clinical Laboratory Practices and Quality Control
- Antibiotic Use and Resistance
- Bacteriophages and microbial interactions
- Meta-analysis and systematic reviews
- Monoclonal and Polyclonal Antibodies Research
- Clostridium difficile and Clostridium perfringens research
- Zoonotic diseases and public health
- Chemical Safety and Risk Management
- Autoimmune and Inflammatory Disorders Research
- Ocular Infections and Treatments
- Sepsis Diagnosis and Treatment
- Yersinia bacterium, plague, ectoparasites research
- Systemic Lupus Erythematosus Research
- Diabetes Management and Education
- Inflammasome and immune disorders
- Nosocomial Infections in ICU
- Immune Cell Function and Interaction
University of Louisville Hospital
2001-2024
Children's Hospital of Philadelphia
2014-2024
University of Louisville
2013-2024
Siemens Healthcare (United States)
2023
Genomics (United Kingdom)
2020
Novo Nordisk (United States)
2018-2019
Rochester Institute of Technology
2013
HealthInsight
2003
VA Southern Nevada Healthcare System
2001
United States Army Reserve
1993
The critical role of the microbiology laboratory in infectious disease diagnosis calls for a close, positive working relationship between physician and microbiologists who provide enormous value to health care team. This document, developed by both clinical experts, provides information on which tests are valuable contexts, that add little or no diagnostic decisions. Sections divided into anatomic systems, including Bloodstream Infections Cardiovascular System, Central Nervous System...
The critical nature of the microbiology laboratory in infectious disease diagnosis calls for a close, positive working relationship between physician/advanced practice provider and microbiologists who provide enormous value to healthcare team. This document, developed by experts adult pediatric clinical medicine, provides information on which tests are valuable contexts, that add little or no diagnostic decisions. document presents system-based approach rather than specimen-based approach,...
The critical nature of the microbiology laboratory in infectious disease diagnosis calls for a close, positive working relationship between physician/advanced practice provider and microbiologists who provide enormous value to healthcare team. This document, developed by experts adult pediatric clinical medicine, provides information on which tests are valuable contexts, that add little or no diagnostic decisions. document presents system-based approach rather than specimen-based approach,...
Abstract The critical nature of the microbiology laboratory in infectious disease diagnosis calls for a close, positive working relationship between physician and microbiologists who provide enormous value to healthcare team. This document, developed by experts both adult pediatric clinical medicine, provides information on which tests are valuable contexts, that add little or no diagnostic decisions. Sections divided into anatomic systems, including Bloodstream Infections Cardiovascular...
OBJECTIVE Latent autoimmune diabetes in adults (LADA) shares clinical features with both type 1 and 2 diabetes; however, there is ongoing debate regarding the precise definition of LADA. Understanding its genetic basis one potential strategy to gain insight into appropriate classification this subtype. RESEARCH DESIGN AND METHODS We performed first genome-wide association study LADA case subjects European ancestry versus population control (n = 2,634 vs. 5,947) compared against 2,454 968)...
Abstract The critical role of the microbiology laboratory in infectious disease diagnosis calls for a close, positive working relationship between physician and microbiologists who provide enormous value to health care team. This document, developed by both clinical experts, provides information on which tests are valuable contexts, that add little or no diagnostic decisions. Sections divided into anatomic systems, including Bloodstream Infections Cardiovascular System, Central Nervous...
Abstract Autoimmune diseases (AIDs) are polygenic affecting 7–10% of the population in Western Hemisphere with few effective therapies. Here, we quantify heritability paediatric AIDs (pAIDs), including JIA, SLE, CEL, T1D, UC, CD, PS, SPA and CVID, attributable to common genomic variations (SNP -h 2 ). SNP- h estimates most significant for T1D (0.863±s.e. 0.07) JIA (0.727±s.e. 0.037), more modest UC (0.386±s.e. 0.04) CD (0.454±0.025), largely consistent generally greater than that previously...
