A5102005073- Receptor Mechanisms and Signaling
- Advanced DC-DC Converters
- Multilevel Inverters and Converters
- Digital Filter Design and Implementation
- Numerical Methods and Algorithms
- Image and Signal Denoising Methods
- Silicon Carbide Semiconductor Technologies
- Neuroscience and Neuropharmacology Research
- Radiation Effects in Electronics
- Neuropeptides and Animal Physiology
- Advanced Image Processing Techniques
- Click Chemistry and Applications
- Computational Drug Discovery Methods
- Advancements in PLL and VCO Technologies
- Electromagnetic Compatibility and Noise Suppression
- Epigenetics and DNA Methylation
- Low-power high-performance VLSI design
- Smart Grid and Power Systems
- Cancer, Lipids, and Metabolism
- Radio Frequency Integrated Circuit Design
- RNA modifications and cancer
- Education and Learning Interventions
- Autonomous Vehicle Technology and Safety
- Error Correcting Code Techniques
- Photochromic and Fluorescence Chemistry
Tsinghua University
2017-2025
University of California, San Francisco
2024-2025
Center for Life Sciences
2019-2024
Shenyang Institute of Engineering
2024
Beijing Advanced Sciences and Innovation Center
2020-2024
Second Affiliated Hospital of Soochow University
2023
Soochow University
2023
Chongqing Medical University
2023
Shanghai Xuhui Central Hospital
2023
Fudan University
2023
The crystal structure of the β2-adrenergic receptor (β2AR) bound to G protein adenylyl cyclase stimulatory (Gs) captured complex in a nucleotide-free state (β2AR-Gsempty). Unfortunately, β2AR-Gsempty does not provide clear explanation for coupling specificity. Evidence from several sources suggests existence transient between β2AR and GDP-bound Gs (β2AR-GsGDP) that may represent an intermediate on way formation contribute Here we present with carboxyl terminal 14 amino acids Gαs along...
Steviol glycosides are the intensely sweet components of extracts from Stevia rebaudiana. These molecules comprise an invariant steviol aglycone decorated with variable glycans and could widely serve as a low-calorie sweetener. However, most desirable Reb D M, devoid unpleasant aftertaste, naturally produced only in trace amounts due to low levels specific β (1-2) glucosylation Stevia. Here, we report biochemical structural characterization OsUGT91C1, glycosyltransferase Oryza sativa, which...
Abstract G protein-coupled receptors (GPCRs) within the same subfamily often share high homology in their orthosteric pocket and therefore pose challenges to drug development. The amino acids that form binding for epinephrine norepinephrine β 1 2 adrenergic (β AR AR) are identical. Here, examine effect of conformational restriction on ligand kinetics, we synthesized a constrained epinephrine. Surprisingly, exhibits over 100-fold selectivity AR. We provide evidence may be due reduced...
GPCRs signal through both G protein pathways and β–arrestin pathways. Previous work suggests that β–arrestins bind to different modes, including core engagement tail engagement. Core competes with proteins terminates signaling, while can coexist proteins, mediating sustained intracellular activation of the receptor – a process dependent on high affinity between phosphorylated C-terminus receptor. In this study, we determined structure GPCR - β-arrestin-1 complex stabilized by an allosteric...
Approximately 1/3 of all clinical drugs exert their therapeutic effects by modulating the activity G protein-coupled receptors (GPCRs). Thus, there is a constant interest in finding novel ways to modulate GPCR activity. In this work, through newly established Survival Pressure Selection (SPS) method for high-throughput screening agonists, we discover that atazanavir functions as an agonist GPR119. Further studies suggest capable activating number Family A GPCRs, including β1 adrenergic...
Significance To investigate the epigenetic mechanism of pregnancy failure in mammals, we exploited high rate fetal loss pig induced pluripotent stem cell (iPSC) nuclear transfer. We generated methylomes iPSCs and associated transfer embryos from reciprocal crosses between two distinct breeds. Our methylome analysis revealed that misregulation RTL1 as principal basis using iPSCs. Remarkably, has broad fertility implications across mouse, rat, pig, cattle, human cloning, tetraploid...
Abstract Virtual libraries for ligand discovery have recently increased 10,000-fold, and this is thought to improved hit rates potencies from library docking. This idea has not, however, been experimentally tested in direct comparisons of larger-vs-smaller libraries. Meanwhile, though exploded, the scale experimental testing little changed, with often only dozens high-ranked molecules investigated, making interpretation affinities uncertain. Accordingly, we docked a 1.7 billion molecule...
