A5101898086- Plant Molecular Biology Research
- Genetic Mapping and Diversity in Plants and Animals
- Plant Genetic and Mutation Studies
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Bioenergy crop production and management
- Plant Reproductive Biology
- Wheat and Barley Genetics and Pathology
- Plant Virus Research Studies
- Chromosomal and Genetic Variations
- Light effects on plants
- Genetic and phenotypic traits in livestock
- Natural product bioactivities and synthesis
- Plant responses to elevated CO2
- Advanced Nanomaterials in Catalysis
- Plant Pathogens and Fungal Diseases
- Plant Parasitism and Resistance
- Hydrogen embrittlement and corrosion behaviors in metals
- Biofuel production and bioconversion
- Plant responses to water stress
- Mycotoxins in Agriculture and Food
- Genomics and Phylogenetic Studies
- Corrosion Behavior and Inhibition
- Plant Surface Properties and Treatments
Cropping Systems Research Laboratory
2015-2024
Agricultural Research Service
2013-2023
Henan Normal University
2021-2023
United States Department of Agriculture
2012-2022
Henan Academy of Agricultural Sciences
2021-2022
Shanghai Normal University
2020-2021
United States Department of State
2015
Xian Yang Central Hospital
2011-2012
Dean McGee Eye Institute
2006-2007
University of Oklahoma Health Sciences Center
2007
Preparation of large quantity and high quality genomic DNA from a number plant samples is major bottleneck for most genetic analyses, such as, mapping, TILLING (Targeting Induced Local Lesion IN Genome), next-generation sequencing directly sheared DNA. A variety preparation methods commercial kits are available. However, they either low throughput, yield, or costly. Here, we describe method throughput isolation sorghum [Sorghum bicolor (L.) Moench] leaves dry seeds with quality, affordable...
Summary The eskimo1 ( esk1 ) mutation of Arabidopsis resulted in a 5.5°C improvement freezing tolerance the absence cold acclimation. Here we show that increase is not associated with any ability to survive drought or salt stresses, which are similar their induction cellular dehydration. Genome‐wide comparisons gene expression between esk1‐1 and wild type indicate mutations at result altered transcription factors signaling components set stress‐responsive genes. Interestingly, list 312 genes...
Through sos3 (salt overly sensitive 3) suppressor screening, two allelic mutants that are weak alleles of the strong sos3hkt1-1 were recovered. Molecular characterization identified T-DNA insertions in distal promoter region Arabidopsis thaliana HKT1 (AtHKT1, At4g10310) these suppressors, which results physical separation a tandem repeat from proximal AtHKT1 promoter. The is approximately 3.9 kb upstream ATG start codon and functions as an enhancer element to promote reporter gene...
Sorghum (Sorghum bicolor) is a versatile C4 crop and model for research in family Poaceae. High-quality genome sequence available the elite inbred line BTx623, but functional validation of genes remains challenging due to limited genomic germplasm resources comprehensive analysis induced mutations. In this study, we generated 6400 pedigreed M4 mutant pools from EMS-mutagenized BTx623 seeds through single-seed descent. Whole-genome sequencing 256 phenotyped lines revealed >1.8 million...
Plants are constantly challenged with various abiotic stresses in their natural environment. Elevated temperatures have a detrimental impact on overall plant growth and productivity. Many plants increase tolerance to high through an adaptation response known as acquired thermotolerance. To identify the mechanisms that evolved cope temperature stress, we isolated series of Arabidopsis mutants defective acquisition thermotolerance after exposure 38 degrees C, treatment induces wild-type...
Summary Plants, as sessile organisms, employ multiple mechanisms to adapt the seasonal and daily temperature fluctuations associated with their habitats. Here, we provide genetic physiological evidence that FtsH11 protease of Arabidopsis contributes overall tolerance plant elevated temperatures. To identify various thermotolerance in plants, isolated a series thaliana thermo‐sensitive mutants ( atts ) fail acquire after pre‐conditioning at 38°C. Two allelic mutants, atts244 atts405 , were...
SorghumBase provides a community portal that integrates genetic, genomic, and breeding resources for sorghum germplasm improvement. Public research development in agriculture rely on proper data resource sharing within stakeholder communities. For plant breeders, agronomists, molecular biologists, geneticists, bioinformaticians, centralizing desirable into user-friendly hub crop systems is essential successful collaborations breakthroughs development. Here, we present the web (...
Heat stress reduces grain yield and quality worldwide. Enhancing heat tolerance of crops at all developmental stages is one the essential strategies required for sustaining agricultural production especially as frequency temperature extremes escalates in response to climate change. Although mechanisms have been studied extensively model plant species, little known about genetic control underlying responses crop plants vegetative stage under field conditions. To dissect basis sorghum [ (L.)...
Photosynthetic systems are known to be sensitive high temperature stress. To maintain a relatively "normal" level of photosynthetic activities, plants employ variety adaptive mechanisms in response environmental fluctuations. Previously, we reported that the chloroplast-targeted AtFtsH11 protease played an essential role for Arabidopsis survive at temperatures and normal efficiency moderately elevated temperature. investigate factors contributing changes FtsH11 mutant, performed detailed...
As in other cereal crops, the panicles of sorghum (Sorghum bicolor (L.) Moench) comprise two types floral spikelets (grass flowers). Only sessile (SSs) are capable producing viable grains, whereas pedicellate (PSs) cease development after initiation and eventually abort. Consequently, grain number per panicle (GNP) is lower than total flowers produced panicle. The mechanism underlying this differential fertility not well understood. To investigate issue, we isolated a series ethyl methane...
