A5080142354- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Genetics and Neurodevelopmental Disorders
- Identity, Memory, and Therapy
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer-related gene regulation
- Neurotransmitter Receptor Influence on Behavior
- Retinal Diseases and Treatments
- Retinal Development and Disorders
- Mitochondrial Function and Pathology
- Pancreatic function and diabetes
- Immune Response and Inflammation
- Cellular transport and secretion
- Birth, Development, and Health
- Memory and Neural Mechanisms
- Adipose Tissue and Metabolism
- Retinal Imaging and Analysis
- Photosynthetic Processes and Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- ATP Synthase and ATPases Research
- Retinopathy of Prematurity Studies
- Neuroscience and Neuropharmacology Research
- Cancer Cells and Metastasis
- Neurological Disease Mechanisms and Treatments
- GDF15 and Related Biomarkers
University of Oklahoma Health Sciences Center
2014-2023
Star Center
2017-2019
Czech Academy of Sciences, Institute of Physiology
2019
Swift Engineering (United States)
2019
University of Oklahoma
2014-2017
Oklahoma City University
2017
Pennsylvania State University
2012-2016
Penn State Milton S. Hershey Medical Center
2014
OU Health
2014
The necessity of including both males and females in molecular neuroscience research is now well understood. However, there relatively limited basic biological data on brain sex differences across the lifespan despite age-related neurological dysfunction disease between females. Whole genome gene expression young (3 months), adult (12 old (24 months) male female C57BL6 mice hippocampus was analyzed. Subsequent bioinformatic analyses confirmations changes hippocampal protein were performed....
The growing interest in the role of epigenetic modifications human health and disease has led to development next-generation sequencing methods for whole genome analysis DNA methylation patterns. However, many projects require targeted specific genes or genomic regions. We have developed an approach, termed BiSulfite Amplicon Sequencing (BSAS), hypothesis driven focused absolute analysis. This approach is applicable both studies as well confirmation genome-wide studies.BSAS uses PCR...
A decline in mitochondrial function and biogenesis as well increased reactive oxygen species (ROS) are important determinants of aging. With advancing age, there is a concomitant reduction circulating levels insulin-like growth factor-1 (IGF-1) that closely associated with neuronal aging neurodegeneration. In this study, we investigated the effect IGF-1 signaling age on astrocyte metabolism its association learning memory.Learning memory was assessed using radial arm water maze young old...
Abstract The genomic hypomethylation hypothesis of aging proposes that an overall decrease in global DNA methylation occurs with age, and it has been argued the could be important factor aging, resulting relaxation gene expression regulation abnormal expression. Since was initially observed decreased age 1974, 16 articles have published describing effect on various tissues from rodents humans. We critically reviewed publications enzymes involved to evaluate validity aging. On basis current...
The role of epigenetic processes in the control gene expression has been known for a number years. DNA methylation at cytosine residues is particular interest studies as it demonstrated to be both long lasting and dynamic regulator expression. Efforts examine changes health disease have hindered by lack high-throughput, quantitatively accurate methods. With advent popularization next-generation sequencing (NGS) technologies, these tools are now being applied epigenomics addition existing...
Summary DNA methylation is a central regulator of genome function, and altered patterns are indicative biological aging mortality. Age‐related cellular, biochemical, molecular changes in the hippocampus lead to cognitive impairments greater vulnerability neurodegenerative disease that varies between sexes. The role hippocampal epigenomic with these processes unknown as no genome‐wide analyses age‐related have considered factor sex controlled animal model. High‐depth, bisulfite sequencing...
The major histocompatibility complex I (MHCI) pathway, which canonically functions in innate immune viral antigen presentation and detection, is functionally pleiotropic the central nervous system (CNS). Alternative roles include developmental synapse pruning, regulation of synaptic plasticity, inhibition neuronal insulin signaling; all processes altered during brain aging. Upregulation MHCI components with aging has been reported; however, no systematic examination cellular localization,...
Changes to the epigenome with aging, and DNA modifications in particular, have been proposed as a central regulator of aging process, predictor mortality, contributor pathogenesis age-related diseases. In nervous system, control learning memory, neurogenesis, plasticity require changes cytosine methylation hydroxymethylation. Although genome-wide decreases are often reported scientific dogma, primary research reports describe decreases, increases, or lack change hydroxymethylation their...
Impairment of hippocampal-dependent spatial learning and memory with aging affects a large segment the aged population. Hippocampal subregions (CA1, CA3, DG) have been previously reported to express both common specific morphological, functional, gene/protein alterations cognitive decline. To comprehensively assess gene expression decline, transcriptomic analysis CA1, DG was conducted using Adult (12M) Aged (26M) F344xBN rats behaviorally characterized by Morris water maze performance. Each...
The role of epigenetic processes in the control gene expression has been known for a number years. DNA methylation at cytosine residues is particular interest studies as it demonstrated to be both long lasting and dynamic regulator expression. Efforts examine changes health disease have hindered by lack high-throughput, quantitatively accurate methods. With advent popularization next-generation sequencing (NGS) technologies, these tools are now being applied epigenomics addition existing...
Diabetic retinopathy is a neurovascular diabetes complication resulting in vision loss. A wealth of literature reports retinal molecular changes indicative neural deficits, inflammation, and vascular leakage with chronic diabetes, but the mechanistic causes disease initiation progression are unknown. Microvascular mitochondrial DNA (mtDNA) damage leading to dysfunction has been proposed drive retinopathy. However, growing evidence suggests that retina precedes may cause damage. Therefore, we...
Abstract Background Alterations to cellular and molecular programs with brain aging result in cognitive impairment susceptibility neurodegenerative disease. Changes DNA methylation patterns, an epigenetic modification required for various CNS functions are observed can be prevented by anti-aging interventions, but the relationship of altered gene expression is poorly understood. Results Paired analysis hippocampal methylome transcriptome male female mice demonstrates that age-related...
Summary DNA methylation is a central regulator of genome function and altered patterns are indicative biological aging mortality. Age-related cellular, biochemical, molecular changes in the hippocampus lead to cognitive impairments greater vulnerability neurodegenerative disease that varies between sexes. The role hippocampal epigenomic with these processes unknown as no genome-wide analyses age-related have considered factor sex controlled animal model. High-depth, bisulfite sequencing...
Abstract Brain aging is marked by cognitive decline and susceptibility to neurodegeneration. Caloric-restriction (CR) increases neurogenesis, improves memory function, protects from age-associated neurological disorders. Epigenetic mechanisms, including DNA methylation, are vital normal CNS cellular functions, dysregulated with aging. The beneficial effects of CR have been proposed work through epigenetic processes, but this largely unexplored. We therefore tested whether life-long prevents...
Abstract Normalase is a novel enzymatic library normalization method that eliminates quantification and manual concentration adjustment of each sample prior to pooling, the uniform processing numerous error-prone pipetting steps. When combined with Swift 2S Turbo rapid kits, highly streamlined ‘Turbolase’ workflow created readily automated on Hamilton Star other platforms, where simple bulk improves throughput reduces cost for NGS laboratories. kits comprise two steps single purification...