A5072059728- Diet, Metabolism, and Disease
- Gut microbiota and health
- Biofuel production and bioconversion
- Diet and metabolism studies
- Microbial Metabolic Engineering and Bioproduction
- Pancreatic function and diabetes
- Phytochemicals and Antioxidant Activities
- Viral Infectious Diseases and Gene Expression in Insects
- Traditional Chinese Medicine Analysis
- Flavonoids in Medical Research
- Plant biochemistry and biosynthesis
- Amino Acid Enzymes and Metabolism
- Gastrointestinal motility and disorders
- Liver Disease Diagnosis and Treatment
- Enzyme Catalysis and Immobilization
- Gastroesophageal reflux and treatments
- Clostridium difficile and Clostridium perfringens research
- Advanced Control Systems Optimization
- Plant Gene Expression Analysis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Corrosion Behavior and Inhibition
- Drug Transport and Resistance Mechanisms
- Wastewater Treatment and Nitrogen Removal
- Video Coding and Compression Technologies
- Traditional Chinese Medicine Studies
Shanghai Jiao Tong University
2020-2025
Shandong Academy of Chinese Medicine
2016-2025
Ruijin Hospital
2022-2025
Renji Hospital
2020-2025
Suzhou Municipal Hospital
2025
Nanjing Medical University
2025
Shandong University
2023
National Cheng Kung University
2009
The gut microbiota interacts with intestinal epithelial cells through microbial metabolites to regulate the release of hormones. We investigated whether affects postprandial glucagon-like peptide-1 (GLP-1) response using antibiotic-treated mice and germ-free mice. Gut microbiome depletion completely abolished GLP-1 in circulation ileum a lipid tolerance test. Microbiome did not influence secretory function primary ileal stimulators vitro, but dramatically changed dynamics endogenous bile...
Calorie restriction plus dietary advice is suggested as a preventive strategy for individuals with obesity and prediabetes; however, optimal diet still debatable. We aimed to compare the effects of Mediterranean (MD) Chinese diets high or low in plants on body weight glucose homeostasis among high-risk Chinese.In this parallel-arm randomized controlled trial, 253 adults aged 25 60 years mass index ≥ 24.0 kg/m2 fasting blood 5.6 mmol/L were randomly assigned 3 isocaloric-restricted diets: MD...
Real-time localization and microbial activity information of indigenous gut microbiota over an extended period time remains a challenge with existing visualizing methods. Here, we report metabolic fluorine labeling (MEFLA)-based strategy for monitoring the dynamic via 19F magnetic resonance imaging (19F MRI). In situ different subgroups is achieved by using panel peptidoglycan-targeting MEFLA probes containing atoms chemical shifts, subsequent real-time in vivo accomplished multiplexed...
The gut microbiome is altered after bariatric surgery and associated with weight loss. However, the commensal bacteria involved underlying mechanism remain to be determined. We performed shotgun metagenomic sequencing in obese subjects before longitudinally sleeve gastrectomy (SG), found a significant enrichment microbial species Clostridia bile acid metabolizing genes SG treatment. Bile profiling further revealed decreased primary acids (PBAs) increased conjugated secondary (C-SBAs) SG....
Mapping the spatial locations of gut bacteria in their native environment enhances our understanding bacteria–host interactions and physiological pathological roles these microbes play. However, intricate composition bacterial communities millimeter-scale intestinal tissues presents a great challenge for situ imaging distributions. To address this, we introduce three-dimensional (3D) strategy that combines fluorescent tetrapeptide (TetraAA-AcLys) metabolic labeling probe with tissue clearing...
The translocation of bacteria from intestinal tracts into blood vessels and distal organs plays pivotal roles in the pathogenesis numerous severe diseases. Intravital monitoring bacterial translocation, however, is not yet feasible, which greatly hinders us comprehending this spatially temporally dynamic process. Here we report an vivo fluorogenic labeling method, enables situ imaging mouse gut microbiota real-time tracking translocated bacteria. By mimicking peptidoglycan stem peptide...
Abstract BACKGROUND D‐psicose is a rare sugar and exists in extremely small quantities nature. It has important physiological functions allowed to be used as an ingredient foods dietary supplements. The aim of this study develop the biotransformation, separation purification methods for highly efficient mass production d‐psicose. RESULTS First, gene d‐psicose 3‐epimerase (DPE) from Ruminococcus sp. was cloned overexpressed food‐grade microorganism Bacillus pumilus , recombinant protein...
As a malignant disease that seriously threatens human health, hepatocellular carcinoma (HCC) lacks effective early screening and prognostic assessment methods. Herein, we developed method for efficient capture multiphenotype analysis of circulating tumor cells (CTCs) carcinoma. The anti-ASGPR antibody the anti-EpCAM were modified in parallel on deterministic lateral displacement (DLD)-patterned microfluidic Synergetic-Chip to enhance efficiency by complementary effect. CTCs detected 45 out...
Bile acids (BAs) are closely related to nutrient supply and modified by gut microbiota. Gut microbiota perturbations shape BA composition, which further affects host metabolism.We investigated profiles in plasma, feces, liver of mice fed ad libitum, fasted for 24 h, h then refed using ultraperformance liquid chromatography coupled tandem mass spectrometry. was measured 16S rRNA gene sequencing. Expressions biosynthesis-related genes the reabsorption-related ileum were analyzed.Compared with...
Abstract BACKGROUND The rare sugar D‐psicose has important physiological functions, and been used as an ingredient in foods dietary supplements. It can be mass produced from D‐fructose industry. However, separation of is difficult. RESULTS In this research, a reaction purification system consisting two continuous stirred tank reactors (CSTR) containing immobilized glucose isomerase (GI) oxidase (GOD), respectively, for removal was constructed. Using transformed into gluconic acid separated...
By catalyzing a 3–3 cross-link in peptidoglycan, l,d-transpeptidases (Ldts) can cause resistance to β-lactams some pathogens vitro. However, the prevalence of Ldt and Ldt-mediated responses different vivo have never been explored. Here, we apply an metabolic labeling strategy study their biodistributions Ldt-induced bacterial mouse gut microbiota. A tetrapeptide-based fluorescent probe that functions as substrate for Ldts Gram-positive bacteria efficiently labels ∼18% total after gavage,...
The NAD(P)-dependent alcohol dehydrogenase (ADH) gene was cloned from Gluconobacter frateurii NBRC 3264 and expressed in Escherichia coli BL21 star (DE3). enzyme purified the characteristics were investigated. results showed that this ADH can convert allitol into D-allulose (D-psicose), which is first reported with catalytic ability. optimum temperature pH of 50°C 7.0, respectively, a maximal activity presence Co2+. At 1 mM Co2+ concentrations 50, 150, 250 mM, yields 97, 56, 38%, obtained...
Naolingsu capsule (NLSC) is a well-known traditional Chinese medicine (TCM) prescription in China. It widely used to treat neurasthenia, insomnia, cardiovascular and cerebrovascular disease, other diseases. However, its inalienable chemical groups have not been carried out. We first established the nontargeted investigation based on fingerprinting coupled with UHPLC-Q/TOF-MS/MS. Second, quantitative methods HPLC-DAD LC-MS/MS were connected synchronous assurance of eleven fourteen marker...
Bright flower colour assists plants attract insects to complete pollination and provides distinct ornamental values. In some medicinal plants, diverse variations usually imply differences in active ingredients. Compared the common bluish purple of Scutellaria baicalensis (SB), natural variants present rose red (SR) white (SW) flowers were screened out under same growing conditions genuine producing area Shandong Province, China. However, mechanism variation S. was remain unclear. study, we...