A5063624341- Tendon Structure and Treatment
- Knee injuries and reconstruction techniques
- Shoulder Injury and Treatment
- Silk-based biomaterials and applications
- Cellular Mechanics and Interactions
- Sports injuries and prevention
- Tissue Engineering and Regenerative Medicine
- Laser Applications in Dentistry and Medicine
- Single-cell and spatial transcriptomics
- Electrospun Nanofibers in Biomedical Applications
- Mesenchymal stem cell research
- Osteoarthritis Treatment and Mechanisms
- Heterotopic Ossification and Related Conditions
- Periodontal Regeneration and Treatments
- 3D Printing in Biomedical Research
- CAR-T cell therapy research
- Wound Healing and Treatments
- Bone Tissue Engineering Materials
- Extracellular vesicles in disease
- Plant Surface Properties and Treatments
- Muscle Physiology and Disorders
- Orthopedic Surgery and Rehabilitation
- Metabolism and Genetic Disorders
- Biomedical and Engineering Education
- CRISPR and Genetic Engineering
Zhejiang University
2016-2025
Sir Run Run Shaw Hospital
2020-2025
Zhejiang Lab
2025
Second Affiliated Hospital of Zhejiang University
2018-2024
Zhejiang University-University of Edinburgh Institute
2018-2024
Wuyi University
2022-2024
North China University of Science and Technology
2024
Hangzhou Red Cross Hospital
2016-2021
StemCells (United States)
2021
Chinese University of Hong Kong
2016-2018
Abstract Human embryonic stem cells (hESCs) are ideal seed for tissue regeneration, but no research has yet been reported concerning their potential tendon regeneration. This study investigated the strategy and efficacy of using hESCs regeneration as well mechanism involved. were first induced to differentiate into mesenchymal (MSCs), which had three lineages positive MSC surface markers. hESC-derived MSCs (hESC–MSCs) regenerated tissues in both an vitro engineering model vivo ectopic model,...
Abstract Articular cartilage is not a physiologically self-renewing tissue. Injury of often progresses from the articular surface to subchondral bone, leading pathogenesis tissue degenerative diseases, such as osteoarthritis. Therapies treat defects using autologous chondrocyte-based engineering have been developed and used for more than 20 years; however, challenge chondrocyte expansion in vitro remains. A promising cell source, stem/progenitor cells (CSPCs), has attracted recent attention....
Abstract The transcription factor Mohawk (Mkx) is expressed in developing tendons and an important regulator of tenogenic differentiation. However, the exact roles Mkx tendinopathy tendon repair remain unclear. Using gene expression Omnibus datasets immunofluorescence assays, we found that level was dramatically lower human tissue it activated at specific stages development. In mesenchymal stem cells (MSCs), ectopic strikingly promoted tenogenesis more efficiently than Scleraxis (Scx), a...
Tendon stem/progenitor cells (TSPCs) were recently identified within tendon tissues. The aim of this study was to investigate TSPC-seeded knitted silk—collagen sponge scaffold for functional shoulder repair. multidifferentiation potential, proliferation, and immune properties TSPCs investigated in vitro, while the efficacy scaffolds promoting rotator cuff regeneration evaluated vivo a rabbit model. TSPCs, which exhibited universal stem cell characteristics (i.e., clonogenicity, high...
Nestin defines a novel tendon stem/progenitor cell subpopulation with high tenogenesis potential.
As tendon stem/progenitor cells were reported to be rare in tissues, tendons as vulnerable targets of sports injury possess poor self-repair capability. Human ESCs (hESCs) represent a promising approach regeneration. But their teno-lineage differentiation strategy has yet defined. Here, we report that force combined with the tendon-specific transcription factor scleraxis synergistically promoted commitment hESCs tenocyte for functional tissue Force and can independently induce...
Background: Chronic tendinopathy is a commonly occurring clinical problem that affects both athletes and inactive middle-aged patients. Although some studies have shown different platelet-rich plasma (PRP) preparations could exert various therapeutic effects in vitro, the role of leukocytes PRP has not yet been defined under conditions vivo. Purpose: This study compared intratendon delivery leukocyte-poor (Lp-PRP) versus leukocyte-rich (Lr-PRP) rabbit chronic model Study Design: Controlled...
Abstract Meniscus injury is frequently encountered in clinical practice. Current surgical therapy involving partial or complete meniscectomy relieves pain the short-term but often leads to osteoarthritis (OA) long-term. In this study, we report a new strategy of articular cartilage protection by intra-articular injection novel human meniscus stem/progenitor cells (hMeSPCs). We found that hMeSPCs displayed both mesenchymal stem cell characteristics and high expression levels collagen II. rat...
Abstract Tendon injuries are common and present a clinical challenge, as they often respond poorly to treatment result in long-term functional impairment. Inferior tendon healing responses mainly attributed insufficient or failed tenogenesis. The main objective of this study was establish an efficient approach induce tenogenesis bone marrow-derived mesenchymal stem cells (BMSCs), which the most seed tissue engineering. First, representative reported tenogenic growth factors were used media...
Heterotopic ossification (HO) is pathological bone formation characterized by within muscle, tendons, or other soft tissues. However, the cells of origin and mechanisms involved in pathogenesis HO remain elusive. Here we show that deletion suppressor fused (Sufu) cathepsin K–Cre–expressing (Ctsk-Cre–expressing) resulted spontaneous progressive ligament, tendon, periarticular ossification. Lineage tracing studies cell functional analysis demonstrated Ctsk-Cre could label a subpopulation...