A5072539940- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Hematopoietic Stem Cell Transplantation
- Renal Transplantation Outcomes and Treatments
- Diabetes and associated disorders
- Immunodeficiency and Autoimmune Disorders
- Transplantation: Methods and Outcomes
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Sarcoidosis and Beryllium Toxicity Research
- CAR-T cell therapy research
- Dermatology and Skin Diseases
- Blood groups and transfusion
- Cancer Genomics and Diagnostics
- Reproductive System and Pregnancy
- Urticaria and Related Conditions
- Acute Myeloid Leukemia Research
- Molecular Biology Techniques and Applications
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Pancreatic function and diabetes
- MicroRNA in disease regulation
- Immune Response and Inflammation
- Glycosylation and Glycoproteins Research
- Organ Transplantation Techniques and Outcomes
Children's Hospital of Philadelphia
2015-2024
University of Pennsylvania
2015-2024
California University of Pennsylvania
2021
Sebelas Maret University
2019
Michigan State University
2019
University of Cincinnati
2019
United States Agency for International Development
2019
National Institute for Occupational Safety and Health
2019
The Ohio State University
2018
Philadelphia University
2000-2017
We conducted a pilot clinical trial testing personalized vaccine generated by autologous dendritic cells (DCs) pulsed with oxidized whole-tumor cell lysate (OCDC), which was injected intranodally in platinum-treated, immunotherapy-naïve, recurrent ovarian cancer patients. OCDC administered alone (cohort 1, n = 5), combination bevacizumab 2, 10), or plus low-dose intravenous cyclophosphamide 3, 10) until disease progression exhaustion. A total of 392 doses were without serious adverse events....
Human T-cell-mediated autoimmune diseases are genetically linked to particular alleles of MHC class II genes. Susceptibility pemphigus vulgaris (PV), an disease the skin, is a rare subtype HLA-DR4 (DRB1*0402, 1 22 known DR4 subtypes). The PV-linked differs from rheumatoid arthritis-associated (DRB1*0404) only at three residues (DR beta 67, 70, and 71). caused by autoantibodies against desmoglein 3 (DG), T cells thought trigger autoantibody production this keratinocyte adhesion molecule....
Genome-wide association studies (GWAS) have been fruitful in identifying disease susceptibility loci for common and complex diseases. A remaining question is whether we can quantify individual risk based on genotype data, order to facilitate personalized prevention treatment Previous typically failed achieve satisfactory performance, primarily due the use of only a limited number confirmed loci. Here propose that sophisticated machine-learning approaches with large ensemble markers may...
Inflammatory bowel disease, including Crohn's disease (CD) and ulcerative colitis (UC), type 1 diabetes (T1D) are autoimmune diseases that may share common susceptibility pathways. We examined known loci for these in a cohort of 1689 CD cases, 777 UC 989 T1D cases 6197 shared control subjects European ancestry, who were genotyped by the Illumina HumanHap550 SNP arrays. identified multiple previously unreported or unconfirmed associations, (ICOSLG TNFSF15) (TNFAIP3) confer risk, (HERC2 IL26)...
We have analyzed the ability of human gamma+/delta+ T cells to recognize a nominal antigen in association with MHC molecules. A TT-specific cell line approximately 40% was established from hyperimmunized donor, D.F., by stimulation and autologous APC. Three DF-derived clones were CD8+ as determined immunofluorescence staining, Southern Northern blotting probes detecting delta chain rearrangement gamma transcripts, respectively. The responded TT, but not TNP-BSA, APC proliferation IFN-gamma...
OBJECTIVE—Two recent genome-wide association (GWA) studies have revealed novel loci for type 1 diabetes, a common multifactorial disease with strong genetic component. To fully utilize the GWA data that we had obtained by genotyping 563 diabetes probands and 1,146 control subjects, as well 483 case subject–parent trios, using Illumina HumanHap550 BeadChip, designed full stage 2 study to capture other possible signals. RESEARCH DESIGN AND METHODS—From our existing datasets, selected 982...
