A5102771815- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Biochemical and Structural Characterization
- Bacillus and Francisella bacterial research
- Infective Endocarditis Diagnosis and Management
- Bacteriophages and microbial interactions
- Yersinia bacterium, plague, ectoparasites research
- Mollusks and Parasites Studies
- Antimicrobial Peptides and Activities
- Parasites and Host Interactions
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- Bacterial Identification and Susceptibility Testing
- Streptococcal Infections and Treatments
- Helminth infection and control
- Pharmacological Effects of Natural Compounds
- Microbial infections and disease research
- bioluminescence and chemiluminescence research
- Nosocomial Infections in ICU
- Machine Learning in Bioinformatics
- Escherichia coli research studies
- Autophagy in Disease and Therapy
- Microbial Metabolism and Applications
- Polysaccharides and Plant Cell Walls
Army Medical University
2014-2024
Second Xiangya Hospital of Central South University
2024
Central South University
2024
University of Malaya
2013
University of Alabama at Birmingham
2004-2013
University of Georgia
2012
The Ohio State University
2002
β-Lactam antibiotics are widely applied to treat infectious diseases. However, certain poor disease outcomes caused by β-lactams remain poorly understood. In this study, we have identified a cluster of lipoprotein-like genes ( lpl , sa2275 – sa2273 ) that is upregulated in the major clinically prevalent MRSA clones response subinhibitory concentrations β-lactam induction. The highlight work stimulate expression SarA, which directly binds promoter region and upregulates MRSA. Deletion...
ABSTRACT Moraxella osloensis , a gram-negative bacterium, is associated with Phasmarhabditis hermaphrodita nematode parasite of slugs. This bacterium-feeding has potential for the biological control slugs, especially grey garden slug, Deroceras reticulatum . Infective juveniles P. invade shell cavity develop into self-fertilizing hermaphrodites, and produce progeny, resulting in host death. However, role bacterium pathogenicity to slug unknown. We discovered that M. alone pathogenic D. after...
Vancomycin-intermediate Staphylococcus aureus (VISA) strains have spread globally. We previously isolated an ST239 VISA (XN108) with a vancomycin MIC of 12 mg/L. The mechanism for XN108 resistance to was investigated in this study.Genome comparison performed characterize mutations that might contribute the phenotype. novel mutation WalK(S221P) identified and using allelic replacement experiments. Vancomycin susceptibilities, autolytic activities morphologies were examined....
The accessory gene regulator (agr) quorum-sensing system is an important global regulatory of Staphylococcus aureus and contributes to its pathogenicity. S. agr divided into four groups based on the amino acid polymorphisms AgrB, AgrD, AgrC. activation group-specific, resulting in variations activity pathogenicity among groups. Strains with divergent always have different phenotypes. In present report, we, respectively, exchanged a certain other three alleles assessed corresponding...
Bubonic plague is transmitted by fleas whose feeding blocked a mass of Yersinia pestis in the digestive tract. Y. and closely related pseudotuberculosis also block Caenorhabditis elegans forming biofilm on nematode head. C. mutants with severe motility defects acquire almost no biofilm, indicating that normal animals accumulate matrix as they move through lawn. Using lectin wheat germ agglutinin probe, we show contains carbohydrate produced Yersinia. The present bacterial lawns prior to...
The detailed composition and structure of the Caenorhabditis elegans surface are unknown. Previous genetic studies used antibody or lectin binding to identify srf genes that play roles in determination. Infection by Microbacterium nematophilum identified bus (bacterially unswollen) also affect characteristics. We report biofilms produced Yersinia pestis Y. pseudotuberculosis, which bind C. predominantly on head, can be additional surface-determining genes. A screen for mutants with a biofilm...
The exosporium of Bacillus anthracis spores consists a basal layer and an external hair-like nap. nap is composed primarily the glycoprotein BclA, which contains collagen-like region with multiple copies pentasaccharide side chain. This oligosaccharide possesses unusual terminal sugar called anthrose, followed by three rhamnose residues protein-bound N-acetylgalactosamine. Based on structure we proposed enzymatic pathway for its biosynthesis. Examination B. genome revealed six contiguous...
Staphylococcus aureus is an important pathogen that produces abundant virulence factors, which cause various diseases burden human health worldwide. The stress response regulon called sigma factor B (SigB) a well-characterized global regulator involved in the regulation of S. virulence, pigmentation, and biofilm formation. However, regulatory network upon SigB incompletely described. Here, we identified novel substitution mutation, SigB(Q225P), contributed nonpigmented phenotype aureus....
Staphylococcus argenteus is a novel staphylococcal species (also considered as part of aureus complex) that infrequently reported on, and clinical S. infections are largely unstudied. Here, we report persistent recurrent hip joint infection case in which strain its small colony variants (SCVs) were successively isolated. We present features the two strains details their pathogenicity, explore factors induce SCVs formation course anti-infection therapy, reveal potential genetic mechanisms for...
Vancomycin-intermediate Staphylococcus aureus (VISA) is typically associated with a decline in virulence. We previously reported WalK(S221P) mutation that plays an important role mediating vancomycin resistance VISA XN108. Whether this implicated bacterial virulence remains unknown.This study aimed to investigate the effect of on and underlying mechanism effect.The influence its were explored using animal models, RNA-seq analysis, RT-qPCR, hemolytic assay, slide coagulase test, Western blot,...
