A5103110248- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Tendon Structure and Treatment
- Osteoarthritis Treatment and Mechanisms
- Mesenchymal stem cell research
- Knee injuries and reconstruction techniques
- RNA Interference and Gene Delivery
- Animal Genetics and Reproduction
- Silk-based biomaterials and applications
- Periodontal Regeneration and Treatments
- Epigenetics and DNA Methylation
- Zebrafish Biomedical Research Applications
- Renal and related cancers
- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Nuts composition and effects
- Genomics and Chromatin Dynamics
- Advanced biosensing and bioanalysis techniques
- Bone Tissue Engineering Materials
- Corneal Surgery and Treatments
- Telomeres, Telomerase, and Senescence
- Biochemical and Molecular Research
- Connexins and lens biology
- Cancer-related gene regulation
- Fatty Acid Research and Health
Zhejiang University
2014-2024
Second Affiliated Hospital of Zhejiang University
2024
First Affiliated Hospital Zhejiang University
2020-2023
Hangzhou First People's Hospital
2023
Wenzhou Medical University
2023
Affiliated Eye Hospital of Wenzhou Medical College
2023
Nanjing General Hospital of Nanjing Military Command
2014-2022
Southern Medical University
2022
Nanjing University
2022
Universitas Prima Indonesia
2021
Mutations in human induced pluripotent stem cells (iPSCs) pose a risk for their clinical use due to preferential reprogramming of mutated founder cell and selection mutations during maintenance iPSCs culture. It is unknown, however, if are stress associated with oncogene expression reprogramming. We performed whole exome sequencing foreskin fibroblasts derived at two different passages. found that vitro passaging contributed 7% the iPSC coding point mutation load, ultradeep amplicon revealed...
As tendon stem/progenitor cells were reported to be rare in tissues, tendons as vulnerable targets of sports injury possess poor self-repair capability. Human ESCs (hESCs) represent a promising approach regeneration. But their teno-lineage differentiation strategy has yet defined. Here, we report that force combined with the tendon-specific transcription factor scleraxis synergistically promoted commitment hESCs tenocyte for functional tissue Force and can independently induce...
Abstract Meniscus injury is frequently encountered in clinical practice. Current surgical therapy involving partial or complete meniscectomy relieves pain the short-term but often leads to osteoarthritis (OA) long-term. In this study, we report a new strategy of articular cartilage protection by intra-articular injection novel human meniscus stem/progenitor cells (hMeSPCs). We found that hMeSPCs displayed both mesenchymal stem cell characteristics and high expression levels collagen II. rat...
Degenerate eye disorders, such as glaucoma, cataracts and age-related macular degeneration (AMD), are prevalent causes of blindness visual impairment worldwide. Other including limbal stem cell deficiency (LSCD), dry diseases (DED), retinitis pigmentosa (RP), result in symptoms ocular discomfort impaired function, significantly impacting quality life. Traditional therapies limited, primarily focus on delaying disease progression, while emerging therapy directly targets tissues, aiming to...
Highlights•During reprogramming, oxidative stress is elevated•Antioxidants reduce genome instability during reprogramming•iPSCs made in the presence of antioxidants have fewer de novo genomic aberrationsSummarySomatic cells can be reprogrammed to induced pluripotent stem (iPSCs) using oncogenic transcription factors. However, this method leads genetic aberrations iPSCs via unknown mechanisms, which may limit their clinical use. Here, we demonstrate that supplementation growth media with...
Despite our previous study that demonstrates human embryonic stem cells (hESCs) can be used as seed for tendon tissue engineering after stepwise induction, suboptimal regeneration implies a new strategy needs to developed repair. We investigated whether overexpression of the tendon-specific transcription factor scleraxis (SCX) in hESC-derived mesenchymal (hESC-MSCs) together with knitted silk-collagen sponge scaffold could promote regeneration.hESCs were initially differentiated into MSCs...
