A5032502576- Osteoarthritis Treatment and Mechanisms
- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- Mesenchymal stem cell research
- Tendon Structure and Treatment
- Sirtuins and Resveratrol in Medicine
- Inflammatory mediators and NSAID effects
- Growth Hormone and Insulin-like Growth Factors
- Knee injuries and reconstruction techniques
- Bone Metabolism and Diseases
- Metabolomics and Mass Spectrometry Studies
- Cell Adhesion Molecules Research
- RNA Interference and Gene Delivery
- Periodontal Regeneration and Treatments
- Silk-based biomaterials and applications
- TGF-β signaling in diseases
- Wnt/β-catenin signaling in development and cancer
- Metabolism and Genetic Disorders
- Exercise and Physiological Responses
- Tissue Engineering and Regenerative Medicine
- Peroxisome Proliferator-Activated Receptors
- Calpain Protease Function and Regulation
- Prosthetics and Rehabilitation Robotics
- Proteoglycans and glycosaminoglycans research
- Cancer, Hypoxia, and Metabolism
Ohio University
2020-2024
Johns Hopkins University
1989-2021
Oklahoma Medical Research Foundation
2017-2021
Johns Hopkins Medicine
2018-2021
Zhejiang University
2013-2020
Zhejiang University-University of Edinburgh Institute
2020
Second Affiliated Hospital of Zhejiang University
2020
Xiangya Hospital Central South University
2020
Central South University
2020
Soil and Fertilizer Institute of Hunan Province
2020
Joint pain is the defining symptom of osteoarthritis (OA) but its origin and mechanisms remain unclear. Here, we investigated an unprecedented role osteoclast-initiated subchondral bone remodeling in sensory innervation for OA pain. We show that osteoclasts secrete netrin-1 to induce nerve axonal growth bone. Reduction osteoclast formation by knockout receptor activator nuclear factor kappa-B ligand (Rankl) osteocytes inhibited nerves into bone, dorsal root ganglion neuron hyperexcitability,...
Whether sensory nerve can sense bone density or metabolic activity to control homeostasis is unknown. Here we found prostaglandin E2 (PGE2) secreted by osteoblastic cells activates PGE2 receptor 4 (EP4) in nerves regulate formation inhibiting sympathetic through the central nervous system. osteoblasts increases when decreases as demonstrated osteoporotic animal models. Ablation of erodes skeletal integrity. Specifically, knockout EP4 gene cyclooxygenase-2 (COX2) significantly reduces volume...
Our incomplete understanding of osteoarthritis (OA) pathogenesis has significantly hindered the development disease-modifying therapy. The functional relationship between subchondral bone (SB) and articular cartilage (AC) is unclear. Here, we found that changes SB architecture altered distribution mechanical stress on AC. Importantly, latter well aligned with pattern transforming growth factor beta (TGFβ) activity in AC, which essential regulation AC homeostasis. Specifically, TGFβ...
Abstract The transcription factor Mohawk (Mkx) is expressed in developing tendons and an important regulator of tenogenic differentiation. However, the exact roles Mkx tendinopathy tendon repair remain unclear. Using gene expression Omnibus datasets immunofluorescence assays, we found that level was dramatically lower human tissue it activated at specific stages development. In mesenchymal stem cells (MSCs), ectopic strikingly promoted tenogenesis more efficiently than Scleraxis (Scx), a...
The periosteum, a thin tissue that covers almost the entire bone surface, accounts for more than 80% of human mass and is essential regeneration. Its osteogenic regenerative abilities are well studied, but much unknown about periosteum. In this study, we found macrophage-lineage cells recruit periosteum-derived (PDCs) cortical formation. Knockout colony stimulating factor-1 eliminated resulted in loss PDCs with impaired periosteal Moreover, TRAP+ induced transcriptional expression periostin...
Background: Osteoarthritis is the leading cause of disability worldwide; cartilage degeneration and defects are central features. Significant progress in tissue engineering holds promise to regenerate damaged tissue. However, a formidable challenge develop 3-dimensional (3D) construct that can regulate local immune environment facilitate intrinsic osteochondral regeneration. Purpose: To evaluate efficacy 3D-printed decellularized extracellular matrix (ECM) polyethylene glycol diacrylate...
