A5026087539- Alcohol Consumption and Health Effects
- Liver Disease Diagnosis and Treatment
- Eicosanoids and Hypertension Pharmacology
- Diet, Metabolism, and Disease
- Fatty Acid Research and Health
- Liver Disease and Transplantation
- Gut microbiota and health
- Diet and metabolism studies
- Endoplasmic Reticulum Stress and Disease
- Liver Diseases and Immunity
- Drug-Induced Hepatotoxicity and Protection
- Metabolomics and Mass Spectrometry Studies
- Diabetes and associated disorders
- CRISPR and Genetic Engineering
- Fibroblast Growth Factor Research
- Natural Products and Biological Research
- Clostridium difficile and Clostridium perfringens research
- Adipokines, Inflammation, and Metabolic Diseases
- COVID-19 Clinical Research Studies
- Probiotics and Fermented Foods
- Hepatitis C virus research
- Connective tissue disorders research
- Cancer, Hypoxia, and Metabolism
- Neuropeptides and Animal Physiology
- Immune cells in cancer
University of Louisville
2018-2025
National Institute of Environmental Health Sciences
2023
National Institutes of Health
2023
University of Louisville Hospital
2020
University of Florida
2019
Ethanol (EtOH)-induced alterations in intestinal homeostasis lead to multi-system pathologies, including liver injury. ω-6 PUFAs exert pro-inflammatory activity, while ω-3 promote anti-inflammatory activity that is mediated, part, through specialized pro-resolving mediators [e.g., resolvin D1 (RvD1)]. We tested the hypothesis a decrease ω-6:ω-3 PUFA ratio would attenuate EtOH-mediated gut-liver axis. FA desaturase-1 (fat-1) mice, which endogenously increase levels, were protected against...
During the coronavirus disease 2019 (COVID-19) pandemic, there was a marked increase in alcohol consumption. COVID-19 superimposed on underlying liver notably worsens outcome of many forms injury. The goal current pilot study to test dual exposure and infection an experimental animal model alcohol-associated (ALD).
Alcohol-associated liver disease (ALD) is a prevalent disorder and significant global healthcare burden with limited effective therapeutic options. The gut-liver axis critical factor contributing to susceptibility injury due alcohol consumption. In the current study, we tested whether human beta defensin-2 (hBD-2), small anti-microbial peptide, attenuates experimental chronic ALD. Male C57Bl/6J mice were fed an ethanol (EtOH)-containing diet for 6 weeks daily administration of hBD-2 (1.2...
Background: Alcohol-associated hepatitis (AH) is one of the clinical presentations alcohol-associated liver disease. AH has poor prognosis, and corticosteroids remain mainstay drug therapy. However, ~40% patients do not respond to this treatment, mechanisms underlying altered response are understood. The current study aimed identify changes in hepatic protein expression associated with responsiveness prognosis AH. Methods: Patients were enrolled based on National Institute Alcohol Abuse...
Alcoholic liver disease (ALD), a significant health problem, progresses through the course of several pathologies including steatosis, steatohepatitis, fibrosis, and cirrhosis. There are no effective FDA-approved medications to prevent or treat any stages ALD, mechanisms involved in ALD pathogenesis not well understood. Bioactive lipid metabolites play crucial role numerous pathological conditions, as induction resolution inflammation. Herein, hepatic lipidomic analysis was performed on...
Alcoholic liver disease (ALD) comprises a spectrum of pathology, including steatosis, steatohepatitis, and cirrhosis. Previous work from our group others suggests that dietary fat, both the amount composition, plays pivotal role in ALD development progression; however, impact specific fatty acids on pathogenesis is not fully elucidated. Preclinical rodent models revealed deleterious effects omega-6 polyunsaturated (n-6 PUFAs), specifically linoleic acid (LA), this may be partially attributed...
Alcohol‐associated liver disease (ALD) is a spectrum of disorders ranging from steatosis to steatohepatitis, fibrosis, and cirrhosis. hepatitis (AH) an acute often severe form ALD with substantial morbidity mortality. The mechanisms mediators progression severity are not well understood, effective therapeutic options limited. Various bioactive lipid have recently emerged as important factors in pathogenesis. current study aimed examine alterations linoleic acid (LA)‐derived metabolites the...
