This study presents the fabrication of an ultralight, porous, and high-performance triboelectric nanogenerator (TENG) utilizing silk fibroin (SF) aerogels PDMS sponges as friction layer. The transition from two-dimensional film layers to three-dimensional porous significantly increased specific surface area, offering effective strategy for designing SF aerogel-based TENGs. TENG incorporating aerogel exhibited optimal output performance at a 3% concentration, achieving maximum open circuit...

It has been shown that whole grains and dietary fiber are important for their fermentation characteristics in the large intestine, drawing more attention to quinoa polysaccharides. In this study, we evaluated prebiotic effect of seeds polysaccharides after human simulated digestion. The modulatory (QPs) on gut microbiota was by vitro using fecal microbiota. yield extraction 15.45%. digestibility cooked uncooked simulation digestion 69.04% 64.09%, respectively. composition metabolic products...

Gynura divaricata (L.) DC is a long-used medicinal and edible plant in China folk. Its hyperglycemic effects have garnered increasing public attention recent years. This study revealed that the ethyl acetate (EtOAc) butanol (BuOH) partition fractions of G. crude extract exhibited significantly higher α-glucosidase inhibition activity enhanced glucose uptake ability compared to other fractions. Guided by hypoglycemic bioassay, these two were subjected isolation active compounds using...

Abstract The extraction condition of curcumin from Curcuma longa L was optimized through four factors and three levels orthogonal experiment based on the results single factor tests. Under optimal conditions: concentration ethanol 80%, temperature 70°C, ratio liquid to material 20, time 3 h, a crude extract with yield 56.8 mg/g could be obtained. isolation purification curcuminoids performed high performance counter current chromatography employing an solvent system n ‐hexane/ethyl...

Chlorogenic acid is a secondary metabolite produced by many traditional Chinese medicines. Its physiological activities (antibacterial, anti-inflammatory, antioxidant activities, etc.) have been well described. This study aimed to investigate the effects of chlorogenic on biofilm drinking water bacteria. The metabolites biofilms were also evaluated. was found an anti-biofilm effect against Pseudomonas, resulting in formation dose-dependent manner (0.53-25.4 mM CGA). Moreover, structure...

ABSTRACT MICU2 has been reported to interact with MICU1 and participate in the regulation of mitochondrial Ca2+ uptake, although molecular determinants underlying function is unknown. In order characterize we screened a series N-terminal C-terminal truncations obtained constructs which can be expressed abundance, giving rise soluble samples enable subsequent characterizations. Size exclusion chromatography (SEC) multi-angle laser light scattering (MALLS) revealed that exists as monomer...

Abstract Lanosterol is a potential drug for cataracts treatment, which can reverse the aggregation of intracrystalline proteins. The low concentration in lanolin calls high‐performance separation methods. In this study, counter‐current chromatography dual‐mode elution method was developed first time to separate and purify lanosterol from hexane extract after saponification, column eluted with lower phase as mobile head‐to‐tail mode, followed by upper tail‐to‐head mode. High purity...

Countercurrent Chromatography (CCC), a form of liquid–liquid chromatography, is an efficient preparative purification technique used for purifying active natural compounds, specifically those present in plants. The CCC relies on the use suitable solvent system that can be chosen from diverse options. solvents, spanning large polarity range and selected different families, are compound separation. selection procedure laborious time-consuming. Different strategies, which help simplify method...

Abstract Counter‐current chromatography (CCC) was investigated as a new sample pretreatment method for the determination of trace polycyclic aromatic hydrocarbons (PAHs) in water environmental samples. The experiment performed with non‐aqueous binary two‐phase solvent system composed n ‐heptane and acetonitrile. CCC column first filled upper stationary phase, then large volume pumped into while rotated at 1600 rpm. Finally, amounts PAHs extracted enriched phase were eluted out by lower...

Abstract The evidence for unique effects of eicosapentaenoic acid and docosahexaenoic is growing. Further understanding exploration their independent in the nutraceutical pharmaceutical industry calling more efficient separation techniques to overcome equivalent chain length rule fatty acids. In this study, free were successfully separated by pH‐zone‐refining countercurrent chromatography first time. different solvent systems influence retainer eluter concentration on efficiency...

Abstract An improved type‐J counter‐current chromatography (CCC) planet centrifuge with two spiral tube columns (volume 2×15 mL, β value 0.3–0.7, tubing 0.8 mm id) was developed and evaluated for its retention ability of four typical different solvent systems including heptane–methanol (1:1, v/v) (A), hexane–ethyl acetate–methanol–water (1:1:1:1, (B), n ‐butanol–acetic acid–water (4:1:5, (C), PEG1000–K 2 HPO 4 –water (12.5:12.5:75, w/w) (D) under eight operation modes. The results indicated...

Abstract α‐Linolenic acid is an essential omega‐3 fatty needed for human health. However, the isolation of high‐purity α‐linolenic from plant resources challenging. The preparative separation methods by both conventional and pH‐zone refining counter current chromatography were firstly established in this work. successful was achieved optimized solvent system n ‐heptane/methanol/ water/acetic (10:9:1:0.04, v/v), producing 466 mg 98.98% 900 free sample prepared perilla seed oil with linoleic...

DicB, a protein encoded by the Kim (Qin) prophage in Escherichia coli , inhibits cell division through interaction with MinC. Thus far, characterization of DicB has been severely hampered owing to its potent activity which ceases and leads death. In this work, fusing maltose-binding N-terminus (MBP–DicB), we successfully expressed purified recombinant that enabled vitro analysis for first time. More importantly, taking advantage reduced inhibitory MBP–DicB, were able study effects on growth...

□ Luvangetin, a coumarin compound was separated from the extract of stem bark Zanthoxylum ailanthoides Sieb. & Zucc. by centrifugal partition chromatography (CPC) using two-phase solvent system composed n-hexane–ethyl acetate–methanol–water (2:2:2:1, v/v). Its chemical structural elucidated electrospray ionization mass spectrometry,1 H Nuclear Magnetic Resonance (NMR), and 13C NMR. The ethanol Z. fractioned extraction with petroleum ether, ethyl acetate, n-butanol successively. ether...