A5066713924- Plant-Microbe Interactions and Immunity
- Bacterial Genetics and Biotechnology
- Microbial Community Ecology and Physiology
- Bacterial biofilms and quorum sensing
- Plant Pathogenic Bacteria Studies
- Microbial Natural Products and Biosynthesis
- Wind and Air Flow Studies
- Fluid Dynamics and Vibration Analysis
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Solar Energy Systems and Technologies
- Bacteriophages and microbial interactions
- Amino Acid Enzymes and Metabolism
- Protein Hydrolysis and Bioactive Peptides
- Fungal Biology and Applications
- Microbial Metabolic Engineering and Bioproduction
- Wastewater Treatment and Nitrogen Removal
- Plant Pathogens and Fungal Diseases
- Seismic Performance and Analysis
- Antibiotic Resistance in Bacteria
- Adsorption and Cooling Systems
- Insect symbiosis and bacterial influences
- Biochemical effects in animals
- GABA and Rice Research
- Plant tissue culture and regeneration
Tongji University
2015-2024
Ludong University
2006-2022
Nanjing Agricultural University
2019
Louisiana State University
2013
Shanghai Jiao Tong University
2004-2007
Huaiyin Normal University
2006-2007
Gene duplication often provides selective advantages for the survival of microorganisms in adapting to varying environmental conditions. P. aeruginosa PAO1 possesses two seven-gene operons [phz1 (phzA1B1C1D1E1F1G1) and phz2 (phzA2B2C2D2E2F2G2)] that are involved biosynthesis phenazine-1-carboxylic acid its derivatives. Although highly homologous their functions well known, it is unclear how phz coordinate expressions maintain phenazine biosynthesis. By constructing single double deletion...
The biosynthesis of antimicrobial metabolites is controlled by the GacS/GacA two-component regulatory system in Pseudomonas species. production phenazine-1-carboxylic acid and pyoluteorin differentially regulated GacA sp. M18. Pyoluteorin was reduced to nondetectable level culture gacA insertional mutant strain M18G grown King's medium B broth, whereas increased 30-fold over that wild-type strain. Production both antibiotics restored levels after complementation trans with gene. Expression...
A new regulator gene named pltZ, which is located downstream of the plt cluster in genome Pseudomonas sp. M18, was identified, sequenced and characterized this report. The deduced amino acid sequence PltZ shares significant homology with other bacterial regulators TetR family. chromosomal pltZ disruption mutant gave rise to 4.4-fold enhancement pyoluteorin biosynthesis but did not exert influence on accumulation phenazine-1-carboxylic compared wild-type M18. negative regulation further...
The rigid pressure measurement and aero–elastic vibration were performed to the largest exhaust cooling tower in Asia. Through analyses, surface wind distribution, multitower proportional coefficient, wind-induced coefficient of towers from most unfavorable direction obtained. On this basis, finite-element software self-made repreprocessing postprocessing programs employed analyze ultimate load-carrying capacity overall local stabilities three conditions, which include (1) no opening, (2)...
RpoS, one of the two alternative σ factors in Borrelia burgdorferi, is tightly controlled by multiple regulators and, turn, determines expression many critical virulence factors. Here we show that increasing RpoS causes cell death. The immediate effect was to promote bacterial division and as a consequence result rapid increase number before causing No DNA fragmentation or degradation observed during this induced Cryo-electron microscopy showed cells first formed blebs, which were eventually...
To investigate the regulatory mechanism governing antifungal metabolite biosynthesis, two kinds of global regulator genes in Pseudomonas sp. M18, an rpoS and rsmA gene, were cloned mutated by inserting with aacC1 cassette, respectively. Two mutants showed same mode repression phenazine-1-carboxylic acid remarkable enhancement pyoluteorin. In rpoS-mutant, a translational rsmA'-'lacZ fusion was expressed at level corresponding to that wild-type strain. rsmA-mutant, however, expression...
This study aimed to develop an approach accurately predict the wind models and effects of large turbines. The wind-induced vibration characteristics a 5 MW tower-blade coupled turbine system have been investigated in this paper. First, blade-tower integration model was established, which included blades, nacelle, tower base system. harmonic superposition method modified blade element momentum theory were then applied simulate fluctuating field for rotor blades tower. Finally, responses...
The traditional method for the simulation of high Reynolds number (Re) effects on wind loads cooling tower models in tunnels focuses only mean pressure distribution. Based observed some key factors static/dynamic flow characteristics around towers, study reported this paper describes a comprehensive using both and fluctuating distributions at Re as targets, which is indispensable obtaining complete full-scale tunnels. After being presented case study, proposed examined by comparing full...
In previous studies with Pseudomonas chlororaphis G05, two operons ( phzABCDEFG and prnABCD ) were confirmed to respectively encode enzymes for biosynthesis of phenazine‐1‐carboxylic acid pyrrolnitrin that mainly contributed suppression some fungal phytopathogens. Although regulators identified govern their expression, it is not known how coordinately interact. By constructing the phz ‐ or/and prn‐ deletion mutants, we found in comparison wild‐type strain production mutant G05Δ prn obviously...
Abstract Pseudomonas aeruginosa PAO1, a common opportunistic bacterial pathogen, contains two phenazine‐biosynthetic operons, phz1 ( phzA 1 B C D E F G ) and phz2 2 ). Each of operons can independently encode set enzymes involving in the biosynthesis phenazine‐1‐carboxylic acid. As global transcriptional regulator, RpoS mediates lot genes secondary metabolites many bacteria. In an other previous study, it was reported that deficiency caused overproduction pyocyanin, derivative acid P. PAO1....
In our previous study, pyrrolnitrin produced in Pseudomonas chlororaphis G05 plays more critical role suppression of mycelial growth some fungal pathogens that cause plant diseases agriculture. Although regulators for biosynthesis were identified, the regulation pathway was not fully constructed. During screening novel regulator candidates, we obtained a white conjugant G05W02 while transposon mutagenesis carried out between fusion mutant G05ΔphzΔprn::lacZ and E. coli S17-1...
In our recent work, we found that pyrrolnitrin, and not phenazines, contributed to the suppression of mycelia growth Fusarium graminearum causes heavy head blight (FHB) disease in cereal crops. However, pyrrolnitrin production Pseudomonas chlororaphis G05 King's B medium was very low. Although a few regulatory genes mediating prnABCD (the prn operon, biosynthetic locus) expression have been identified, it is enough for us enhance by systematically constructing genetically-engineered strain....