A5049082258- Spine and Intervertebral Disc Pathology
- Osteoarthritis Treatment and Mechanisms
- Natural product bioactivities and synthesis
- NF-κB Signaling Pathways
- Inflammatory mediators and NSAID effects
- Toxoplasma gondii Research Studies
- interferon and immune responses
- Clinical practice guidelines implementation
- Spinal Hematomas and Complications
- Orthopaedic implants and arthroplasty
- Autophagy in Disease and Therapy
- Bone fractures and treatments
- Inflammasome and immune disorders
- Phytochemical compounds biological activities
- Berberine and alkaloids research
- Elbow and Forearm Trauma Treatment
- Bone Metabolism and Diseases
- Cervical and Thoracic Myelopathy
- Signaling Pathways in Disease
- Cytokine Signaling Pathways and Interactions
- Hip disorders and treatments
- Pharmacological Effects of Medicinal Plants
- RNA Interference and Gene Delivery
- Chemokine receptors and signaling
- Neuroinflammation and Neurodegeneration Mechanisms
Wenzhou Medical University
2015-2025
Second Affiliated Hospital & Yuying Children's Hospital of Wenzhou Medical University
2018-2025
Abstract The pathophysiology of spinal cord injury (SCI) involves primary and secondary injury. Secondary is a major target for SCI therapy, whereas microglia play an important role in immunoresponsive gene 1 (Irg-1) has been recorded as one the most significantly upregulated genes tissues chip data; however, its remains unclear. This study aims to illustrate Irg-1 well regulated metabolite itaconate SCI. It was demonstrated that expression increased mice stimulated by lipopolysaccharides...
The purpose of our study was to elucidate the role histone methyltransferase enhancer zeste homologue 2 (EZH2) in pathophysiology osteoarthritis (OA) and develop a strategy modulate EZH2 activity for OA treatment. expression normal human cartilage compared by western blotting. effect overexpression inhibition on chondrocyte hypertrophy related gene examined real-time PCR, methylation promoter Wnt inhibitor SFRP1 analyzed using chromatin immunoprecipitation (ChIP) PCR. Histological assessment...
Abstract Osteoarthritis (OA) is a complicated joint disorder characterized by inflammation that causes destruction. Cucurbitacin B (CuB) naturally occurring triterpenoid compound derived from plants in the Cucurbitaceae family. The aim of this study to investigate potential role and mechanisms CuB mouse model OA. This identified key targets pathways through network pharmacology analysis. In vivo vitro studies confirmed Through pharmacology, 54 for treating OA were identified. therapeutic...
Cartilaginous endplate (CEP) degeneration is considered as one of the major causes intervertebral disc (IVDD) which low back pain. Recent studies have proved that epigenetic alteration involved in a variety diseases. This work explored role histone methyltransferase enhancer zeste homologue 2 (EZH2) CEP degeneration, well its underlying mechanisms, and confirmed effect EZH2 knockdown on delaying IVDD development.Western blotting, immunofluorescence staining, ChIP assay were applied to...
Osteoarthritis (OA), a degenerative disorder, is considered to be one of the most common forms arthritis. Limonin (Lim) extracted from lemons and other citrus fruits. has been reported have anti-inflammatory effects, while inflammation major cause OA; thus, we propose that limonin may therapeutic effect on OA. In this study, OA was assessed in chondrocytes vitro IL-1β induced destabilization medial meniscus (DMM) mice vivo. The Nrf2/HO-1/NF-κB signaling pathway evaluated illustrate working...
Osteoarthritis (OA) is a chronic and degenerative condition that persists progresses over time. Sipeimine (Sip), steroidal alkaloid derived from Fritillariae Cirrhosae Bulbus, has attracted considerable attention due to its exceptional anti-inflammatory, analgesic, antioxidant, anti-cancer characteristics. However, Sip's effects on OA mechanism still need further research. This study utilized network pharmacology identify initial targets for Sip. Functional associations of Sip in were...
Osteoarthritis (OA) is a prevalent degenerative disease that lacks effective therapy. Oxidative stress one of the major factors contributing to OA; however, treatments targeting oxidative are still lacking. In current study, we established an stress-induced cell death model in chondrocytes vitro and screened drugs may suppress death. Ethyl gallate (EG) was identified as most potent drug against out more than 600 natural product library. Application without appropriate delivery system for OA...
