A5028040749- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Nonlinear Photonic Systems
- COVID-19 Clinical Research Studies
- Advanced Fiber Laser Technologies
- SARS-CoV-2 and COVID-19 Research
- Nonlinear Waves and Solitons
- Neonatal Respiratory Health Research
- Long-Term Effects of COVID-19
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Asthma and respiratory diseases
- Nonlinear Dynamics and Pattern Formation
- Cancer, Hypoxia, and Metabolism
- Protein Structure and Dynamics
- COVID-19 and healthcare impacts
- Medical Imaging and Pathology Studies
- Occupational and environmental lung diseases
- Respiratory Support and Mechanisms
- RNA modifications and cancer
- Pediatric health and respiratory diseases
- Epigenetics and DNA Methylation
- Pulmonary Hypertension Research and Treatments
- Advanced NMR Techniques and Applications
- Peroxisome Proliferator-Activated Receptors
- Advanced Nanomaterials in Catalysis
- Cancer, Lipids, and Metabolism
Huazhong University of Science and Technology
2014-2024
Tongji Hospital
2014-2024
People's Hospital of Xinjiang Uygur Autonomous Region
2021-2024
October 6 University
2024
Zhejiang University
2024
Ningbo University
2024
State Development & Investment Corporation (China)
2024
Cangzhou Central Hospital
2023
Army Medical University
2017-2023
Fuzhou University
2022-2023
BACKGROUNDThe coronavirus disease 2019 (COVID-19), infected by severe acute respiratory syndrome 2 (SARS-CoV-2), has caused a outbreak throughout the world. The host immunity of COVID-19 patients is unknown.METHODSThe routine laboratory tests and in with different severity illness were compared after patient admission.RESULTSA total 65 SARS-CoV-2-positive classified as having mild (n = 30), 20), extremely 15) illness. Many tests, such ferritin, lactate dehydrogenase, D-dimer, increased...
In December 2019, an outbreak of the severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2) infection occurred in Wuhan, and rapidly spread to worldwide, which has attracted many people's concerns about patients. However, studies on status medical personnel is still lacking. A total 54 cases SARS-Cov-2 infected staff from Tongji Hospital between 7 January 11 February 2020 were analyzed this retrospective study. Clinical epidemiological characteristics compared different groups by...
Despite past extensive studies, the mechanisms underlying pulmonary fibrosis (PF) still remain poorly understood. Here, we demonstrated that lungs originating from different types of patients with PF, including coronavirus disease 2019, systemic sclerosis-associated interstitial lung disease, and idiopathic mice following bleomycin (BLM)-induced PF are characterized by altered methyl-CpG-binding domain 2 (MBD2) expression in macrophages. Depletion Mbd2 macrophages protected against...
Abstract SARS-CoV-2 induced marked lymphopenia in severe patients with COVID-19. However, whether lymphocytes are targets of viral infection is yet to be determined, although RNA or antigen has been identified T cells from patients. Here, we confirmed that could detected patient peripheral blood (PBCs) postmortem lung cells, and the infectious virus also antigen-positive PBCs. We next prove infects lymphocytes, preferably activated CD4 + vitro. Upon infection, RNA, subgenomic protein...
Current treatments for idiopathic pulmonary fibrosis slow the rate of lung function decline, but may be associated with adverse events that affect medication adherence. In phase 2 trials, pamrevlumab (a fully human monoclonal antibody binds to and inhibits connective tissue growth factor activity) attenuated progression without substantial events.
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Abstract Objectives Hypoxia is an important risk factor for pulmonary arterial remodelling in hypertension (PAH), and the Janus kinase 2 (JAK2) believed to be involved this process. In present report, we aimed investigate role of JAK2 vascular smooth muscle cells during course PAH. Methods Smooth cell (SMC)‐specific Jak2 deficient mice their littermate controls were subjected normobaric normoxic or hypoxic (10% O ) challenges 28 days monitor development PAH, respectively. To further...
Although DNA methylation has been recognised in the pathogenesis of idiopathic pulmonary fibrosis (IPF), exact mechanisms are yet to be fully addressed. Herein, we demonstrate that lungs originated from IPF patients and mice after bleomycin (BLM)-induced characterised by altered along with overexpression myofibroblasts methyl-CpG-binding domain 2 (MBD2), a reader responsible for interpreting methylome-encoded information. Specifically, depletion Mbd2 fibroblasts or protected BLM-induced...
