A5024154990- SARS-CoV-2 and COVID-19 Research
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Biosensors and Analytical Detection
- Immune Response and Inflammation
- Virus-based gene therapy research
- vaccines and immunoinformatics approaches
- RNA and protein synthesis mechanisms
- COVID-19 epidemiological studies
- Viral Infections and Vectors
- Animal Diversity and Health Studies
- Mosquito-borne diseases and control
- Malaria Research and Control
- Advanced biosensing and bioanalysis techniques
- Travel-related health issues
- Long-Term Effects of COVID-19
- Immune Cell Function and Interaction
- Viral Infections and Immunology Research
- Escherichia coli research studies
University of Gothenburg
2025
King Abdulaziz University
2015-2024
Ain Shams University
2024
King Fahad Hospital Jeddah
2022-2024
University of Jeddah
2023
King Fahd Military Medical Complex
2021-2022
King Abdul Aziz University Hospital
2019
King Fahd Medical City
2019
Health Canada
2009-2015
Faculty (United Kingdom)
2015
We describe the isolation and sequencing of Middle East respiratory syndrome coronavirus (MERS-CoV) obtained from a dromedary camel patient who died laboratory-confirmed MERS-CoV infection after close contact with camels that had rhinorrhea. Nasal swabs collected one his nine were positive for RNA. In addition, was isolated camel. The full genome sequences two isolates identical. Serologic data indicated circulating in but not before human occurred. These suggest this fatal case transmitted...
The COVID-19 pandemic caused by SARS-CoV-2 affects all aspects of human life. Detection platforms that are efficient, rapid, accurate, specific, sensitive, and user friendly urgently needed to manage control the spread SARS-CoV-2. RT-qPCR based methods gold standard for detection. However, these require trained personnel, sophisticated infrastructure, a long turnaround time, thereby limiting their usefulness. Reverse transcription-loop-mediated isothermal amplification (RT-LAMP), one-step...
Dengue viruses (DENVs) are mosquito-borne which can cause disease ranging from mild fever to severe dengue infection. These endemic in several tropical and subtropical regions. Multiple outbreaks of DENV serotypes 1, 2 3 (DENV-1, DENV-2 DENV-3) have been reported the western region Saudi Arabia since 1994. Strains at least two genotypes DENV-1 (Asia America/Africa genotypes) circulating until 2006. However, all previous studies were based on partial sequencing data envelope (E) gene without...
Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel betacoronavirus that has been circulating in the Arabian Peninsula since 2012 and causing severe infections humans. While bats were suggested to be involved human MERS-CoV infections, direct link between uncertain. On other hand, serological virological data suggest dromedary camels as potential animal reservoirs of MERS-CoV. Recently, we isolated from camel its infected owner provided evidence for transmission patient. Here,...
Abstract As the Coronavirus Disease 2019 (COVID-19), which is caused by novel SARS-CoV-2, continues to spread rapidly around world, there a need for well validated serological assays that allow detection of viral specific antibody responses in COVID-19 patients or recovered individuals. In this study, we established and used multiple indirect Enzyme Linked Immunosorbent Assay (ELISA)-based study response patients. order validate determined cut off values, sensitivity specificity using sera...
Abstract MERS-coronavirus is a novel zoonotic pathogen which spread rapidly to >25 countries since 2012. Its apparent endemicity and the wide of its reservoir host (dromedary camels) in Arabian Peninsula highlight ongoing public health threat this virus. Therefore, development effective prophylactic vaccine needs be urgently explored given that there are no approved prophylactics or therapeutics for humans animals date. Different candidates have been investigated but serious safety...
Abstract MERS-CoV seronegative and seropositive camels received a single intramuscular dose of ChAdOx1 MERS, replication-deficient adenoviral vectored vaccine expressing spike protein, with further groups receiving control vaccinations. Infectious active naturally acquired infection, were co-housed the vaccinated at ratio 1:2 (infected:vaccinated); nasal discharge virus titres monitored for 14 days. Overall, vaccination reduced shedding (p = 0.0059 p 0.0274, respectively). Antibody responses...
Abstract Monitoring SARS-CoV-2 spread and evolution through genome sequencing is essential in handling the COVID-19 pandemic. Here, we sequenced 892 genomes collected from patients Saudi Arabia March to August 2020. We show that two consecutive mutations (R203K/G204R) nucleocapsid (N) protein are associated with higher viral loads patients. Our comparative biochemical analysis reveals mutant N displays enhanced RNA binding differential interaction key host proteins. found increased of GSK3A...
Abstract Background Infection control measures have played a major role in limiting human/camel-to-human transmission of Middle East respiratory syndrome coronavirus (MERS-CoV); however, development effective and safe human or camel vaccines is warranted. Methods We extended optimized our previous recombinant adenovirus 5 (rAd5)–based vaccine platform characterized by vivo amplified CD40-mediated specific responses to generate MERS-CoV S1 subunit-based vaccine. generated rAd5 constructs...
SummaryBackgroundThe Middle East respiratory syndrome coronavirus (MERS-CoV) is a lethal zoonotic pathogen endemic to the Arabian Peninsula. Dromedary camels are likely source of infection and virus probably originated in Africa. We studied genetic diversity, geographical structure, prevalence, age-associated prevalence among at largest entry port from Africa into Peninsula.MethodsIn this prospective genomic study, we took nasal samples imported Sudan Djibouti Port Jeddah Jeddah, Saudi...
The Coronavirus Disease 2019 (COVID-19), caused by SARS-CoV-2, continues to spread globally with significantly high morbidity and mortality rates. Antigen-specific responses are of unquestionable value for clinical management COVID-19 patients. Here, we investigated the kinetics IgM, IgG against spike (S) nucleoproteins (N) proteins their neutralizing capabilities in hospitalized patients different disease presentations (i.e., mild, moderate or severe), need intensive care units (ICU)...
Emerging highly pathogenic human coronaviruses (CoVs) represent a serious ongoing threat to the public health worldwide. The spike (S) proteins of CoVs are surface glycoproteins that facilitate viral entry into host cells via attachment their respective cellular receptors. S protein is believed be major immunogenic component and target for neutralizing antibodies (nAbs) most candidate vaccines. Development safe convenient assay thus urgently needed determine prevalence nAbs in population,...
Background: Massive vaccination campaigns have been undertaken globally to combat the spread of Coronavirus Disease 2019 (COVID-19). While most COVID-19 vaccines shown excellent efficacy and safety profiles in clinical studies, real-world monitoring vaccine is still important. In this study, we aimed investigate early side effects Pfizer-BioNTech (BNT162b2) mRNA children between 12-18 years old Saudi Arabia. Method: To age range following administration either one or two doses vaccine,...
BACKGROUND Middle East respiratory syndrome‐coronavirus (MERS‐CoV) is a novel zoonotic pathogen. Although the potential for MERS‐CoV transmission through blood transfusion not clear, was recognized as pathogen of concern safety supply especially after its detection in whole blood, serum, and plasma infected individuals. Here we investigated efficacy amotosalen ultraviolet A light (UVA) to inactivate fresh‐frozen (FFP). STUDY DESIGN AND METHODS Pooled FFP units were spiked with recent...
Estimated seroprevalence of Coronavirus Infectious Disease 2019 (COVID-19), caused by the Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) is a critical evidence for better evaluation virus spread and monitoring progress COVID-19 pandemic in population. In Kingdom Saudi Arabia (KSA), SARS-CoV-2 has been reported specific regions, but an extensive nationwide study not reported. Here, we report to determine prevalence population KSA during pandemic, using serum samples from healthy...