Alcohol-associated liver disease (ALD) is a major human health issue for which there are limited treatment options. Experimental evidence suggests that nutrition plays an important role in ALD pathogenesis, and specific dietary fatty acids, example, n6 or n3-PUFAs, may exacerbate attenuate ALD, respectively. The purpose of the current study was to determine whether beneficial effects n3-PUFA enrichment were mediated, part, by improvement Wnt signaling. Wild-type (WT) fat-1 transgenic mice...
Alcohol-associated liver disease (ALD) is a major health problem with limited effective treatment options. hepatitis (AH) subset of severe ALD high rate mortality due to infection, inflammation, and ultimately multi-organ failure. There an urgent need for novel therapeutic approaches alleviate the human suffering associated this condition. Resolvin D1 (RvD1) promotes resolution inflammation regulates immune responses. The current study aimed test efficacy mechanisms RvD1-mediated effects on...
Abstract Background Alcohol use is a major global healthcare burden that contributes to numerous adverse health outcomes, including liver disease. Many factors influence individual susceptibility alcohol‐associated diseases, nutritional factors. The objective of the current study was examine inter‐relations among alcohol, dietary micronutrients and macronutrient consumption, by analyzing data from 2017–2018 National Health Nutrition Examination Survey (NHANES). Methods Based on self‐reported...
Alcohol-associated liver disease (ALD) is a prevalent medical problem with limited effective treatment strategies. Although many biological processes contributing to ALD have been elucidated, complete understanding of the underlying mechanisms still lacking. The current study employed proteomic approach identify hepatic changes resulting from ethanol (EtOH) consumption and genetic ablation formyl peptide receptor 2 (FPR2), G-protein coupled known regulate multiple signaling pathways...
Alcohol-associated liver disease (ALD) is the most common chronic and carries a significant healthcare burden. ALD has no long-term treatment options aside from abstinence, mechanisms that contribute to its pathogenesis are not fully understood. This study aimed investigate role of formyl peptide receptor 2 (FPR2), for immunomodulatory signals, in ALD. WT Fpr2−/− mice were exposed chronic–binge ethanol administration subsequently assessed injury, inflammation, markers regeneration. The...
Alcohol-associated liver disease (ALD) is the leading cause of worldwide, and alcohol-associated hepatitis (AH), a severe form ALD, major contributor to mortality morbidity due ALD. Many factors modulate susceptibility ALD development progression, including nutritional such as dietary fatty acids. Recent work from our group others showed that modulation or endogenous levels n6-and n3-polyunsaturated acids (PUFAs) can exacerbate attenuate experimental respectively. In current study, we...
Chronic alcohol consumption leads to disturbances in intestinal function which can be exacerbated by inflammation and modulated different factors, e.g., polyunsaturated fatty acids (PUFAs). The mechanisms underlying these alterations are not well understood. In this study, RNA-seq analysis was performed on ileum tissue from WT fat-1 transgenic mice (which have elevated endogenous n-3 PUFAs). Mice were chronically fed ethanol (EtOH) challenged with a single lipopolysaccharide (LPS) dose...
Abstract The intestine interacts with many factors, including dietary components and ethanol (EtOH), which can impact intestinal health. Previous studies showed that different types of fats modulate EtOH-induced changes in the intestine; however, mechanisms underlying these effects are not completely understood. Here, we examined transcriptional responses to EtOH WT transgenic fat-1 mice (which endogenously convert n6 n3 polyunsaturated fatty acids [PUFAs]) identify novel genes pathways...
Introduction Cytochrome P450 epoxygenases are important enzymes involved in the conversion of polyunsaturated fatty acids (PUFAs) into epoxides, which have been shown to beneficial effects a number inflammation‐related diseases. However, vivo half‐lives these epoxides is short, due action soluble epoxide hydrolase (sEH), an enzyme responsible for converting their bioactive dihydroxy metabolites. sEH inhibitors, such as trans‐4‐{4‐[3‐(4‐trifluoromethoxyphenyl)‐ureido] cyclohexyloxy} benzoic...