Significance The mechanisms of how Cyclophilin A (CypA) regulates HIV-1 infectivity remain poorly understood. We examined the role dynamics in capsid (CA) protein assemblies by magic-angle-spinning NMR. assembled CA is highly dynamic. Dipolar tensors calculated from molecular trajectories are quantitative agreement with NMR results. Motions CypA loop sequence-dependent and attenuated escape mutants A92E G94D. Dynamics similar CA/CypA complex. These findings suggest that escapes dependence...
Airway eosinophilic inflammation is a key feature of type 2 high asthma. The role epithelial microRNA (miR) in airway remains unclear. We examined the expression miR-221-3p bronchial brushings, induced sputum, and plasma from 77 symptomatic, recently diagnosed, steroid-naive subjects with asthma 36 healthy controls by quantitative PCR analyzed correlation between eosinophilia. found that epithelial, was significantly decreased Epithelial correlated eosinophil sputum biopsies, fraction...
Since the outbreak of 2019 novel coronavirus (SARS-CoV-2) pneumonia, many patients with underlying disease, such as interstitial lung disease (ILD), were admitted to Tongji hospital in Wuhan, China. To date, no data have ever been reported reflect clinical features Corona Virus Disease (COVID-19) among these preexisting ILD. We analyzed incidence and severity COVID-19 ILD 3201 inpatients, compared two independent cohorts pre-existing (n = 28) non-ILD 130). Among those 28 whom (0.88%). Fever...
Since the outbreak of 2019 novel coronavirus (severe acute respiratory syndrome 2 [SARS-CoV-2]) pneumonia, thousands patients with fever or cough were flocked into clinic designated hospitals in Wuhan, China. To date, no data have ever been reported to reflect prevalence disease (COVID-19) among these outpatients. Moreover, it is almost unknown discriminate COVID-19 and nucleic acid negative based on clinical features clinics.The infectious status SARS-CoV-2 was estimated The epidemiological...
Abstract Kdm2a catalyzes H3K36me2 demethylation to play an intriguing epigenetic regulatory role in cell proliferation, differentiation, and apoptosis. Herein we found that myeloid-specific knockout of ( LysM- Cre- f/f , −/− ) promoted macrophage M2 program by reprograming metabolic homeostasis through enhancing fatty acid uptake lipolysis. increased levels at the Pparg locus along with augmented chromatin accessibility Stat6 recruitment, which rendered macrophages preferential polarization....
Maturation of HIV-1 particles encompasses a complex morphological transformation Gag via an orchestrated series proteolytic cleavage events. A longstanding question concerns the structure C-terminal region CA and peptide SP1 (CA-SP1), which represents intermediate during maturation virus. By integrating NMR, cryo-EM, molecular dynamics simulations, we show that in CA-SP1 tubes assembled vitro, represent features assembly state maturation, exists dynamic helix-coil equilibrium, addition...
Abstract Type 1 diabetes (T1D) is characterized by the selective autoimmune destruction of islet β cells, and macrophages play a significant role in this process. Small ubiquitin-like modification (SUMOylation) an important posttranslational involved T1D pathogenesis, but its function remains unexplored. We presently developed used macrophage-specific ubiquitin-conjugating enzyme E2 ( Ubc9 ) knockout (LyzM-Cre -Ubc9 fl/fl , KO) mice to address impact SUMOylation on macrophage model. observed...
HIV-1 CA capsid protein possesses intrinsic conformational flexibility, which is essential for its assembly into conical capsids and interactions with host factors. dynamic in the assembled capsid, residues functionally important regions of undergo motions spanning many decades time scales. Chemical shift anisotropy (CSA) tensors, recorded magic-angle-spinning NMR experiments, provide direct residue-specific probes on nano- to microsecond We combined NMR, MD, density-functional-theory...
We report dynamic nuclear polarization (DNP)-enhanced magic-angle spinning (MAS) NMR spectroscopy in viral capsids from HIV-1 and bacteriophage AP205. Viruses regulate their life cycles infectivity through modulation of structures dynamics. While static several viruses are now accessible with near-atomic-level resolution, atomic-level understanding functionally important motions assembled is lacking. observed up to 64-fold signal enhancements by DNP, which permitted in-depth analysis these...