A5040663465- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Genomics and Phylogenetic Studies
- Antibiotics Pharmacokinetics and Efficacy
- Vibrio bacteria research studies
- Animal Diversity and Health Studies
- Microbial Natural Products and Biosynthesis
- Probiotics and Fermented Foods
- Escherichia coli research studies
- Metabolomics and Mass Spectrometry Studies
- Antimicrobial Resistance in Staphylococcus
- Viral gastroenteritis research and epidemiology
- Hepatitis Viruses Studies and Epidemiology
- Bacterial biofilms and quorum sensing
- Pharmacological Effects of Natural Compounds
- Geochemistry and Elemental Analysis
- Dermatology and Skin Diseases
- Microbial Fuel Cells and Bioremediation
- Exercise and Physiological Responses
- Enzyme Production and Characterization
- Psidium guajava Extracts and Applications
- Cancer, Stress, Anesthesia, and Immune Response
- Salmonella and Campylobacter epidemiology
- Microbial bioremediation and biosurfactants
- Antibiotic Use and Resistance
Beni-Suef University
2013-2023
University of Louisville
2019-2022
Jewish Hospital
2022
Al-Azhar University
2020
Cairo University
2009
National Research Centre
1972
ABSTRACT A recent controversial hypothesis suggested that the bactericidal action of antibiotics is due to generation endogenous reactive oxygen species (ROS), a process requiring citric acid cycle (tricarboxylic [TCA] cycle). To test this hypothesis, we assessed ability oxacillin induce ROS production and cell death in Staphylococcus epidermidis strain 1457 an isogenic mutant. Our results confirm contributory role for TCA-dependent enhancing susceptibility S. toward β-lactam also revealed...
Essential oils are one of interesting natural products group that used in different aspects life due to their various biological activities. This study investigate the antimicrobial activities 9 herbal essential on survival and growth selected pathogenic spoilage microorganisms. were obtained by hydrodistillation method analyzed using GC/MS technique. The tested for activity against 2 Gram +ve, Staphylococcus aureus (S. aureus) Listeria innocua (L. innocua), −ve, Pseudomonas aeruginosa (P....
This study compared the activity of cefepime + zidebactam (FEP-ZID) and selected currently available antibacterial agents against a panel multidrug-resistant (MDR) clinical isolates chosen to provide an extreme challenge for activity. FEP–ZID had very broad potent in vitro spectrum activity, was highly active many MDR Enterobacterales, Pseudomonas aeruginosa, Acinetobacter baumannii. Notably, it inhibited producing carbapenemases Ambler classes A, B, D, P. aeruginosa with multiple resistance...
ABSTRACT The accurate detection of carbapenemase-producing organisms is a major challenge for clinical laboratories. Carba NP test highly but inconvenient, as it requires frequent preparation fresh imipenem solution. current study was designed to compare the two alternative tests accuracy and convenience. These were modified that utilized intravenous (i.v.) imipenem-cilastatin, which less expensive than reference standard powder, an updated version Rosco Neo-Rapid Carb kit, does not require...
Literature surveys, taxonomical differences, and bioassay results have been utilized in the discovery of new natural products to aid Actinomycetes isolate-selection. However, no or less investigation done on establishing differences metabolomic profiles isolated microorganisms. The study aims utilise bioassay- metabolomics-guided tools that included dereplication multivariate analysis NMR mass spectral data microbial extracts assist selection isolates for scaling-up production antimicrobial...
Abstract Background The rapid spread of antibiotic resistance has increased research interest in the discovery natural products, mainly from actinomycetes, which have been primary source antimicrobial compounds. This study aimed to isolate, characterize, and optimize production some bioactive compounds soil actinomycetes. Results One promising actinomycete, was molecularly identified as Streptomyces sp. designated MS. 10, showed broad-spectrum activity, including activity against...
Abstract Background Polycyclic aromatic hydrocarbons (PAHs) such as anthracene are one of the most toxic contaminants to our environment. Microbial biodegradation these xenobiotics is a cost-effective technological solution. The present study aimed recover some bacterial isolates from Beni-Suef Governorate in Egypt with high capabilities biodegradation. selected were molecularly characterized by 16S rRNA gene sequencing, degree was monitored using optical density (OD) and high-performance...