Aortic compliance in normotensive and hypertensive Chinese subjects undergoing diagnostic cardiac catheterization was compared by using a newly described method that allows for determination of the pressure dependence if one assumes value exponential coefficient pressure-volume relation large arteries. Under baseline conditions groups at mean aortic pressures 96.3 128.6 mm Hg, averaged 1.47 0.80 ml/mm respectively. Compliance group diastolic 99.4 Hg (which nearly equal to pressure) 1.072...
Because cartilage and bone tissues have different lineage‐specific biological properties, it is challenging to fabricate a single type of scaffold that can biologically fulfill the requirements for regeneration these two lineages simultaneously within osteochondral defects. To overcome this challenge, lithium‐containing mesoporous bioglass (Li‐MBG) developed. The efficacy mechanism Li‐MBG defects are systematically investigated. Histological micro‐CT results show scaffolds significantly...
Epigenetic mechanisms have been reported to play key roles in chondrogenesis and osteoarthritis (OA) development. Here, we sought identify specific histone demethylases that are involved delineate the underlying mechanisms.We screened expression of 17 distinct by quantitative real time PCR (qRT-PCR) during chondrogenic differentiation C3H10T1/2 cells. The role Kdm6b cartilage development was then analysed with transgenic Col2a1-CreERT2;Kdm6bf/f . RNA-Seq applied explore changes chondrocytes...
Background Osteoarthritis (OA) is a degenerative joint disease characterised by cartilage degradation and chondrocyte hypertrophy. A recent study showed that Rac1 promoted expression of MMP13 hypertrophy within the growth plate. These findings warrant further investigations on roles in OA development therapy animal models. Objective To investigate role mechanistic pathway involvement pathological changes chondrocytes vitro vivo, as well to develop strategy modulating activity for treatment....
Osteoarthritis (OA) remains an intractable clinical challenge. Few drugs are available for reversing this degenerative disease, although some promising candidates have performed well in preclinical studies. Tumor necrosis factor α (TNFα) has been identified as a crucial effector modulating OA pathogenesis. This study aimed to investigate the therapeutic effects of Atsttrin, novel TNFα blocker, on treatment. We developed genetically modified mesenchymal stem cells (MSCs) that expressed...
ABSTRACT Understanding how obesity-induced metabolic stress contributes to synovial joint tissue damage is difficult because of the complex role metabolism in development, maintenance, and repair. Chondrocyte mitochondrial dysfunction implicated osteoarthritis (OA) pathology, which motivated us study deacetylase enzyme sirtuin 3 (Sirt3). We hypothesized that combining high-fat-diet (HFD)-induced obesity cartilage Sirt3 loss at a young age would impair chondrocyte function, leading cellular...
Excessive growth hormone (GH) has been shown to promote joint degeneration in both preclinical and clinical studies. Little is known about the effect of disrupted GH or receptor (GHR) on health. The goal this study investigate pathology mice with either germline (GHR
Chondrocyte hypertrophy and mineralization are considered to be important pathologic factors in osteoarthritis (OA). We previously reported that Rac1 was aberrantly activated promote chondrocyte hypertrophy, mineralization, expression of matrix metalloproteinase 13 ADAMTS OA. However, the underlying mechanism aberrant activation OA is unclear. The present study undertaken identify specific molecular regulator controlling activity OA, as well investigate its function development.Expression...
ABSTRACT Epigenetic regulation is highly correlated with osteoarthritis (OA) development, whereas its role and detailed mechanisms remain elusive. In this study, we explored the expression of EZH2, an H3K27me3 transferase, in human OA cartilages roles regulating pathogenesis. Here, found EZH2 was expressed both mice cartilage samples by using histological analysis RNA sequencing (RNA-Seq). The medial meniscectomy (MMx) model results indicated conditional knockout Ezh2 deteriorated...
Objective Obesity accelerates the development of osteoarthritis (OA) during aging and is associated with altered chondrocyte cellular metabolism. Protein lysine malonylation (MaK) a posttranslational modification (PTM) that has been shown to play an important role obesity. The objective this study was investigate sirtuin 5 (Sirt5) in regulating MaK metabolism chondrocytes under obesity-related conditions. Methods SIRT5 were immunostained knee articular cartilage obese db/db mice different...