A5078160004- Osteoarthritis Treatment and Mechanisms
- Spine and Intervertebral Disc Pathology
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- interferon and immune responses
- Inflammatory mediators and NSAID effects
- Bone Tissue Engineering Materials
- Spinal Hematomas and Complications
- Autophagy in Disease and Therapy
- Pulmonary Hypertension Research and Treatments
- Spinal Cord Injury Research
- Inflammasome and immune disorders
- Natural product bioactivities and synthesis
- Cytokine Signaling Pathways and Interactions
- Knee injuries and reconstruction techniques
- MicroRNA in disease regulation
- Medical Imaging and Pathology Studies
- Mesenchymal stem cell research
- Circular RNAs in diseases
- Orthopaedic implants and arthroplasty
- Immune Response and Inflammation
- Kawasaki Disease and Coronary Complications
- Total Knee Arthroplasty Outcomes
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
Zhejiang University
2016-2024
Zhejiang Lab
2023-2024
First Affiliated Hospital Zhejiang University
2018-2024
Second Affiliated Hospital of Zhejiang University
2020-2024
Blood Center of Zhejiang Province
2024
Brigham and Women's Hospital
2024
Harvard University
2024
Zhejiang University Medical College Affiliated Stomatological Hospital
2024
Massachusetts Institute of Technology
2024
Zhejiang University-University of Edinburgh Institute
2018-2020
Abstract Intervertebral disc degeneration (IDD) is a complicated process that involves both cellular apoptosis and senescence. Metformin has been reported to stimulate autophagy, whereas autophagy shown protect against Therefore, we hypothesize metformin may have therapeutic effect on IDD through stimulation. The of was investigated in vitro vivo . Our study showed attenuated senescence induced by tert-butyl hydroperoxide nucleus pulposus cells. Autophagy, as well its upstream regulator...
Abstract Damaged deoxyribonucleic acid (DNA) is a primary pathologic factor for osteoarthritis (OA); however, the mechanism by which DNA damage drives OA unclear. Previous research demonstrated that cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) participates in response. As result, current study aimed at exploring role STING, major effector cGAS-STING signaling casacde, progress vitro, as well vivo. In this study, expression STING was evaluated human and mouse tissues,...
Multilayer scaffolds fabricated by 3D printing or other techniques have been used to repair osteochondral defects. However, it remains a challenge regenerate the articular cartilage and subchondral bone simultaneously with higher performance. In present study, we enhanced efficiency of defects developing bi-layer scaffold: an interleukin-4 (IL-4)-loaded radially oriented gelatin methacrylate (GelMA) scaffold printed digital light processing (DLP) in upper layer porous polycaprolactone...
Abstract The limited molecular classifications and disease signatures of osteoarthritis (OA) impede the development prediagnosis targeted therapeutics for OA patients. To classify understand subtypes OA, we collected three types tissue including cartilage, subchondral bone, synovium from multiple clinical centers constructed an extensive transcriptome atlas By applying unsupervised clustering analysis to cartilage transcriptome, patients were classified into four with distinct signatures: a...
The blockage of autophagic flux in chondrocytes has been considered as a major reason for the excessive cellular apoptosis and senescence osteoarthritis (OA) development; however, molecular mechanism therapeutic strategy interrupted is still not clear. Most recently, transcription factor EB (TFEB) identified master regulator via initiating expression multiple autophagy-related genes lysosomal biogenesis. This research was performed to confirm whether TFEB activity are impacted OA development...
Objective The aim of this study was to evaluate and compare three common nutritional screening tools with the new European Society for Clinical Nutrition Metabolism (ESPEN) diagnostic criteria malnutrition among elderly patients gastrointestinal cancer. Research methodsandprocedures Nutritional tools, including Risk Screening 2002 (NRS 2002), Malnutrition Universal Tool (MUST) Short Form Mini Assessment (MNA-SF), were applied 255 We compared values these malnutrition, using ESPEN as ‘gold...
Epigenetic mechanisms have been reported to play key roles in chondrogenesis and osteoarthritis (OA) development. Here, we sought identify specific histone demethylases that are involved delineate the underlying mechanisms.We screened expression of 17 distinct by quantitative real time PCR (qRT-PCR) during chondrogenic differentiation C3H10T1/2 cells. The role Kdm6b cartilage development was then analysed with transgenic Col2a1-CreERT2;Kdm6bf/f . RNA-Seq applied explore changes chondrocytes...
