A5070752105- Metabolomics and Mass Spectrometry Studies
- Nutritional Studies and Diet
- Nitric Oxide and Endothelin Effects
- Reproductive Biology and Fertility
- Sodium Intake and Health
- RNA Research and Splicing
- Chemical synthesis and alkaloids
- Eicosanoids and Hypertension Pharmacology
- Hormonal Regulation and Hypertension
- Molecular Biology Techniques and Applications
- Plant tissue culture and regeneration
- Diet, Metabolism, and Disease
- Viral Infections and Immunology Research
- Redox biology and oxidative stress
- Ovarian function and disorders
- Analytical Chemistry and Chromatography
- Cytokine Signaling Pathways and Interactions
- Thermoregulation and physiological responses
- Gut microbiota and health
- Ginseng Biological Effects and Applications
- Allelopathy and phytotoxic interactions
- Immune Response and Inflammation
- Cell Image Analysis Techniques
- Traditional Chinese Medicine Studies
- Diet and metabolism studies
Peking University Third Hospital
2020-2022
Ministry of Education of the People's Republic of China
2020-2022
Peking University
2020-2022
Beijing Obstetrics and Gynecology Hospital
2020
Xi'an Jiaotong University
2014-2019
Northwest University
2009-2011
Miles College
1974
Fumarase catalyzes the interconversion of fumarate and L-malate in tricarboxylic acid cycle. The Dahl salt-sensitive (SS) rat, a model hypertension, exhibits fumarase insufficiencies. To investigate mechanism mediating effect fumarase-related metabolites on we considered pathway which can be converted to oxaloacetate, aspartate, argininosuccinate, L-arginine, substrate nitric oxide (NO) synthase. levels citrulline, NO were significantly decreased kidneys SS rats compared salt-insensitive...
Abstract Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disorder in women of reproductive age. Some studies have investigated alterations plasma follicular fluid from PCOS patients, but they did not control for obesity or insulin resistance (IR); additionally, correlation analysis metabolites sparse. Accordingly, this study, we aimed to examine differences owing the pathogenesis PCOS, identify hub investigate its associations with androgens. We applied GC–MS platform coupled...
Dietary salt intake has significant effects on arterial blood pressure and the development of hypertension.Mechanisms underlying salt-dependent changes in remain poorly understood, it is difficult to assess salt-sensitivity clinically.Methods: We examined urinary levels metabolites 103 participants Approaches Stop Hypertension (DASH)-Sodium trial after nearly 30 days a defined diet containing high sodium (targeting 150 mmol per day) or low (50 day).Targeted chromatography/mass spectrometry...
Different strategies of ovarian stimulation are widely used in IVF to retrieve mature metaphase II (MII) oocytes for fertilization. On average, approximately 70% recovered mature, while personalized administration hCG and/or GnRH agonist trigger and vitro maturation (IVM) management can further improve the rate. However, even under such conditions, a complete absence oocyte is still observed sporadically. The probable causes maturation-deficient (MD) – which arrest abnormally at I (MI) stage...
The antiviral activity induced in mice by MA-56 has been characterized as an interferon. Maximum serum concentration is attained within 16-18 hr after feeding a single oral dose ranging from 400 to 500 mg/kg body wt. induces hyporeactive period of at least 40 hr. Interferon-production kinetics and cycloheximide experiments indicate that MA-56, like tilorone, induction pattern can be blocked resembles viral rather than synthetic polynucleotides bacterial endotoxin. Although apparently does...
Fumarase catalyzes the interconversion of fumarate and l-malate in tricarboxylic acid cycle. insufficiencies were associated with increased levels fumarate, decreased malate exacerbated salt-induced hypertension. To gain insights into metabolism profiles induced by fumarase insufficiency identify key regulatory metabolites, we applied a GC-MS based metabolomics platform coupled network approach to analyze insufficient human umbilical vein endothelial cells (HUVEC) negative controls. A total...
Abstract Dahl SS rats exhibit greater levels of renal medullary oxidative stress and lower fumarase activities. Fumarase insufficiencies can increase reactive oxygen species (ROS), the mechanism which, however, is not clear. A proteomic analysis indicated knockdown in HK-2 cells resulted changes expression or activity NADPH oxidase, mitochondrial respiratory chain complex I III, ATP synthase subunits, α-oxoglutarate dehydrogenase, all which are sites ROS formation. Meantime, activities key...
Abstract High‐salt diets (HSDs) are associated with elevated levels of reactive oxygen species (ROS), which play a key role in ovarian disorders. However, it is not yet clear whether HSDs impact redox balance and metabolism. Accordingly, this study, we analyzed the effect on by biochemical analysis further dissected its possible mechanism metabolic combined correlation network method. We found that ROS H 2 O significantly increased tissue mice receiving an HSD for 4 weeks. The enhanced...
The awareness, treatment and controls rates of hypertension for people in their 20s 30s age are much lower than average. In this paper, a GC/MS based metabolomics study was performed plasma young hypertensive men age-matched normal ones. Correlations the identified metabolites were analyzed visualized. A systematic correlation network constructed with significance coefficient setting at threshold 0.6. Glycine, lysine, cystine beta-alanine selected as most important nodes network, high values...
The purpose of this research work was to investigate whether such a workflow including the phosphoproteins enriched by affinity purification, isolated and quantified two-dimensional difference gel electrophoresis, differential protein spots identified mass spectrometry, suitable dissect intact phosphoprotein profile that mediated cell signaling pathways. Endothelial cells with or without vascular endothelial growth factor (VEGF) induction were used in experiment. accidental error introduced...
Our previous proteomic study revealed substantial differences in several proteins between Dahl salt-sensitive (SS) rats and salt-insensitive consomic SS.13BN rats. Subsequent studies indicated a role of fumarase insufficiency the development hypertension SS A global metabolic profiling using gas chromatography/mass spectrometry (GC/MS) was performed plasma (n=9) (n=8) on 0.4% NaCl diet to gain further insights into relationship alterations cellular intermediary metabolism predisposition...
To study tissue culture of Vinca minor and determine the content vincamine.Leaf blades, stalks, root segment V. were used as explants to effect 2, 4-D,6-BA,NAA on its callus induction vincamine contents in orthogonal design experiment. In peak period formation, sterile plants was determined by HPLC. The experimental data statistically analyzed.The 6-BA NAA had no significant induction. But 4-D Within 20,40,60 d, plant (0.015 +/- 0.003)%, (0.097 0.001)% , (0.113 0.06)%, respectively. leaf...
The retrieval of the existing literature on types fungi polysaccharides, chemical structure and anti-canceractivity, anti-cancer mechanism was outlined, providing scientific information for further study action polysaccharides fungi. Key words: Polysaccharides; Chemical structure; Anti-tumoractivity; Mechanism
Dietary salt intake has significant effects on arterial blood pressure and the development of hypertension. Mechanisms underlying salt-dependent changes in remain poorly understood. The goal present study was to utilize urine samples from Approaches Stop Hypertension (DASH)-Sodium trial a targeted metabolomic approach identify associations urinary metabolites with phenotypes low- or high-sodium intake. We examined levels 103 participants DASH-Sodium after 30 days defined diet containing high...