A5075065209- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Cardiovascular Function and Risk Factors
- Metabolomics and Mass Spectrometry Studies
- Metabolism, Diabetes, and Cancer
- Diet and metabolism studies
- Osteoarthritis Treatment and Mechanisms
- Muscle Physiology and Disorders
- Analytical chemistry methods development
- Analytical Chemistry and Chromatography
- Redox biology and oxidative stress
- Trace Elements in Health
- Endoplasmic Reticulum Stress and Disease
- Sulfur Compounds in Biology
- Retinal Diseases and Treatments
- Antioxidant Activity and Oxidative Stress
- Biochemical Acid Research Studies
- Peroxisome Proliferator-Activated Receptors
- Nitric Oxide and Endothelin Effects
- Cancer, Hypoxia, and Metabolism
- Metabolism and Genetic Disorders
- Fatty Acid Research and Health
- Exercise and Physiological Responses
Oklahoma Medical Research Foundation
2015-2024
University of Oklahoma Health Sciences Center
2012-2023
Cleveland Clinic
2002-2013
Cleveland Clinic Lerner College of Medicine
2001-2013
University of Oklahoma Medical Center
2012
Central South University
2008
University School
2002-2008
Case Western Reserve University
2002-2008
Aarhus University
2008
Cerner (United States)
2007
In recent studies we demonstrated that systemic levels of protein-bound nitrotyrosine (NO2Tyr) and myeloperoxidase (MPO), a protein catalyzes generation nitrating oxidants, serve as independent predictors atherosclerotic risk, burden, incident cardiac events. We now show both apolipoprotein A-I (apoA-I), the primary constituent HDL, is selective target for MPO-catalyzed nitration chlorination in vivo oxidation HDL apoA-I results inhibition ABCA1-dependent cholesterol efflux from macrophages....
In recent studies we demonstrated that systemic levels of protein-bound nitrotyrosine (NO2Tyr) and myeloperoxidase (MPO), a protein catalyzes generation nitrating oxidants, serve as independent predictors atherosclerotic risk, burden, incident cardiac events. We now show both apolipoprotein A-I (apoA-I), the primary constituent HDL, is selective target for MPO-catalyzed nitration chlorination in vivo oxidation HDL apoA-I results inhibition ABCA1-dependent cholesterol efflux from macrophages....
Chronic hyperglycemia causes structural alterations of proteins through the Maillard reaction. In diabetes, methylglyoxal (MGO)-induced hydroimidazolones are predominant modification. contrast to acute hyperglycemia, mitochondrial respiration is depressed in chronic diabetes. To determine whether MGO-derived protein modifications result abnormalities bioenergetics and superoxide formation, proteomics functional studies were performed renal cortical mitochondria isolated from rats with 2, 6,...
Summary Sarcopenia, the loss of skeletal muscle mass and function during aging, is a major contributor to disability frailty in elderly. Previous studies found protective effect reduced histone deacetylase activity models neurogenic atrophy. Because aging associated with motor neuron innervation, we investigated potential for ( HDAC ) inhibitor butyrate modulate age‐related loss. Consistent previous studies, significant hindlimb 26‐month‐old C57Bl/6 female mice fed control diet. Butyrate...
Adjustment of cerebral blood flow (CBF) to neuronal activity via neurovascular coupling (NVC) has an essential role in maintenance healthy cognitive function. In aging increased oxidative stress and cerebromicrovascular endothelial dysfunction impair NVC, contributing decline. There is increasing evidence showing that a decrease NAD+ availability with age plays critical range age-related cellular impairments but its impaired NVC responses remains unexplored. The present study was designed...
The C-to-U editing of apolipoprotein B (apo-B) mRNA is catalyzed by a multiprotein complex that recognizes an 11-nucleotide mooring sequence downstream the site. catalytic subunit enzyme, apobec-1, has cytidine deaminase activity but requires additional unidentified proteins to edit apo-B mRNA. We purified 65-kDa protein functionally complements apobec-1 and obtained peptide information which was used in molecular cloning experiments. complementation factor (ACF) cDNA encodes novel 64.3-kDa...
