A5039545667- Ocular Surface and Contact Lens
- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Connexins and lens biology
- Intraocular Surgery and Lenses
- Glaucoma and retinal disorders
- Air Quality and Health Impacts
- Retinal Diseases and Treatments
- Allergic Rhinitis and Sensitization
- Ophthalmology and Visual Impairment Studies
- Cerebral Venous Sinus Thrombosis
- Pluripotent Stem Cells Research
- Ocular Diseases and Behçet’s Syndrome
- Advanced Glycation End Products research
- Glioma Diagnosis and Treatment
- Healthcare Systems and Public Health
- Cancer-related molecular mechanisms research
- Cerebrospinal fluid and hydrocephalus
- Travel-related health issues
- Cancer Immunotherapy and Biomarkers
- Intraoperative Neuromonitoring and Anesthetic Effects
- Cardiovascular Health and Risk Factors
- Silk-based biomaterials and applications
- Vagus Nerve Stimulation Research
- Climate Change and Health Impacts
Second Affiliated Hospital of Zhejiang University
2016-2024
Zhejiang University
2015-2021
National Natural Science Foundation of China
2016-2020
North China University of Science and Technology
2020
Zhejiang Center for Disease Control and Prevention
2016
Zhejiang Chinese Medical University
2016
Zhejiang Provincial Hospital of TCM
2016
Creative Commons
2016
Affiliated Eye Hospital of Wenzhou Medical College
2016
Lanzhou University Second Hospital
2016
Purpose: The pathological mechanisms underlying cataract formation remain largely unknown on account of the lack appropriate in vitro cellular models. aim this study is to develop a stable system for human lens regeneration using pluripotent stem cells. Methods: Isolated urinary cells were infected with four Yamanaka factors generate induced (UiPSCs), which differentiate into progenitor and lentoid bodies (LBs). expression lens-specific markers was examined by real-time PCR, immunostaining,...
Purpose: To compare the visual outcomes and optical quality of 2 presbyopia-correcting intraocular lenses (IOLs) with those a monofocal IOL. Settings: Eye Center, Second Affiliated Hospital Zhejiang University, School Medicine, Hangzhou, Zhejiang, China. Design: Prospective cohort study. Methods: The study included patients who had cataract surgery were implanted Tecnis Symfony Extended Range Vision (EROV) IOL (ZXR00), zonal refractive multifocal (Lentis Comfort LS-313 MF15), or L-313)....
The aging of lens progenitor cell has been repeatedly proposed to play a key role in age-related cataracts (ARCs), but the mechanism is far from being understood. present study aims investigate relationship between progenitor/epithelial cells and 4 subtypes ARCs humans.Lens capsules, which were collected ARC patients during surgery, divided into 3 groups according age (50-60, 60-80, >80 years). expressions cell-related markers Sox2, Abcg2, Ki67 first examined human epithelial (HLECs) situ....
Abstract The scarcity of corneal tissue to treat deep defects and perforations remains a challenge. Currently, small incision lenticule extraction (SMILE)-derived lenticules appear be promising alternative for the treatment these conditions. However, thickness toughness single piece are limited. To overcome limitations, we constructed stromal equivalent with SMILE-derived fibrin glue. In vitro cell culture revealed that could provide suitable scaffold survival proliferation epithelial cells,...
Abstract We assessed the effectiveness of light-guided-tip intense pulsed light (IPL) with meibomian gland expression (MGX) in chalazion treatment. Ninety-five eyes received a IPL-MGX treatment (IPL-MGX group), and another 95 incision curettage (Control group). Prior to IPL or incision, as well 1 month after final treatment, data were gathered pertaining lesion location size, hyperemia, lesions regression recurrence, comprehensive ophthalmic examination. The total size chalazia group was...
Despite the recent breakthrough in cataract drug development, further improvements have been limited by lack of human vitro disease models. This study, therefore, aims to generate a qualified model. Mature lentoid bodies (LBs) on Day 25 (D25), which were differentiated from induced pluripotent stem cells (iPSCs) using "fried egg" method, continually culturing (control) or extra treated with either ultraviolet (UV) radiation hydrogen peroxide (H2 O2 ). The LBs' shape alteration and opacity...
Nonsteroidal anti-inflammatory drugs (NSAIDs) have shown antifibrotic effects on several diseases. The aims of the present in vitro study were to investigate bromfenac (a kind NSAID) primary human pterygium fibroblasts (HPFs) and conjunctival (HConFs), as well explore possible mechanisms these effects.The cells used this HPFs HConFs, profibrotic activation was induced by transforming growth factor-beta1 (TGF-β1). Western blot, quantitative real-time PCR, immunofluorescence (IF) assays detect...
Purpose. It has been confirmed that inflammatory cytokines are involved in the progression of pterygium. Histamine can enhance proliferation and migration many cells. Therefore, we intend to investigate proliferative migratory effects histamine on primary culture human pterygium fibroblasts (HPFs). Methods. Pterygium conjunctiva samples were obtained from surgery, toluidine blue staining was used identify mast 3-[4, 5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) performed...
Abstract Congenital cataracts are the leading cause of childhood blindness. To date, surgical removal is only established treatment, but surgery associated with multiple complications, which often lead to visual impairment. Therefore, mechanistic studies and drug-candidate screening have been intrigued by aims developing novel therapeutic strategies. However, these hampered a lack an appropriate human-disease model congenital cataracts. Herein, we report establishment human cataract in vitro...
Autophagy is essential in lens organelle degradation. This study aimed to seek potential autophagy-associated long noncoding RNAs (lncRNAs) and their relative mechanisms human development using the "fried egg" lentoid body (LB) generation system. The expression pattern of LC3B differentiating LBs was similar that developing a mouse vivo. Among massive lncRNAs expressed with significant difference between induced pluripotent stem cells (iPSCs) LBs, lncRNA affecting (ALB), which predicted have...
The exact pathogenesis of idiopathic choroidal neovascularization (ICNV) remains unclear. Cytokine-mediated inflammation has been thought to be involved in the pathophysiology ICNV. purpose this study was investigate serum cytokine profiles patients with ICNV and explore relationship between levels severity. This case-control conducted 32 30 healthy volunteers. Clinical demographic information obtained from medical data platform analysed a multiplex assay determine seven cytokines:...
Our previous study observed that human induced pluripotent stem cell (HiPSC)-derived lentoid bodies (LBs) became cloudy with extended culture time, partially mimicking the progress of age-related cataracts (ARCs) in a dish. In present study, lanosterol, potential anticataract drug, was used to further verify value this model drug screening for cataract treatment.
Objectives This study aimed to assess the effectiveness and safety of intense pulsed light (IPL) therapy plus topical 0.05% cyclosporine A (CsA) eye drops treat Sjögren's Syndrome-related dry eyes (SS-DE).
This study aimed to determine the therapeutic effectiveness of different machines in intense pulsed light (IPL) treatment meibomian gland dysfunction (MGD).