The first cellular differentiation event in mouse development leads to the formation of blastocyst consisting inner cell mass (ICM) and trophectoderm (TE). transcription factor CDX2 is required for proper TE specification, where it promotes expression genes, represses Pou5f1 (OCT4). However its downstream network developing embryo not fully characterized. Here, we performed high-throughput single qPCR analysis Cdx2 null embryos identify CDX2-regulated targets vivo. To genes likely be...
Purpose: The pathological mechanisms underlying cataract formation remain largely unknown on account of the lack appropriate in vitro cellular models. aim this study is to develop a stable system for human lens regeneration using pluripotent stem cells. Methods: Isolated urinary cells were infected with four Yamanaka factors generate induced (UiPSCs), which differentiate into progenitor and lentoid bodies (LBs). expression lens-specific markers was examined by real-time PCR, immunostaining,...
Meniscus injury is frequently encountered in clinical practice. Current surgical therapy involving partial or complete meniscectomy relieves pain the short-term but often leads to osteoarthritis (OA) long-term. Here, this study aimed identify and characterize a novel population of meniscus-derived stem cells (MeSCs) develop new strategy articular cartilage protection by intra-articular injection these cells. The "stemness" immune properties MeSCs were investigated vitro, while efficacy for...
Methionine metabolism is critical for the maintenance of embryonic stem cells (ESCs) and induced pluripotent (iPSCs) pluripotency. However, little known about regulation methionine cycle to sustain ESC Here, we show that adenosylhomocysteinase (AHCY), an important enzyme in cycle, differentiation mouse (mESCs). We mESCs exhibit high levels metabolism, whereas decreasing via depletion AHCY promotes differentiate into three germ layers. posttranslationally modified with O-linked...
Abstract The cellular mechanism and target cell affected by stromal microenvironments in augmenting hematopoietic specification from pluripotent human embryonic stem cells (hESCs) has yet to be evaluated. Here, contrast aorta-gonad-mesonephros-derived S62 cells, OP9 inhibit apoptosis also augment the proliferation of hemogenic precursors prospectively isolated embryoid bodies. In addition, stroma supported within primitive compartment inhibiting CD45+CD34+ committed lineage, but have no...
Mechanisms that govern hematopoietic lineage specification, as opposed to the expansion of committed progenitors, from human pluripotent stem cells (hPSCs) have yet be fully defined. Here, we show within family genes called inhibitors differentiation (ID), ID1 and ID3 negatively regulate transition lineage-specified hemogenic progenitors during hematopoiesis both embryonic (hESCs) induced cell (hiPSCs). Upon induction hPSCs, levels transcripts rapidly increase, peaking at stage precursor...
Immunological response hampers the investigation of human embryonic stem cells (hESCs) or their derivates for tissue regeneration in vivo. Immunosuppression is often used after surgery, but exhibits side effects significant weight loss and allows only short-term observation. The purpose this study was to investigate whether neonatal desensitization supports relative long-term survival hESC-derived mesenchymal (hESC-MSCs) promotes cartilage regeneration. hESC-MSCs were injected on day birth...
The GGC repeat expansion in the NOTCH2NLC gene has been identified as genetic cause of neuronal intranuclear inclusion disease (NIID). Recently, this was also reported to be associated with essential tremor (ET). However, some patients expansion, initially diagnosed ET, were eventually NIID. Therefore, controversy remains regarding clinical diagnosis these expansion-positive presenting tremor-dominant symptoms. This study aimed clarify phenotype who have gene.We screened for pathogenic...
The conversion of somatic cells into pluripotent stem via overexpression reprogramming factors involves epigenetic remodeling. DNA methylation at a significant proportion CpG sites in induced (iPSCs) differs from that embryonic (ESCs). Whether different sets influence the type and extent aberrant iPSCs differently remains unknown. In order to help resolve this critical question, we generated human common fibroblast cell source using either Yamanaka (OCT4, SOX2, KLF4 cMYC) or Thomson NANOG...