A5022775237- Metal Extraction and Bioleaching
- Corrosion Behavior and Inhibition
- Bacterial biofilms and quorum sensing
- Microbial Fuel Cells and Bioremediation
- Wastewater Treatment and Nitrogen Removal
- Mine drainage and remediation techniques
- Crystallization and Solubility Studies
- Chromium effects and bioremediation
- X-ray Diffraction in Crystallography
- Adsorption and biosorption for pollutant removal
- Microbial Community Ecology and Physiology
- Chemical and Physical Properties in Aqueous Solutions
- Extraction and Separation Processes
- Anaerobic Digestion and Biogas Production
- Diet, Metabolism, and Disease
- Advanced Cellulose Research Studies
- Constructed Wetlands for Wastewater Treatment
- Minerals Flotation and Separation Techniques
- Botanical Research and Applications
- Biofuel production and bioconversion
- Sulfur Compounds in Biology
- Quinazolinone synthesis and applications
- Amino Acid Enzymes and Metabolism
- Bacterial Genetics and Biotechnology
- Enzyme function and inhibition
Shandong University
2014-2025
State Key Laboratory of Microbial Technology
2013-2024
Guangxi Medical University
2024
Qinghai Provincial Peoples Hospital
2019-2023
Qingdao Binhai University
2020
Louisiana State University
2013
Acidithiobacillus caldus (A. caldus) is widely used in bio-leaching. It gains energy and electrons from oxidation of elemental sulfur reduced inorganic compounds (RISCs) for carbon dioxide fixation growth. Genomic analyses suggest that its system involves a truncated (Sox) (omitting SoxCD), non-Sox similar to the A. ferrooxidans, oxygenase reductase (SOR). The complexity generates big obstacle on research mechanism. However, development genetic manipulation method recent years provides...
Microbial nest system (MNS) represents a novel and efficient approach to treating solid-liquid mixture from pig farming instead of the conventional method, which separates solid liquid at first by using centrifugation then treats respectively. However, key environmental factors influencing efficiency this microbial structure are still not clear. This study aimed elucidate changes MNS in physicochemical properties, spectral analysis, correlations between community structures during treatment....
Chromium contamination has been an increasing threat to the environment and human health. Cr(VI) Cr(III) are most common states of chromium. However, compared with Cr(III), is more toxic easily absorbed, therefore, it harmful beings. Thus, conversion into accepted strategy for chromium detoxification. Here, we isolated two Bacillus cereus strains a high tolerance reduction ability, named B. D 332, respectively. Both demonstrated strong pH temperature adaptability survival under 8 mM Cr(VI)....
Acidithiobacillus ferrooxidans is an acidophilic and chemolithotrophic sulfur- iron-oxidizing bacterium that has been widely used in the bioleaching process for extracting metals. Extracellular polymeric substances (EPS) are essential bacteria-ore interactions, regulation of EPS synthesis could be important way influencing efficiency process. Therefore, exploring utilizing regulatory pathways to improve bacterial capability have posed a challenge study application bacteria. Here, several...
Acidithiobacillus caldus (A. caldus) is a common bioleaching bacterium that possesses sophisticated and highly efficient inorganic sulfur compound metabolism network. Thiosulfate, central intermediate in the network of A. other sulfur-oxidizing microorganisms, can be metabolized via tetrathionate (S4I) pathway catalyzed by thiosulfate:quinol oxidoreductase (Tqo or DoxDA) hydrolase (TetH). In caldus, there an additional two-component system called RsrS-RsrR. Since rsrS rsrR are arranged as...
Acidithiobacillus caldus is a chemolithoautotrophic sulfur-oxidizing bacterium that widely used for bioleaching processes. spp. are suggested to contain sulfur dioxygenases (SDOs) facilitate oxidation. In this study, two putative sdo genes (A5904_0421 and A5904_1112) were detected in the genome of A. MTH-04 by BLASTP searching with previously identified SDO (A5904_0790). We cloned expressed these genes, activity recombinant protein A5904_0421 GSH-dependent vitro assay. Phylogenetic analysis...