Abstract Exosomes derived from bone marrow mesenchymal stem cells (BMSCs) hold significant potential in the treatment of osteoarthritis (OA). However, simple exosomes therapy often proves insufficiently effective and challenging to achieve optimal therapeutic outcome. Herein, an innovative injectable multifunctional composite hydrogel (Exo shEZH2 @HG) is composed photocrosslinking partially modified hyaluronic acid with o ‐nitrobenzyl alcohol (HA‐NB), gelatin, shRNA‐EZH2 endogenously ),...
Diabetic osteoporosis is a complication of diabetes mellitus, and can result in an increased incidence bone fractures delay fracture healing. Berberine one the most widely distributed isoquinoline alkaloid plants possesses antioxidant properties. These properties reduce high glucose mediated dysfunction human marrow stem cells. Therefore, present study was designed to investigate apparent beneficial effect berberine on characteristics streptozotocin plus HFD-induced diabetic rats. Rats were...
Abstract Objectives Diabetes is a risk factor for intervertebral disc degeneration (IVDD). Studies have demonstrated that diabetes may affect IVDD through transcriptional regulation; however, whether post‐transcriptional regulation involved in diabetic (DB‐IVDD) still unknown. This study was performed to illustrate the role of HuR, an RNA‐binding protein, DB‐IVDD development and its mechanism. Materials Methods The expression HuR evaluated nucleus pulposus (NP) tissues from patients high...
ABSTRACT Intervertebral disc degeneration (IVDD) is a complex process involving many factors, among which excessive senescence of nucleus pulposus cells considered to be the main factor. Our previous study found that metformin can inhibit in cells; however, mechanism such an action was still largely unknown. In current study, we inactivates cGAS-STING pathway during oxidative stress. Furthermore, knockdown STING (also known as STING1) suppresses senescence, indicating might exert its effect...
ABSTRACT The intervertebral disk (IVD) is avascular and anaerobic glycolysis has been recognized as the main source of energy. Due to glycolysis, there are high levels lactate production in disk. Previous study shows concentration elevated degenerated IVD. However, it not clear how causes degeneration disks. In this study, we found that 2 mM promote proliferation NP cells, while 6 slightly inhibit their proliferation. By detection under transmission electron microscopy, western bolt for...
Abstract Diabetes (DB) is a risk factor for osteoarthritis progression. High glucose (HG) one of the key pathological features DB and has been demonstrated to induce apoptosis senescence in chondrocytes. Autophagy an endogenous mechanism that can protect cells against senescence. The effects HG on autophagy including chondrocytes have studied; however, results inconsistent. current study aimed elucidate underlying mechanisms, which could be associated with contrasting outcomes. present...
Osteoarthritis (OA) is a common degenerative joint disease featuring the degeneration, destruction, and ossification of cartilage. Inflammation which may facilitate OA occurrence development considered as main pathological factor. Betulin, natural product extracted from birch bark, has been commonly used for inflammation treatment; however, its role in remains unclear. This study aimed to explore whether betulin can suppress IL-1β-induced chondrocytes alleviate vitro vivo. In studies,...
IL-1β promotes inflammatory response and extracellular matrix (ECM) degradation through NF-κB signaling pathway; while cardamonin attenuates the ECM by suppressing pathway<italic>via</italic>Nrf2/HO-1 axis.
β-HIVS blocks the activity of NF-κB by activating Nrf2/HO-1 pathway, thereby inhibiting IL-1β-induced inflammation and ECM degradation.
In this study, we used murine chondrocytes as an in vitro model and mice exhibiting destabilization of the medial meniscus (DMM) vivo to investigate mechanisms through which S-allyl cysteine (SAC) alleviates osteoarthritis (OA). SAC significantly reduced apoptosis senescence maintained homeostasis extracellular matrix (ECM) metabolism tert-butyl hydroperoxide (TBHP)-treated chondrocytes. Molecular docking analysis showed a -CDOCKER interaction energy value 203.76 kcal/mol for interactions...
Intervertebral disc degeneration (IVDD) has been reported to be a major cause of low back pain. Studies have demonstrated that IVDD may dysregulated at the transcriptional level; however, whether post-transcriptional regulation is involved still unknown. The current study aimed illustrate role Human antigen R (HuR), an RNA binding protein in regulation, IVDD. results showed expression HuR was decreased degenerative nucleus pulposus (NP) tissues as well TNF-α-treated NP cells. Downregulation...
The treatment of senile distal radius fractures had not been clearly defined. objective this study was to identify the factors associated with decision for operative displaced in patients aged over 55 years.Data 318 were collected on patient-, fracture- and surgeon-related characteristics that plausibly related operation. Mean comparisons or chi-squared test used univariate analysis above-mentioned factors, then multiple logistic regression operation.Univariate showed age, osteoporosis,...