ABSTRACT Moraxella osloensis is a gram-negative bacterium associated with Phasmarhabditis hermaphrodita , slug-parasitic nematode that has prospects for biological control of mollusk pests, especially the grey garden slug, Deroceras reticulatum . This bacterium-feeding acts as vector transports M. into shell cavity and killing agent in nematode-bacterium complex. We discovered produces an endotoxin(s), which tolerant to heat protease treatments kills slug after injection cavity. Washed or...
Bacillus anthracis spores are enclosed by an exosporium comprised of a basal layer and external hair-like nap. The filaments the nap composed trimers collagen-like glycoprotein BclA. attachment essentially all BclA to requires protein BxpB, both proteins included in stable high-molecular-mass complexes. contains proteolytically processed 38-residue amino-terminal domain (NTD) that is essential for basal-layer attachment. In this report, we identify three NTD submotifs (SM1a, SM1b, SM2,...
Staphylococcus aureus is the most common cause of difficult-to-treat osteomyelitis (OM). To better diagnose and manage S. OM, especially for severe long duration cases, indicators risk prediction severity evaluation are needed. Here, 139 clinical isolates from orthopedic infection were divided into OM group (60 60 patients) non-OM (79 79 patients). Molecular types, antimicrobial susceptibility, virulence factor profiles evaluated compared between two groups to identify potential associated...
Protein posttranslational modifications (PTMs) play important roles in regulating numerous biological functions of prokaryotic and eukaryotic organisms. Lysine succinylation (Ksucc) acetylation (Kac) are two PTMs that have been identified various bacterial species. However, the Ksucc Kac vancomycin-intermediate S. aureus (VISA) remain unclear. In this study, we systematically 3,260 sites 799 proteins 7,935 across 1,710 VISA strain XN108. Functional analyses revealed both were highly enriched...
Summary Bubonic plague is transmitted by fleas whose feeding blocked a Yersinia pestis biofilm in the digestive tract. Y. also block of Caenorhabditis elegans forming on nematode head, making an experimentally tractable surrogate for to study transmission. Arabinose 5‐phosphate isomerase (API), encoded yrbH, catalyses conversion ribulose into arabinose (A5P), first committed step 3‐deoxy‐ d ‐ manno ‐oct‐2‐ulosonic acid (Kdo) biosynthesis pathway. Here we show that YrbH multifunctional...
Bacterial brain abscesses (BAs) are difficult to treat with conventional antibiotics. Thus, the development of alternative therapeutic strategies for BAs is high priority. Identifying virulence determinants that contribute BA formation induced by Staphylococcus aureus would improve effectiveness interventions this disease. In study, RT-qPCR was performed compare expression levels 42 putative S. strains Newman and XQ during murine formation, ear colonization, bacteremia. The alterations in 23...
Abstract Staphylococcus aureus ( S. ), especially methicillin‐resistant (MRSA), causes wound infections, whose treatment remains a clinical challenge. Bacterium‐infected wounds often create acidic niches with pH 4.5–6.5. Endolysin LysSYL, which is derived from phage SYL, shows promise as an antistaphylococcal agent. However, endolysins generally exhibit instability and possess low bioavailability in microenvironments. Here, array of self‐assembling peptides designed, peptide L5 screened out...
Abstract Vancomycin (VAN)‐intermediate Staphylococcus aureus (VISA) is a critical cause of VAN treatment failure worldwide. Multiple genetic changes are reportedly associated with VISA formation, whereas strains often present common phenotypes, such as reduced autolysis and thickened cell wall. However, how mutated genes lead to phenotypes remains unclear. Here, we show metabolism regulatory cascade (CcpA‐GlmS), whereby two‐component systems (TCSs) link the VISA. We found that ccpA deletion...
The emergence of vancomycin-intermediate Staphylococcus aureus (VISA) has raised healthcare concerns worldwide. VISA is often associated with multiple genetic changes. However, the relative contributions these changes to phenotypes are incompletely defined. We have characterized XN108 vancomycin MIC 12 μg/ml. Genome comparison revealed that WalK(S221P), GraS(T136I), and RpoB(H481N) mutations possibly contributed phenotype XN108. In this study, above were stepwise cured, between its derivates...
Abstract Focal and systemic infections are serious threats to human health. Preclinical models enable the development of new drugs therapeutic regimens. In vivo, animal bioluminescence (BL) imaging has been used with bacterial reporter strains evaluate antimicrobial treatment effects. However, high‐sensitivity bioluminescent systems required because limited tissue penetration low brightness BL signals existing approaches. Here, we report that NanoLuc (Nluc) showed better performance than...
ABSTRACT The outermost exosporium layer of spores Bacillus anthracis , the causative agent anthrax, is comprised a basal and an external hairlike nap. nap includes filaments composed trimers collagenlike glycoprotein BclA. Essentially all BclA are tightly attached to spore in process requiring protein BxpB (also called ExsFA). Both incorporated into stable, high-molecular-mass complexes, suggesting that directly BxpB. 38-residue amino-terminal domain BclA, which normally proteolytically...