A cost-effective totem-pole power factor correction converter with high-frequency gallium nitride high electron-mobility transistors and low-frequency Si diodes is investigated in this article. The reverse recovery characteristics of are evaluated experimentally. With the slow characteristics, a negative current can flow through low frequency diode, so that soft-switching critical mode operation achieved easily. However, if duration exceeds time diode will switch at frequency, leading to...
Nanopore sequencing workflows have attracted increasing attention owing to their fast, real-time, and convenient portability. Positive blood culture samples were collected from patients with bacterial bloodstream infection tested by nanopore sequencing. This study compared the results for pathogen taxonomic profiling antimicrobial resistance genes those of species identification phenotypic drug susceptibility using traditional microbiology testing. A total 37 positive strain genotyping...
Abstract Since 10,000 BC, continuous human selection has led to intense genetic and phenotypic changes in pig ( Sus scrofa ) domestication. Through whole genome analysis of 257 individuals, we demonstrated artificial unidirectional bidirectional as the primary force shape convergent divergent between Chinese domestic pigs (CHD) European (EUD). We identified 31 genes regions that might be related fundamental domestication requirements pigs. And these belong predominantly categories nervous...
Metastable binding sites (MBS) have been observed in a multitude of molecular dynamics simulations and can be considered low affinity allosteric (ABS) that function as stepping stones the ligand moves toward orthosteric site (OBS). Herein, we show MBS utilized ABS design, resulting ligands with improved kinetics. Four homobivalent bitopic (1–4) were designed by docking (S)-alprenolol ((S)-ALP) cocrystal structure β2 adrenergic receptor (β2AR) bound to antagonist ALP. Ligand 4 displayed...
This paper describes a bias-current-free fractional-N hybrid phase-locked loop (HPLL) architecture that does not use charge pump (CP) or linear time-to-digital converter (TDC). A control with digital integral path and an analog proportional-gain offers technology scalability as well phase detection under low supply voltage. The CP-less consists of flip-flop detector (PD) passive filters including programmable notch filter. Unlike the TDC, PD has negligible contribution to in-band noise over...
In this paper, the analysis and design of an optimized asymmetrical half-bridge DC-DC converter (AHBC) is presented. The optimal turns ratios for transformer are achieved limited duty ratio range. order to ensure realization ZVS in operation, required interlock delay time switches gate signals leakage inductance properly calculated. considerations discussed, results from simulation experiment, which verify analysis, also With actual operation AHBC can be predicted practical applications.
In this paper, a configurable FFT processor is presented which can be configured as 8192 point, 4096 2048 point and 1024 processor. The based on mixed radix algorithm single-path delay feedback(SDF) architecture adopted. achieved by connecting or bypassing specific processing elements. To improve performance, dynamic scaling approach adopted internal data formatted self-defined floating the arithmetic for simple. experiment results show that achieve high constant SNR. implemented FPGA.
Abstract To mitigate the sensitivity of charge pump in a traditional Phase-Locked Loop(PLL), single-event-hardened PLL architecture with proportional and integral path is proposed. The phase margin kept at 58.16° due to rational design output clock frequency ranges from 0.8 3.2 GHz. circuit-level simulation results reveal that sensitive volume hardened decreases by 80% ∼ 95%. novel radiation-hardened circuit was implemented 28 nm CMOS technology irradiated heavy ions linear energy transfer...
In this paper, the analysis and design of an asymmetrical half bridge flyback DC-DC converter is presented, which can minimize switching power loss by realizing zero-voltage (ZVS) during transition between two switches zero-current-switching (ZCS) on output diode. As a result, high efficiency be achieved. The principle operation explained analyzed. order to ensure realization ZVS in operation, required interlock delay time gate signals switches, transformer leakage inductance, range current...
The Cache plays an important role in computer architecture by reducing the access time of processor and improving its performance. hardware design is complex it challenging to verify functions, so traditional Verilog-based verification method no longer applicable. This paper proposes a comprehensive efficient testbench based on SystemVerilog language universal methodology (UVM) for instruction (I-Cache) controller. Corresponding testcases are designed each feature I-Cache controller...
In this paper efficient hardware architecture for deblocking filter in H.264 is presented. A parallel filtering order proposed without violating the standard, and implemented pipelined dataptah. With vertical edges horizontal can be processed simutaneously, efficiency improved. The memory arranged orgnized carefully, data reused efficiently reference reduced. verified on FPGA, it takes only 116 clock cycle to process a 4:2:0 macroblock.