A5083426937- Liver Disease Diagnosis and Treatment
- Alcohol Consumption and Health Effects
- Eicosanoids and Hypertension Pharmacology
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Gut microbiota and health
- Fatty Acid Research and Health
- Metabolomics and Mass Spectrometry Studies
- Endoplasmic Reticulum Stress and Disease
- Liver Disease and Transplantation
- Alcoholism and Thiamine Deficiency
- Cancer, Hypoxia, and Metabolism
- Biochemical Acid Research Studies
- Liver Diseases and Immunity
- Drug-Induced Hepatotoxicity and Protection
- Folate and B Vitamins Research
- Inflammatory mediators and NSAID effects
- Barrier Structure and Function Studies
- Cancer, Lipids, and Metabolism
- Biochemical effects in animals
- Hepatitis C virus research
- Human Health and Disease
- Histone Deacetylase Inhibitors Research
- Fibroblast Growth Factor Research
- Clinical Nutrition and Gastroenterology
University of Louisville
2016-2025
University of Louisville Hospital
2011-2024
Louisiana Tech University
2024
Louisville VA Medical Center
2011-2019
Nebraska Medical Center
2016
University of Nottingham
2016
Royal Derby Hospital
2016
University of Colorado Denver
2016
Cedars-Sinai Medical Center
2016
VA Nebraska Western Iowa Health Care System
2016
Enteric dysbiosis plays an essential role in the pathogenesis of alcoholic liver disease (ALD). Detailed characterization alterations gut microbiome is needed for understanding their pathogenic ALD and developing effective therapeutic approaches using probiotic supplementation. Mice were fed liquid Lieber-DeCarli diet without or with alcohol (5% v/v) 6 weeks. A subset mice administered Lactobacillus rhamnosus GG (LGG) from to 8 Indicators intestinal permeability, hepatic steatosis,...
Gut-derived endotoxin is a critical factor in the development and progression of alcoholic liver disease (ALD). Probiotics can treat alcohol-induced injury associated with gut leakiness endotoxemia animal models, as well human ALD; however, mechanism or mechanisms their beneficial action are not defined. We hypothesized that alcohol impairs adaptive response-induced hypoxia-inducible (HIF) probiotic supplementation could attenuate this impairment, restoring barrier function mouse model ALD...
Background: Interactions between the gut, immune system, and liver, as well type of fat in diet, are critical components alcoholic liver disease (ALD). The goal present study was to determine effects saturated (SF) unsaturated (USF) on ethanol (EtOH)‐induced gut‐liver interactions a mouse model ALD. Methods: C57BL/6N mice were fed Lieber–DeCarli liquid diets containing EtOH enriched USF (corn oil) or SF (medium chain triglycerides:beef tallow). Control pair‐fed an isocaloric basis. Liver...
Circulating oxidized linoleic acid (LA) metabolites (OXLAMs) are increased in patients with nonalcoholic steatohepatitis (NASH) and their levels correlate disease severity. However, the mechanisms by which OXLAMs contribute to NASH development incompletely understood. We tested hypothesis that LA or provided directly through diet involved of hepatic injury. C57BL/6 mice were fed an isocaloric high-fat containing low LA, high for 8 weeks. The livers OXLAM-fed showed lower triglyceride...
Background Interactions between the liver, gut, and immune system are critical components of alcoholic liver disease (ALD). The aim this study was to explore associations alcohol‐induced injury, endotoxemia, inflammation at admission over time during abstinence, as well examine sex‐related differences in these parameters alcohol‐dependent individuals admitted an alcohol treatment program. Methods A cohort 48 otherwise healthy participants with use disorder, but no clinical signs injury (34...
Background Binge, as well chronic, alcohol consumption affects global histone acetylation leading to changes in gene expression. It is becoming increasingly evident that these histone‐associated epigenetic modifications play an important role the development of alcohol‐mediated hepatic injury. Methods C 57 BL /6 mice were gavaged 3 times (12‐hour intervals) with ethanol ( EtOH ; 4.5 g/kg). Hepatic deacetylase Hdac ) m RNA s assessed by q RT ‐ PCR . Total HDAC activity was estimated a...
Ethanol (EtOH)-induced alterations in intestinal homeostasis lead to multi-system pathologies, including liver injury. ω-6 PUFAs exert pro-inflammatory activity, while ω-3 promote anti-inflammatory activity that is mediated, part, through specialized pro-resolving mediators [e.g., resolvin D1 (RvD1)]. We tested the hypothesis a decrease ω-6:ω-3 PUFA ratio would attenuate EtOH-mediated gut-liver axis. FA desaturase-1 (fat-1) mice, which endogenously increase levels, were protected against...
During the coronavirus disease 2019 (COVID-19) pandemic, there was a marked increase in alcohol consumption. COVID-19 superimposed on underlying liver notably worsens outcome of many forms injury. The goal current pilot study to test dual exposure and infection an experimental animal model alcohol-associated (ALD).
Polychlorinated biphenyls (PCBs) are persistent organic pollutants associated with non-alcoholic fatty liver disease (NAFLD). Previously, we demonstrated that the PCB mixture, Aroclor1260, exacerbated NAFLD, reflective of toxicant-associated steatohepatitis, in diet-induced obese mice, part through pregnane-xenobiotic receptor (PXR) and constitutive androstane (CAR) activation. Recent studies have also reported PCB-induced changes gut microbiome consequently impact NAFLD. Therefore,...
<i>S</i>-Adenosylmethionine (SAM) treatment has anti-inflammatory, cytoprotective effects against endotoxin-induced organ injury. An important component of the anti-inflammatory action SAM involves down-regulation lipopolysaccharide (LPS)-induced transcriptional induction tumor necrosis factor-α (TNF) expression by monocytes/macrophages. We examined effect on and activity LPS-induced up-regulation phosphodiesterase 4 (PDE4), which regulates cellular cAMP levels TNF expression. LPS RAW 264.7,...