This study is designed to recover and characterize heavy metal resistant halophilic bacteria from Egypt in order identify mechanisms, plasmids' role, factors affecting lead (Pb) resistance, screen for potential industrial therapeutic enzyme production. Forty-five were recovered eleven locations Lake Qarun, Egypt. The WQL9 isolate showed high Pb resistance up 4 mM was other metals. 16S rRNA gene analysis phenotypic characteristics revealed that strain belongs the genus Halomonas. growth...
Abstract In a precarious world of rapidly growing pandemics, the field vaccine production has witnessed considerable growth. Bacillus Calmette-Guérin (BCG) is live-attenuated and part immunization program in 157 countries. The quality control based on potency test through viable cell enumeration. colony-forming unit (CFU) assay official method, however, it often yields fluctuating results, suffers from medium cracking, requires lengthy analysis (~ 28 days). Flow cytometric was proposed...
The current BD Kiestra™ total laboratory automation (TLA) system automates specimen inoculation, incubation, and digital visualization of cultures prior to initiation manual or semi-automated identification (ID) antimicrobial susceptibility testing (AST). study aimed compare the performance, in a clinical setting, fully automated research-use-only prototype, IdentifA/SusceptA (automated system), our TLA which utilizes IDs ASTs (current system).
Background The literature shows a growing emphasis on understanding the local patterns of antimicrobial resistance (AMR). We aimed to evaluate spectrum microorganisms that cause bloodstream infections (BSI) and their AMR in an Egyptian institution treating children with cancer. Methods conducted single-centre, retrospective, study confirmed primary, hospital-acquired, BSIs over one year. microbiological examination blood samples was done according Clinical Laboratory Standards Institute....
The purpose of this present study is to define the role cells innate immune system (i.e., NK cells) in infection and cancer investigate effect selected exercise program on natural killer cytotoxic post-mastectomy patients. Forty female patients have referred from an oncologist professor undergone mastectomies they oncology surgery department, physical therapy clinic National Cancer Institute (NCI), Cairo University. They ranged between 40 60 years. all were mastectomy. These randomly divided...
Chitosan and alginate salts are natural biopolymers that have gained recent attention in the biomedical sectors. Their properties allow them to become potential candidates as safe, cheap, effective vaccine adjuvants. The present study aimed enhance immunogenic response of a current injectable killed cholera (KCV) using chitosan salt adjuvants against alum. We tested KCV adjuvanted with alum, chitosan, sodium mice. Mice were immunized intraperitoneally or compared control unadjuvanted group....
Fifty samples of freshly spray-dried non-fat milk were taken from the Sakha plant. The quality this powdered was assessed by bacteriological, chemical, and physical tests. Bacterial counts showed following averages per gram; total viable counts, 13 × 106 at 30 C 6.8 37 C; thermoduric, 1.6 106; psychrophilic, 4.5 105; non-pathogenic staphylococci, 4 Group D streptococci, group I comprising Streptococcus faecium, durans bovis, 5.3 105 II faecalis its varieties zymogenes liquefaciens, 9.3 104;...
Carbapenemase-producing organisms (CPOs) are Gram-negative bacteria that typically resistant to most or all antibiotics and responsible for a global pandemic of high mortality. Rapid, accurate detection CPOs the classification their carbapenemases valuable tools reducing mortality CPO-associated infections, preventing spread CPOs, optimizing use new β-lactamase inhibitor combinations such as ceftazidime/avibactam, meropenem/vaborbactam imipenem/relebactam. The current study evaluated...
This study aimed to investigate the genetic basis for quinolones and fluoroquinolones insusceptibility in E.coli isolated from outpatients suspected with urinary tract infections (UTIs).Fifty-one nalidixic acid unsusceptible E. coli isolates were collected adult patients UTIs .Antimicrobial sensitivity testing was performed by disc diffusion test, minimum inhibitory concentrations of ciprofloxacin determined test strips.PCR amplification DNA sequencing carried out detection alterations...
The bacterial groups isolated at 30 C and 37 from spray-dried milk proved to be streptococci, micrococci, micro-bacteria, spore-formers in decreasing order. These bacteria were identified species.
Abstract Rapid, accurate detection of carbapenemase-producing organisms (CPOs) and the classification their carbapenemases are valuable tools for reducing mortality CPO-associated infections, preventing spread CPOs, optimizing use new β-lactamase inhibitor combinations such as ceftazidime/avibactam meropenem/vaborbactam. The current study evaluated performance CPO Complete, a novel, manual, phenotypic carbapenemase test. test was sensitivity specificity against 262 isolates...