Sorghum (Sorghum bicolor) is the fifth most important cereal crop worldwide; however, its utilization in food products can be limited due to reduced nutritional quality related amino acid composition and protein digestibility cooked products. Low essential levels are influenced by of sorghum seed storage proteins, kafirins. In this study, we report a core collection 206 mutant lines with altered proteins. Wet lab chemistry analysis was conducted evaluate total content 23 acids, including 19...
The mycotoxin fumonisin B 1 , originally described as being produced by Fusarium moniliforme was detected in liquid cultures of Alternaria alternata f. sp. lycopersici a host-specific pathogen tomato plants. metabolite high-pressure chromatography and mass spectrometry. Its identity confirmed fast atom bombardment ion spray spectrometry, well parent-daughter tandem In three separate experiments, the concentrations found ranged between 5 140 ppm (μg/ml).
Maize ( Zea mays L.) germplasm exhibits large genetic variations in tolerance to high temperature (HT) stress under field conditions, but the mechanisms underling this variation are largely unknown. Based on many years of observation, maize inbred line B76 consistently displays better HT than B106. Heat waves during growing season cause leaf firing developing leaves and tassel blasting B106 not B76. The difference between two lines was confirmed growth chambers controlled conditions. showed...
ABSTRACT The presence of preflowering or postflowering drought tolerance has been described in sorghum [ Sorghum bicolor (L.) Moench]. lines with tend to senesce under stress while (the stay‐green trait) be sensitive stress. Assessments these phenotypes have dependent on the incidence conditions at specific developmental stages achieve meaningful evaluation. Therefore, field‐based evaluations, most which are visual, notoriously difficult perform and require growing multiple locations across...
Sorghum (Sorghum bicolor Moench, L.) plant accumulates copious layers of epi-cuticular wax (EW) on its aerial surfaces, to a greater extent than most other crops. EW provides vapor barrier that reduces water loss, and is therefore considered be major determinant sorghum's drought tolerance. However, little known about the genes responsible for accumulation in sorghum. We isolated two allelic mutants, bloomless40-1 (bm40-1) bm40-2, from mutant library constructed ethyl methane sulfonate (EMS)...
FTSH proteases are membrane-bound, ATP-dependent metalloproteases found in bacteria, mitochondria and chloroplasts. The product of one the 12 genes encoding Arabidopsis, FTSH11, has been previously shown to be essential for acquired thermotolerance. However, substrates this protease, as well mechanism linking it thermotolerance largely unknown. To get insight into these, FTSH11 knockout mutant was complemented with proteolytically active or inactive variants tagged HA-tag, under control...
This report extends research on Al-induced changes in membrane behavior of intact root cortex cells Northern red oak (Quercus rubra). Membrane permeability was determined by the plasmometric method for individual at temperatures from 2 or 4 to 35°C. Al (0.37 millimolar) significantly increased urea and monoethyl decreased water. altered activation energy required transport water (+32%), (+9%), (−7%) across cell membranes. Above 9°C, lipid partiality membranes; below 7°C, it. narrowed 6°C...
Polyamines involve in gene regulation by interacting with and modulating the functions of various anionic macromolecules such as DNA, RNA proteins. In this study, we identified an important function polyamine transporter LHR1 (LOWER EXPRESSION OF HEAT RESPONSIVE GENE1) heat-inducible expression Arabidopsis thaliana. The lhr1 mutant was isolated through a forward genetic screening for altered luciferase reporter driven promoter from AtHSP18.2. showed reduced induction response to heat stress...
Grain number per panicle is an important component of grain yield in sorghum (Sorghum bicolor (L.)) and other cereal crops. Previously, we reported that mutations multi-seeded 1 (MSD1) MSD2 genes result a two-fold increase due to the restoration fertility pedicellate spikelets, which invariably abort natural accessions. Here, report identification another gene, MSD3, also involved regulation numbers sorghum. Four bulked F2 populations from crosses between BTx623 each independent msd mutants...
Mutant populations are crucial for functional genomics and discovering novel traits crop breeding. Sorghum, a drought heat-tolerant C4 species, requires vast, large-scale, annotated, sequenced mutant resource to enhance improvement through research. Here, we report sorghum large-scale population with 9.5 million ethyl methane sulfonate (EMS)-induced mutations that covered 98% of sorghum's annotated genes using inbred line BTx623. Remarkably, total 610 320 within the promoter enhancer regions...
Sorghum (Sorghum bicolor L. Moench) is one of the most important grain crops in world. The nuclear male sterility (NMS) trait, which caused by mutations on gene, valuable for hybrid breeding and genetic studies. Several NMS mutants have been reported previously, but none them were well characterized. Here, we present our detailed morphological characterization a new easily recognizable sorghum mutant sterile 8 (ms8) isolated from an elite inbred BTx623 mutagenized ethyl methane sulfonate...
Grain size is a major determinant of grain yield in sorghum and other cereals. Over 100 quantitative trait loci (QTLs) have been identified sorghum. However, no gene underlying any QTL has cloned. Here, we describe the fine mapping cloning one QTL. From an F8 recombinant inbred line population derived from cross between lines 654 LTR108, 44 QTLs. One QTL, qTGW1a, was detected consistently on long arm chromosome 1 span 4 years. Using extreme recombinants F2:3 fine-mapping population, qTGW1a...