Rheumatoid arthritis (RA) is a common autoimmune disease leading to pain, disability, and even death. Although studies have revealed that aberrant activation of STING was implicated in various diseases, the role RA remains unclear. In current study, we demonstrated pivotal pathogenesis. As accumulation dsDNA, specific stimulus for STING, feature RA, developed spherical polyethyleneimine-coated mesoporous polydopamine nanoparticles loaded with antagonist C-176 (PEI-PDA@C-176 NPs) treating RA....
Osteoarthritis (OA) is one of the most common degenerative joint diseases and associated with autophagy suppression. However, molecular mechanism regulation in context OA not fully understood. In this study, we sought to determine role that HECTD1 plays pathogenesis OA.We used RNA sequencing analysis explore differential expression E3 ubiquitin ligase genes healthy human cartilage affected by OA. Using surgery- aging-induced mouse models, comprehensively analyzed function screened gene...
Abstract Background Extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) offer promising prospects for stimulating cartilage regeneration. The different formation mechanisms suggest that exosomes and ectosomes possess biological functions. However, little attention has been paid to the differential effects of EV subsets on Methods Our study compared two EVs isolated adipose-derived MSCs (ASCs) chondrocytes bone marrow–derived (BMSCs) in vitro. Additionally, we loaded...
Lumbar disc degeneration is a common cause of chronic low back pain and an important contributor to various degenerative lumbar spinal disorders. However, currently there no effective therapeutic strategy for treating degeneration. The pro-inflammatory cytokine interleukin-1β (IL-1β) mediates by inducing apoptotic death nucleus pulposus (NP) cells degradation the NP extracellular matrix. Here, we confirmed that secretion IL-1β via secretory autophagy contributes degeneration, demonstrate...
Osteoarthritis (OA), a degenerative disorder, is considered to be one of the most common forms arthritis. Limonin (Lim) extracted from lemons and other citrus fruits. has been reported have anti-inflammatory effects, while inflammation major cause OA; thus, we propose that limonin may therapeutic effect on OA. In this study, OA was assessed in chondrocytes vitro IL-1β induced destabilization medial meniscus (DMM) mice vivo. The Nrf2/HO-1/NF-κB signaling pathway evaluated illustrate working...
Nanoliposomes have a broad range of applications in the treatment autoimmune inflammatory diseases because their ability to considerably enhance drug transport. For clinical application, nanoliposomes must be able realize on-demand release drugs at disease sites maximize drug-delivery efficacy and minimize side effects. Therefore, responsive drug-release strategies for inflammation been explored; however, no specific design has realized system based on pyroptosis-related inflammation....
Small-interfering RNAs (siRNAs) offer promising prospects for treating pyroptosis-related autoimmune diseases. However, poor stability and off-target effects during in vivo transportation hinder their practical clinical applications. Precision delivery adaptive release of siRNAs into inflamed tissues immune cells could unleash full therapeutic potential. This study establishes a pyroptotic-spatiotemporally selective siRNA system (PMRC@siGSDME) that selectively targets inflammatory tissues,...
Akebia Saponin D promotes the translocation of NRF2 into nucleus, activates NRF2/HO-1 pathway and inhibits NF-κB in chondrocytes, ultimately alleviates osteoarthritis development.
Abstract Objectives Diabetes is a risk factor for intervertebral disc degeneration (IVDD). Studies have demonstrated that diabetes may affect IVDD through transcriptional regulation; however, whether post‐transcriptional regulation involved in diabetic (DB‐IVDD) still unknown. This study was performed to illustrate the role of HuR, an RNA‐binding protein, DB‐IVDD development and its mechanism. Materials Methods The expression HuR evaluated nucleus pulposus (NP) tissues from patients high...
ABSTRACT Epigenetic regulation is highly correlated with osteoarthritis (OA) development, whereas its role and detailed mechanisms remain elusive. In this study, we explored the expression of EZH2, an H3K27me3 transferase, in human OA cartilages roles regulating pathogenesis. Here, found EZH2 was expressed both mice cartilage samples by using histological analysis RNA sequencing (RNA-Seq). The medial meniscectomy (MMx) model results indicated conditional knockout Ezh2 deteriorated...