Effect of imatinib on rats with spinal cord injury (SCI) was investigated through the nuclear factor erythroid 2-related 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. Forty-eight Sprague-Dawley were randomly divided into sham operation group (n=12), model (n=12) and inhibitor (n=12). The results immunohistochemistry showed that in comparison group, other three groups had overtly increased positive expression level Bax evidently reduced Bcl-2 (P<0.05). Compared those distinctly lower,...
Acidophiles play a dominant role in driving elemental cycling natural acid mine drainage (AMD) habitats and exhibit important application value bioleaching bioremediation. Acidity is an inevitable environmental stress key factor that affects the survival of acidophiles their acidified habitats; however, regulatory strategies applied by acidophilic bacteria to withstand low pH are unclear. We identified significance ferric uptake regulator (Fur) adapting acidic environments discovered Fur...
A LuxI/R-like quorum sensing (QS) system (AfeI/R) has been reported in the acidophilic and chemoautotrophic Acidithiobacillus spp. However, function of AfeI/R remains unclear because difficulties genetic manipulation these bacteria. Here, we constructed different afeI mutants sulfur- iron-oxidizer A. ferrooxidans, identified N-acyl homoserine lactones (acyl-HSLs) synthesized by AfeI, determined regulatory effects on genes expression, extracellular polymeric substance synthesis, energy...
This study evaluated the co-production of pigment and bacterial nanocellulose (BNC) from S. salsa biomass. The extraction beet red reduced salts flavonoids contents by 82.7%–100%, promoting efficiencies enzymatic saccharification biomass fermentation BNC hydrolysate. SEM analysis revealed that process disrupted lignocellulosic fiber structure, chemical lessened cellulase inhibitors, consequently facilitating for 10.4 times. producing strains were found to be hyper-sensitive NaCl stress,...
RpoS, one of the two alternative σ factors in Borrelia burgdorferi, is tightly controlled by multiple regulators and, turn, determines expression many critical virulence factors. Here we show that increasing RpoS causes cell death. The immediate effect was to promote bacterial division and as a consequence result rapid increase number before causing No DNA fragmentation or degradation observed during this induced Cryo-electron microscopy showed cells first formed blebs, which were eventually...
Acidithiobacillaceae, an important family of acidophilic and chemoautotrophic sulfur or iron oxidizers, participate in geobiochemical circulation the elements drive release heavy metals mining associated habitats. Because their environmental adaptability energy metabolic systems, Acidithiobacillus spp. have become dominant bacteria used bioleaching for metal recovery. Flagella-driven motility is with bacterial chemotaxis responses to stimuli. However, little known about how flagellum...
Acidithiobacillus spp. are prevalent in acid mine drainage, and they have been widely used biomining for extracting nonferrous metals from ores. The osmotic stress generated by elevated concentrations of inorganic ions is a severe challenge the growth bioleaching process; however, adaptation mechanism these bacteria to high pressure remains unclear. In this study, bioinformatics analysis indicated that response two-component system EnvZ-OmpR distributed spp., while OmpRs exhibited far more...
Studies have highlighted an upregulation of PD-1 expression in CD4
Acidithiobacillus sp. serves as biological factories for the synthesis of CdS quantum dots by directly using its bioleaching Cd ion from cadmium waste source.
Background Acidithiobacillus caldus, a Gram-negative, chemolithotrophic sulfur-oxidizing bacterium, is widely applied in bioleaching. The absence of an ideal selection marker has become major obstacle to achieve high efficiency the gene transfer system for A. caldus. Plasmid pJRD215, used spp., severe drawbacks molecular manipulations and potential biosafety issues due its mobility. Therefore, finding new constructing plasmids have urgent fundamental work Results Effective inhibitory effect...
Fruit juice industry generates a large amount of wastewater, which contains substantial waste activated carbon for turbidity removal. Due to the low concentrations useful compounds in cost extraction is extremely high. Two new bacterial nanocellulose (BNC) producing strains were isolated and identified. These produced 6.11 1.38 folds BNC from pear wastewater than conventional medium, respectively. spontaneously encapsulated suspended particles during fermentation, flocculating collecting...