We recently reported that apolipoprotein A-I (apoA-I), the major protein component of high density lipoprotein, is a selective target for myeloperoxidase (MPO)-catalyzed nitration and chlorination in both serum subjects with cardiovascular disease. further showed extent apoA-I correlated functional impairment reverse cholesterol transport activity isolated lipoprotein. Herein we used tandem mass spectrometry to map sites MPO-mediated vitro vivo relate degree site-specific modifications loss...
Abstract Reactive oxygen species and reactive nitrogen produced by epithelial inflammatory cells are key mediators of the chronic airway inflammation asthma. Detection 3-nitrotyrosine in asthmatic lung confirms presence increased species, but lack identification modified proteins has hindered an understanding potential mechanistic contributions nitration/oxidation to inflammation. In this study, we applied a proteomic approach, using nitrotyrosine as marker, evaluate oxidation...
Obesity is a predictor of diabetes and cardiovascular disease. One consequence obesity dyslipidemia characterized by high blood triglycerides. It has been proposed that oxidative stress, driven utilization lipids for energy, contributes to these diseases. The effects stress are mitigated an endogenous antioxidant enzyme network, but little known about its response fat utilization. Our experiments used multiplexed quantitative proteomics method measure expression in heart tissue mouse model...
Obesity has deleterious effects on cognitive function in the elderly adults. In mice, aging exacerbates obesity-induced oxidative stress, microvascular dysfunction, blood–brain barrier (BBB) disruption, and neuroinflammation, which compromise health. However, specific mechanisms through obesity interact to remain elusive. Previously, we have shown that Nrf2 signaling plays a critical role resilience is associated with progressive promoting impairment. To test hypothesis deficiency...
A decline in mitochondrial function and biogenesis as well increased reactive oxygen species (ROS) are important determinants of aging. With advancing age, there is a concomitant reduction circulating levels insulin-like growth factor-1 (IGF-1) that closely associated with neuronal aging neurodegeneration. In this study, we investigated the effect IGF-1 signaling age on astrocyte metabolism its association learning memory.Learning memory was assessed using radial arm water maze young old...
Mutations in polycystin-1 (PC1) lead to autosomal-dominant polycystic kidney disease (ADPKD), a leading cause of renal failure for which no treatment is available. PC1 an integral membrane protein, has been implicated the regulation multiple signaling pathways including JAK/STAT pathway. Here we show that membrane-anchored activates STAT3 JAK2-dependent manner, tyrosine phosphorylation and transcriptional activity. The C-terminal cytoplasmic tail can undergo proteolytic cleavage nuclear...
Objective To quantify functional age‐related changes in the cartilage antioxidant network order to discover novel mediators of oxidative stress and osteoarthritis (OA) pathophysiology. Methods We evaluated histopathologic knee OA 10‐, 20‐, 30‐month‐old male F344BN rats analyzed oxidation according ratio reduced oxidized glutathione. Antioxidant gene expression protein abundance were by quantitative reverse transcription–polymerase chain reaction selected reaction–monitoring mass...
Mice deficient in the antioxidant enzyme Cu/Zn-superoxide dismutase (Sod1-/- or Sod1KO mice) have increased oxidative stress, show accelerated aging and develop spontaneous hepatocellular carcinoma (HCC) with age. Similar to humans, HCC development mice progresses from non-alcoholic fatty liver disease (NAFLD) steatohepatitis (NASH) fibrosis, which eventually HCC. Oxidative stress plays a role NAFLD NASH progression, inflammation is main mechanism that drives progression fibrosis. Because...
Isolates of Pseudomonas aeruginosa from chronic lung infections in cystic fibrosis (CF) patients have phenotypes distinct those initially infecting CF patients, as well other clinical or environmental isolates. To gain a better understanding the differences these isolates, protein expression was followed using two-dimensional (2-D) gel electrophoresis and identification by peptide sequencing micro-capillary column liquid chromatography–tandem mass spectrometry (μLC/MS/MS). The isolates...
Protein tyrosine phosphorylation has been associated with both capacitation and motility of mammalian sperm. During capacitation, human spermatozoa undergo a characteristic set proteins, only one which thus far cloned localized. We report here the sequence fibrous sheath protein 95 kDa (FSP95) that undergoes during similarity to sperm A-kinase anchor proteins (AKAPs). FSP95 is auto- iso-antigenic in humans as it recognized by sera containing antisperm antibodies from infertile men women. The...