In stage 1 of our genome-wide association (GWA) study for type diabetes, one locus at 16p13 was detected (P = 1.03 x 10(-10)) and confirmed in two additional cohorts. Here we describe the results testing, these cohorts, 23 loci that were next rank statistical significance.Two independent cohorts studied. The Type Diabetes Genetics Consortium replication cohort consisted 549 families with least child diagnosed diabetes (946 total affected) DNA from both parents. Canadian 364 nuclear family...
Immunotherapy directed against private tumor neo-antigens derived from non-synonymous somatic mutations is a promising strategy of personalized cancer immunotherapy. However, feasibility in low mutational load types remains unknown. Comprehensive and deep analysis circulating tumor-infiltrating lymphocytes (TILs) for neo-epitope specific CD8
Nanopores are promising for many applications including DNA sequencing and molecular filtration. Solid-state nanopores preferable over their biological counterparts requiring durability operation under a wider range of external parameters, yet few studies have focused on optimizing robustness. We report the lifetime pores porous arrays in 10 to 100 nm-thick, low-stress silicon nitride (SiNx) membranes. Pores fabricated using transmission electron microscope (TEM) and/or beam lithography...
Drug reaction with eosinophilia and systemic symptoms (DRESS) is a severe, delayed hypersensitivity (DHR). We observed DRESS to inhibitors of interleukin 1 (IL-1) or IL-6 in small group patients Still's disease atypical lung disease. sought characterise features compared drug-tolerant controls. analysed human leucocyte antigen (HLA) alleles for association inhibitor-related DHR, including Kawasaki (KD) cohort.
Abstract Atopy, which predisposes individuals to develop asthma, severe systemic anaphylaxis, and atopic dermatitis, is usually associated with dramatically elevated total serum IgE levels thought be controlled by a major susceptibility gene multiple minor genes. A recent sib-pair analysis revealed tight linkage between markers on 5q31.1 controlling levels. Due its location within this cluster biologic role in Ig class switching Th2 cell differentiation, the IL-4 has emerged as one candidate...
Although of fundamental importance in developmental biology, the genetic basis for symmetry breaking events that polarize vertebrate oocyte and egg are largely unknown. In vertebrates, first morphological asymmetry is Balbiani body, a highly conserved, transient structure found vertebrates invertebrates including Drosophila, Xenopus, human, mouse. We report identification zebrafish magellan (mgn) mutant, which exhibits novel enlarged body phenotype disruption polarity. To determine molecular...
This study presents performance specifications of an in-house developed human leukocyte antigen (HLA) typing assay using next-generation sequencing (NGS) on the Illumina MiSeq platform. A total 253 samples, previously characterized for HLA-A, -B, -C, -DRB1 and -DQB1 were included in this study, which typed at high-resolution a combination Sanger sequencing, sequence-specific primer (SSP) oligonucleotide probe (SSOP) technologies recorded two-field level. Samples selected with alleles that...
Abstract Autoimmune diseases (AIDs) are polygenic affecting 7–10% of the population in Western Hemisphere with few effective therapies. Here, we quantify heritability paediatric AIDs (pAIDs), including JIA, SLE, CEL, T1D, UC, CD, PS, SPA and CVID, attributable to common genomic variations (SNP -h 2 ). SNP- h estimates most significant for T1D (0.863±s.e. 0.07) JIA (0.727±s.e. 0.037), more modest UC (0.386±s.e. 0.04) CD (0.454±0.025), largely consistent generally greater than that previously...
Human leucocyte antigen (HLA) genes play an important role in the success of organ transplantation and are associated with autoimmune infectious diseases. Current DNA-based genotyping methods, including Sanger sequence-based typing (SSBT), have identified a high degree polymorphism. This level polymorphism makes high-resolution HLA challenging, resulting ambiguous results due to inability resolve phase and/or defining polymorphisms lying outside region amplified. Next-generation sequencing...
The ability to capture and sequence large contiguous DNA fragments represents a significant advancement towards the comprehensive characterization of complex genomic regions. While emerging sequencing platforms are capable producing several kilobases-long reads, fragment sizes generated by current target enrichment technologies remain limiting factor, generally shorter than 1 kbp. methodology described herein, Region-Specific Extraction (RSE), produces segments in excess 20 kbp length....