OBJECTIVE—To determine whether a comprehensive diabetes management program that included risk stratification and social marketing would improve clinical outcomes patient satisfaction within managed care organization (MCO). RESEARCH DESIGN AND METHODS—The 12-month prospective trial was conducted at primary clinics MCO involved 370 adults with diabetes. Measurements 1) the frequency of dilated eye foot examinations, microalbuminuria assessment, blood pressure measurement, lipid profile, HbA1c...
The Phoenix automated microbiology system (BD Diagnostics, Sparks, MD) is designed for the rapid identification (ID) and antimicrobial susceptibility testing (AST) of clinically significant human bacterial pathogens. We evaluated performance instrument in comparison with that MicroScan WalkAway (Dade Behring, West Sacramento, CA) ID AST gram-negative clinical strains challenge isolates Enterobacteriaceae (n = 150) nonfermentative bacilli (NFGNB; 45 8 isolates). discrepancies were resolved...
This study compared the activity of cefepime + zidebactam (FEP-ZID) and selected currently available antibacterial agents against a panel multidrug-resistant (MDR) clinical isolates chosen to provide an extreme challenge for activity. FEP–ZID had very broad potent in vitro spectrum activity, was highly active many MDR Enterobacterales, Pseudomonas aeruginosa, Acinetobacter baumannii. Notably, it inhibited producing carbapenemases Ambler classes A, B, D, P. aeruginosa with multiple resistance...
ABSTRACT The accurate detection of carbapenemase-producing organisms is a major challenge for clinical laboratories. Carba NP test highly but inconvenient, as it requires frequent preparation fresh imipenem solution. current study was designed to compare the two alternative tests accuracy and convenience. These were modified that utilized intravenous (i.v.) imipenem-cilastatin, which less expensive than reference standard powder, an updated version Rosco Neo-Rapid Carb kit, does not require...
Blood cultures (BCs) frequently become contaminated during the pre-analytic phase of collection leading to downstream ramifications. We present a summary performance improvement (PI) interventions provided by four hospital systems and common factors that contributed decreased blood culture contamination (BCC) rates. Each independently formed multidisciplinary team action plan for implementation their intervention, focusing on use educational training tools. Their goal was significantly...
ABSTRACT Fifty-nine Staphylococcus aureus isolates from one hundred blood cultures containing gram-positive cocci in clusters were identified by conventional methods and the BD GeneOhm StaphSR assay (SR). The SR misidentified three methicillin (meticillin)-resistant S. (MRSA) as methicillin-susceptible (MSSA), while MSSA isolate tested negative for . MRSA strains with MREJ types that cannot be detected currently available SR.
The majority of outer membrane (OM) lipoproteins in Gram-negative bacteria are tethered to the via an attached lipid moiety and oriented facing toward periplasmic space; a few have been shown be surface exposed. lipoprotein P6 from pathogenic bacterium nontypeable Haemophilus influenzae (NTHi) is exposed leading vaccine candidate for prevention NTHi infections. However, we recently found that not transmembrane protein as previously thought (L. V. Michel, B. Kalmeta, M. McCreary, J. Snyder,...
Peripheral blood mononuclear cells (PBMCs) are widely used as a model in the study of different human diseases. There is often time delay from collection to PBMC isolation during sampling process, which can result an experimental bias, particularly when performing single cell RNA-seq (scRNAseq) studies.This examined impact periods draw on subsequent transcriptome profiling types PBMCs by scRNAseq using 10X Chromium Single Cell Gene Expression assay.Examining five major constituting...
Juvenile idiopathic arthritis (JIA) is the most common chronic rheumatic disease among children, etiology of which involves a strong genetic component, but much underlying determinants still remain unknown. Our aim was to identify novel variants that predispose JIA. We performed genome-wide association study (GWAS) and replication in total 1166 JIA cases 9500 unrelated controls European ancestry. Correlation SNP genotype gene expression investigated. Then we conducted targeted resequencing...